Production of aChaetomium globosumEnolase Monoclonal Antibody

Abstract

Chaetomium globosum is a hydrophilic fungal species and a contaminant of water-damaged building materials in North America. Methods to detect Chaetomium species include subjective identification of ascospores, viable culture, or molecular-based detection methods. In this study, we describe the production and initial characterization of a monoclonal antibody (MAb) for C. globosum enolase. MAb 1C7, a murine IgG 1 isotype MAb, was produced and reacted with recombinant C. globosum enolase (rCgEno) in an enzyme-linked immunosorbent assay and with a putative C. globosum enolase in a Western blot. Epitope mapping showed MAb 1C7 specific reactivity to an enolase decapeptide, LTYEELANLY, that is highly conserved within the fungal class Sordariomycetes. Cross-reactivity studies showed MAb 1C7 reactivity to C. atrobrunneum but not C. indicum. MAb 1C7 did not react with enolase from Aspergillus fumigatus, which is divergent in only two amino acids within this epitope. The results of this study suggest potential utility of MAb 1C7 in Western blot applications for the detection of Chaetomium and other Sordariomycetes species.

The Sordariomycetes: an expanding resource with Big Data for mining in evolutionary genomics and transcriptomics

“…are cosmopolitan saprotrophs and endophytes that are capable of dominating diverse ecological niches, including some extreme environments, evoking interest in their genomic adaptation for survival and dispersal. Species of Chaetomium are common contaminants in indoor environments and are considered to be health hazards ( Andersen et al., 2011 ; Miller and McMullin, 2014 ), causing symptoms of rhinitis and asthma when they infect humans ( Mackenzie, 1979 ; Vesper et al., 2007 ; Hassett et al., 2009 ; Green et al., 2014 ). C. globosum is a model for the industrial production of secondary metabolites, and is also generally considered to be a species complex ( Asgari and Zare, 2011 ; Wang et al., 2016b ).…”

Aureonitols A and B, Two New C13‐Polyketides from Chaetomium globosum, an Endophytic Fungus in Salvia miltiorrhiza

“…Chaetomium globosum , belonging to a well‐known member of the Chaetomiaceae family, was frequently found in plants, soil, animals, and dung . Previously phytochemical studies have afforded a wide range of bioactive secondary metabolites, exemplified as polysaccharides with anticancer and antibacterial activities from C. globosum CGMCC 6882, cytochathiazines A–C possessing a 2 H ‐1,4‐thiazine functionality from the coculture of C. globosum and Aspergillus flavipes , antibacterial cytochalasan alkaloids from the arthropod‐associated fungus C. globosum TW1‐1, aureochaeglobosins A–C as the novel [4+2] cycloaddition heterodimers of chaetoglobosin with significant cytotoxicity against human MDA‐MB‐231 cancer cells from C. globosum from the tuber of Pinellia ternata , antiproliferative cytoglobosins H and I from deep‐sea‐derived fungus C. globosum .…”