Abstract:Background: There is growing interest both in testing IgE in nasal secretions (NS) and in molecular diagnosis of seasonal allergic rhinitis (SAR). Yet, the reliability of nasal IgE detection with the newest molecular assays has never been assessed in a large cohort of pollen allergic patients.Objective: To investigate with microarray technology and compare the repertoires of specific IgE (sIgE) antibodies in NS and sera of a large population of children and adults with SAR.Methods: Nasal secretions were collec… Show more
“…The objective of the study published by Sveva Castelli et al was to investigate the repertoires of sIgE antibodies in nasal secretions and sera of children and adults with allergic rhinitis by using microarray technology. 15 IgE antibodies, mostly to airborne allergens, were found in the nasal secretions in 68.3% of the patients, and the authors concluded that they reflect, at population level, the systemic sensitization profiles. However, their data also suggested that sIgE detection in nasal secretions with the biochip used in the study was not sensitive enough to substitute for the serum sIgE measurements.…”
Section: Vincent Ojwangmentioning
confidence: 98%
“… The third study presented in this editorial relates to the diagnosis of allergies and more particularly how the measurement of organ‐specific IgE may relate to the disease. The objective of the study published by Sveva Castelli et al was to investigate the repertoires of sIgE antibodies in nasal secretions and sera of children and adults with allergic rhinitis by using microarray technology . IgE antibodies, mostly to airborne allergens, were found in the nasal secretions in 68.3% of the patients, and the authors concluded that they reflect, at population level, the systemic sensitization profiles.…”
Early optimal management of asthma is a significant factor in the long-term outcome of children with poorly controlled asthma. The review article opening this issue of Pediatric Allergy and Immunology written by Hamelmann et al addresses this burning issue and discusses the optimal use of biomarkers and lung function testing for the long-term follow-up of these children. 1 In particular, the authors emphasize that determinants of reduced lung function and COPD are found in early life, and should foster better control of childhood asthma. Indeed, adequate asthma control reduces the risk of severe asthma attacks in children and offers the best practice for prevention of later loss of lung function.
“…The objective of the study published by Sveva Castelli et al was to investigate the repertoires of sIgE antibodies in nasal secretions and sera of children and adults with allergic rhinitis by using microarray technology. 15 IgE antibodies, mostly to airborne allergens, were found in the nasal secretions in 68.3% of the patients, and the authors concluded that they reflect, at population level, the systemic sensitization profiles. However, their data also suggested that sIgE detection in nasal secretions with the biochip used in the study was not sensitive enough to substitute for the serum sIgE measurements.…”
Section: Vincent Ojwangmentioning
confidence: 98%
“… The third study presented in this editorial relates to the diagnosis of allergies and more particularly how the measurement of organ‐specific IgE may relate to the disease. The objective of the study published by Sveva Castelli et al was to investigate the repertoires of sIgE antibodies in nasal secretions and sera of children and adults with allergic rhinitis by using microarray technology . IgE antibodies, mostly to airborne allergens, were found in the nasal secretions in 68.3% of the patients, and the authors concluded that they reflect, at population level, the systemic sensitization profiles.…”
Early optimal management of asthma is a significant factor in the long-term outcome of children with poorly controlled asthma. The review article opening this issue of Pediatric Allergy and Immunology written by Hamelmann et al addresses this burning issue and discusses the optimal use of biomarkers and lung function testing for the long-term follow-up of these children. 1 In particular, the authors emphasize that determinants of reduced lung function and COPD are found in early life, and should foster better control of childhood asthma. Indeed, adequate asthma control reduces the risk of severe asthma attacks in children and offers the best practice for prevention of later loss of lung function.
“…Microarrayed allergens can be used for measuring simultaneously IgE reactivity to a large number of different allergen molecules with very small volumes of serum. In this context, it should be mentioned that it has been shown that allergen-specific IgE can be also measured in plasma and other body fluids such as nasal secretions and in milk samples ( 32 – 35 ). Moreover, it has been shown that dried blood spots on paper can be recovered for specific IgE and IgG measurements which allows sending serum samples as paper dried blood spots in simple envelops without requiring expensive packaging, cooling, and sophisticated transportation ( 36 ).…”
Section: Measurement Of Allergen-specific Ige and Igg Responses With mentioning
More than 30% of the world population suffers from allergy. Allergic individuals are characterized by the production of immunoglobulin E (IgE) antibodies against innocuous environmental allergens. Upon allergen recognition IgE mediates allergen-specific immediate and late-phase allergic inflammation in different organs. The identification of the disease-causing allergens by demonstrating the presence of allergen-specific IgE is the key to precision medicine in allergy because it allows tailoring different forms of prevention and treatment according to the sensitization profiles of individual allergic patients. More than 30 years ago molecular cloning started to accelerate the identification of the disease-causing allergen molecules and enabled their production as recombinant molecules. Based on recombinant allergen molecules, molecular allergy diagnosis was introduced into clinical practice and allowed dissecting the molecular sensitization profiles of allergic patients. In 2002 it was demonstrated that microarray technology allows assembling large numbers of allergen molecules on chips for the rapid serological testing of IgE sensitizations with small volumes of serum. Since then microarrayed allergens have revolutionized research and diagnosis in allergy, but several unmet needs remain. Here we show that detection of IgE- and IgG-reactivity to a panel of respiratory allergens microarrayed onto silicon elements is more sensitive than glass-based chips. We discuss the advantages of silicon-based allergen microarrays and how this technology will allow addressing hitherto unmet needs in microarray-based allergy diagnosis. Importantly, it described how the assembly of silicon microarray elements may create different microarray formats for suiting different diagnostic applications such as quick testing of single patients, medium scale testing and fully automated large scale testing.
“…In AR and LAR, sIgE is the principal biomarker that defines the presence of type 2 inflammation. 8 , 9 In AR, sIgE can be detected circulating in the serum or in the mast cells of the skin (systemic atopy), but it can also be found localized in different tissues. 8 , 9 On the other hand, in LAR, sIgE can only be measured in the nasal mucosa (NsIgE).…”
Section: Introductionmentioning
confidence: 99%
“… 8 , 9 In AR, sIgE can be detected circulating in the serum or in the mast cells of the skin (systemic atopy), but it can also be found localized in different tissues. 8 , 9 On the other hand, in LAR, sIgE can only be measured in the nasal mucosa (NsIgE). Other markers (eg, eosinophils, Th2 cells, and some cytokines) have been proposed, 10 but so far none of them is superior to sIgE in detecting the allergenic trigger that induces the inflammatory response.…”
Objectives
Nasal specific IgE (NsIgE) is the most common marker to identify type-2 inflammation in local allergic rhinitis (LAR). However, the comparison of NsIgE in different types of rhinitis, its frequency in tropical countries, and its diagnostic performance for predicting the outcome of a nasal challenge test (NCT) has had limited study. The main objective of this study was to explore the diagnostic performance of NsIgE to
Dermatophagoides pteronyssinus
(Der p) among different types of rhinitis and control subjects in a tropical population.
Methods
We evaluated the frequency of NsIgE, systemic atopy (serum sIgE and Skin Prick Test), and nasal eosinophils, and we performed nasal challenge tests (NCTs) with Der p in 3 groups of patients; rhinitis without atopy (RWoA) (n = 25), rhinitis with atopy (RWA) (n = 25), and control subjects (n = 18).
Results
NsIgE had a low sensitivity and specificity to predict a positive NCT in the RWoA group: 48% had NsIgE, but only 28% had a positive NCT. Among the RWA group 84% had NsIgE and 80% had a positive NCT; the association of NsIgE and positive NCT was high (>80%). In the control group 27.8% had NsIgE, but none had a positive NCT.
Conclusions
NsIgE performs poorly in predicting NCT results in patients with non-allergic rhinitis. More methodical investigations are needed in this complex area of rhinitis. In patients with allergic rhinitis, NsIgE was useful in predicting a positive nasal challenge, but not superior to the systemic atopic test.
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