IG-molecule Kilon shows differential expression pattern from LAMP in the developing and adult rat hippocampus

Bräuer, Anja U.; Savaskan, Nicolai Ε.; Plaschke, Martina; Prehn, Siegfried; Ninnemann, Olaf; Nitsch, Robert

doi:10.1002/1098-1063(2000)10:6<632::aid-hipo1001>3.0.co;2-6

Cited by 13 publications

(6 citation statements)

References 37 publications

(47 reference statements)

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“…The patterns of expression we observe mostly correlate with previously observed expression profiles in specific regions of the brain described in the literature 17 , 21 , 22 , 32 , 51 , 52 , 76 , 77 . However, while we observed expression of IgLON3/Lsamp in the developing cortex at E16, we did not detect its expression in the ventricular zone as previously reported for Lsamp 1b 20 .…”

Section: Discussionsupporting

confidence: 89%

“…Despite IgLONs ’ roles in key neurodevelopmental events, there is fragmented information available in the literature about their expression in certain regions of the developing nervous system 17 , 21 , 22 , 32 , 51 – 53 . We utilized an in situ hybridization approach to create a spatiotemporal map of IgLON mRNA expression in the murine nervous system.…”

Section: Introductionmentioning

confidence: 99%

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Spatiotemporal expression of IgLON family members in the developing mouse nervous system

Fearnley

Raja

Cloutier

2021

Sci Rep

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Differential expression of cell adhesion molecules in neuronal populations is one of the many mechanisms promoting the formation of functional neural circuits in the developing nervous system. The IgLON family consists of five cell surface immunoglobulin proteins that have been associated with various developmental disorders, such as autism spectrum disorder, schizophrenia, and major depressive disorder. However, there is still limited and fragmented information about their patterns of expression in certain regions of the developing nervous system and how their expression contributes to their function. Utilizing an in situ hybridization approach, we have analyzed the spatiotemporal expression of all IgLON family members in the developing mouse brain, spinal cord, eye, olfactory epithelium, and vomeronasal organ. At one prenatal (E16) and two postnatal (P0 and P15) ages, we show that each IgLON displays distinct expression patterns in the olfactory system, cerebral cortex, midbrain, cerebellum, spinal cord, and eye, indicating that they likely contribute to the wiring of specific neuronal circuitry. These analyses will inform future functional studies aimed at identifying additional roles for these proteins in nervous system development.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Spatiotemporal expression of IgLON family members in the developing mouse nervous system

Fearnley

Raja

Cloutier

2021

Sci Rep

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“…Whether such heterophilic interactions are generally inhibitory is not yet known nor is it clear whether Kilon and OBCAM are principally expressed in distinct fiber tracts, or partially overlap in their distribution with Ntm and LAMP. Kilon has been reported to be expressed in the fronto-temporal cortex (Brauer et al, 2000), although at highest levels in cortical layers II and III, based on in situ hybridization (Brauer et al, 2000) or in pyramidal neurons of layer V based on immunocytochemistry (Funatsu et al, 1999). While neurotrimin is abundantly expressed in the frontal cortex, it is predominately expressed in layers IV, V, and VI (Struyk et al, 1995), suggesting that these proteins may contribute to distinct projections.…”

Section: Lamp Inhibits the Outgrowth Of Drg Neuronsmentioning

confidence: 99%

Complementary expression and heterophilic interactions between igLON family members neurotrimin and LAMP

et al. 2002

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Neurotrimin (Ntm) and the limbic system-associated membrane protein (LAMP) are members of the IgLON (LAMP, OBCAM, Ntm) family of glycorylphosphatidylinositol anchored neural cell adhesion molecules. We previously reported that LAMP and Ntm promote adhesion and neurite outgrowth via a homophilic mechanism, suggesting that these proteins promote the formation of specific neuronal circuits by homophilic interactions. In this report, we have further characterized the expression and binding specificity of Ntm. Using a newly generated monoclonal antibody to Ntm, we demonstrated that this protein is largely expressed in a complementary pattern to that of LAMP in the nervous system, with co-expression at a few sites. Ntm is expressed at high levels in sensory-motor cortex and, of particular note, is transiently expressed in neurons of cortical barrel fields and corresponding thalamic "barreloids." Binding of a recombinant, soluble form of Ntm to CHO cells expressing either Ntm or LAMP demonstrates that Ntm and LAMP interact both homophilically and heterophilically. In contrast to conventional growth-promoting activity of Ig superfamily members, LAMP strongly inhibits the outgrowth of Ntm-expressing dorsal root ganglion (DRG) neurons in a heterophilic manner. These anatomical and functional data support the concept that homophilic and heterophilic interactions between IgLON family members are likely to play a role in the specification of neuronal projections via growth promoting and inhibiting effects, respectively.

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“…these proteins are distributed differently in the central nervous systemduringthedevelopmentofneuronsinabrain (11,(13)(14)(15)(16)(17)(18). The IgLON family proteins consist of three Ig domains, the third of which is attached to a glycosylphosphatidylinositol (GPI) anchor.…”

mentioning

confidence: 99%

“…The IgLON superfamily includes the limbic-associated membrane protein (LAMP), the opioid-binding cell adhesion molecule (OBCAM), neurotrimin, and Kilon − , and these proteins are distributed differently in the central nervous system during the development of neurons in a brain ,− . The IgLON family proteins consist of three Ig domains, the third of which is attached to a glycosylphosphatidylinositol (GPI) anchor.…”

mentioning

confidence: 99%

Glycosylation Analysis of IgLON Family Proteins in Rat Brain by Liquid Chromatography and Multiple-Stage Mass Spectrometry

et al. 2008

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IgLON family proteins, including limbic-associated membrane protein (LAMP), opioid-binding cell adhesion molecule (OBCAM), neurotrimin, and Kilon, are immunoglobulin (Ig) superfamily cell adhesion molecules. These molecules are composed of three Ig domains and a glycosylphosphatidylinositol (GPI) anchor and contain six or seven potential N-glycosylation sites. Although their glycosylations are supposed to be associated with the development of the central nervous system like other Ig superfamily proteins, they are still unknown because of difficulty in isolating individual proteins with a high degree of homology in performing carbohydrate analysis. In this study, we conducted simultaneous site-specific glycosylation analysis of rat brain IgLON proteins by liquid chromatography and multiple-stage mass spectrometry (LC-MS ( n )). The rat brain GPI-linked proteins were enriched and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The four proteins were extracted from the gel, and subjected to LC-MS ( n ) after proteinase digestions. A set of glycopeptide MS data, including the mass spectrum, the mass spectrum in the selected ion monitoring mode, and the product ion spectra, was selected from all data based on carbohydrate-related ions in the MS/MS spectrum. The peptide portion and the carbohydrate structure were identified on the basis of peptide-related ion and carbohydrate-related ions, and the accurate mass. The site-specific glycosylations of four proteins were elucidated as follows. N-Glycans near the N-terminal were disialic acid-conjugated complex- and hybrid-type oligosaccharides. The first Ig domains were occupied by Man-5-9. Diverse oligosaccharides, including Lewis a/x-modified glycans, a brain-specific glycan known as BA-2, and Man-5, were found to be attached to the third Ig domain. Three common structures of glycans were found in the GPI moiety of LAMP, OBCAM, and neurotrimin.

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IG-molecule Kilon shows differential expression pattern from LAMP in the developing and adult rat hippocampus

Cited by 13 publications

References 37 publications

Spatiotemporal expression of IgLON family members in the developing mouse nervous system

Spatiotemporal expression of IgLON family members in the developing mouse nervous system

Complementary expression and heterophilic interactions between igLON family members neurotrimin and LAMP

Glycosylation Analysis of IgLON Family Proteins in Rat Brain by Liquid Chromatography and Multiple-Stage Mass Spectrometry

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