Identifying Molecular Markers of Cervical Cancer Based on Competing Endogenous RNA Network Analysis

Abstract

Aim: Recurrence being a major challenge for the treatment of cervical cancer, we aimed at identifying novel molecular markers of cervical cancer to improve recurrence prediction. Methods: Cervical cancer samples were obtained from the Cancer Genome Atlas. Prognosis-associated long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs between recurrent and nonrecurrent samples were acquired using expression analysis. Regulatory relationships among these prognosis-associated RNAs were predicted and used to construct a competing endogenous RNA (ceRNA) network. Key prognostic lncRNAs, miRNAs, and mRNAs were identified using the ceRNA network, followed by the Kaplan-Meier survival analysis to reveal the influence of these key prognostic RNAs on prognosis. Results: In total, 15 lncRNAs, 10 miRNAs, and 348 mRNAs were identified as significant prognosis-associated molecules. The cervical cancer-related ceRNA network contained 13 prognosis-associated lncRNAs, 5 prognosis-associated miRNAs, and 120 prognosis-associated mRNAs. Key prognostic lncRNAs, miRNAs, and mRNAs were further identified using the ceRNA network. The key prognostic lncRNAs included H19 and HOTAIR, those for miRNAs included hsa-miR-133b, hsa-miR-138, and hsa-miR-301b, and those for mRNAs included Wnt family member 2, fibroblast growth factor 7, fibronectin 1, synaptic vesicle glycoprotein 2A, and bone morphogenetic protein 7. Conclusion: The key prognostic lncRNAs, miRNAs, and mRNAs may serve as potential molecular markers for recurrence prediction and may contribute to the treatment of cervical cancer.

The relationship between H19 and parameters of ovarian reserve

“…Circulating H19 has been explored as a diagnostic and prognostic biomarker for other conditions including coronary artery disease [43], multiple myeloma [44], and cancers including bladder and gastric cancer [22,40,41,45,46]. In the reproductive tract, circulating H19 is higher in women with polycystic ovary syndrome (PCOS) compared to controls [47], and H19 has been identified as a potential diagnostic and prognostic marker for epithelial ovarian [48] and cervical cancer [49]. However, a link between circulating H19 and markers of ovarian reserve has not previously been described.…”

Comprehensive analysis of a ceRNA network associated with TP53 mutations in liver hepatocellular carcinoma

“…Multiple gene targets including hsa-miR-133b, AC017104.6, RP5-1092A11.5, RP11-365O16.6 and PTBP1 were prioritized in our ceRNA network construction procedure, some of which were reported to be involved in cancer-speci c ceRNA networks. For example, hsa-miR-133b was identi ed as a prognostic marker in esophageal cancer 48 and cervical cancer 49 . Our results italicized its role in TP53-mutant HCC homeostasis maintenance.…”

Integrated Analysis of ceRNA Regulatory Network Associated With Tumor Stage in Cervical Cancer

“…While many researchers have reported the ceRNA network in cervical cancer (Xia et al, 2018;Du and Chen, 2019;Qin et al, 2019), the molecular mechanism of cervical cancer progression is unknown. Therefore, RNA sequencing data at different cervical cancer stages were downloaded from the cancer genome atlas (TCGA) database to comprehensively understand the ceRNA network during disease progression.…”