Identifikasi Simplisia yang Dijual sebagai Strychnos ligustrina Bl. di Pasar Tradisional Surabaya dengan Metode Random Amplified Polymorphic DNA (RAPD)

Abstract

Authentication of Strychnos ligustrina Bl. had been performed at molecular level (DNA) with Random Amplified Polymorphic DNA (RAPD) method, based on the amplification of random DNA fragments by Polymerase Chain Reaction (PCR) with a single arbitrary primer. The aim of this research was obtaining similar banding patterns between DNA of plant Strychnos ligustrina Bl. and DNA of its lignum on local market. Strychnos ligustrina Bl. was determined by UPT Balai Konservasi Tumbuhan Kebun Raya Purwodadi and plants sold as Strychnos ligustrina Bl. were collected as lignum from traditional market at Wonokromo, Rungkut, Genteng, Benowo dan Pabean. DNA from these plants were extracted by modified Cetyltrimethylammonium bromide (CTAB) method and amplified by RAPD method. Amplification had been performed by primer OPO-4 had shown banding patterns on the gel electrophoresis which banding patterns were shown by Strychnos ligustrina Bl. and plants sold as Strychnos ligustrina Bl. on Benowo. Based on this early result, we assume that plants sold as Strychnos ligustrina Bl. on Benowo has closely genetic relationship with Strychnos ligustrina Bl.

Karakterisasi Morfologis dan Molekuler Varietas Kedelai (Glycine max) untuk Identifikasi Bahan Baku Obat Tradisional Indonesia

“…Produk amplifikasi (amplikon) yang diperoleh, dipisahkan dan divisualisasikan dengan metode elektroforesis hingga menunjukkan adanya pola larik DNA yang karakteristik (7). Penanda RAPD telah digunakan untuk mengidentifikasi tanaman sebagai bahan baku obat tradisional misalnya Strychnos ligustrina (10), Sauropus androgynus (11), Eurycoma longifolia (12), Aloe sp. ( 13), dan Strychnos minor (14).…”

“…Kuantitas DNA isolat yang diperoleh menggunakan Kit Nucleospin secara keseluruhan lebih tinggi dibandingkan isolasi DNA yang menggunakan metode konvensional (10,18). Hal ini diduga karena adanya kolom Nucleospin hijau pada kit.…”