Karakterisasi Morfologis dan Molekuler Varietas Kedelai (Glycine max) untuk Identifikasi Bahan Baku Obat Tradisional Indonesia

Abstract

Kedelai (Glycine max) mengandung banyak nutrisi dan senyawa aktif berkhasiat obat yang berperan penting dalam mengurangi resiko berbagai jenis kanker, penyakit kardiovaskular, masalah pascamenopause, diabetes, dan beberapa gangguan neurodegeneratif. Beberapa kejadian alergi yang disebabkan oleh protein di dalam kedelai menjadi salah satu pemicu perlunya kontrol kualitas kedelai sebagai bahan baku obat tradisional atau produk herbal. Upaya kontrol kualitas kedelai dilakukan dengan karakterisasi morfologis dan karakterisasi molekuler dengan metode Random Amplified Polymorphic DNA (RAPD). Autentikasi molekuler dengan RAPD lebih dapat membedakan 35 varietas kedelai dibandingkan dengan karakterisasi morfologis.

Molecular characterization of red ginger varieties (Zingiber officinale Roxb. var. rubrum) by DNA markers

“…The red ginger rhizome sample underwent particle size reduction prior to the DNA separation method. The methodology employed in this investigation follows the approach outlined in the research conducted by Wiradjaja and Yunita (2021). Their study aimed to decrease the particle size, augmenting the surface area of the sample particles that come into contact with the extraction buffer.…”

“…Sensory testing encounters difficulties when dealing with plant varieties that exhibit various kinds and/or possess morphological and anatomical properties that are not distinct when compared to other herbs, such as Sauropus androgynus (Yunita and Sulisetiorini 2013) and Glycine max (Wiradjaja and Yunita 2021). The process of analyzing herbal chemical ingredients is subject to certain limitations.…”

“…The RAPD method has been employed for various purposes, including the investigation of genetic variation (genotyping) in relation to geographical conditions in plant species such as katuk (Sauropus androgynus) (Yunita dan Sulisetiorini 2013), Aloe sp. (Ezzat et al 2016), dan Strychnos minor (Arumugam et al 2019), Curcuma comosa (Boonsrangsom 2020) and soybeans (Glycine max) (Wiradjaja and Yunita 2021) from diverse geographic regions. This method can also identify plants sold as Strychnos ligustrina in traditional markets (Yunita dan Kresnamurti 2005).…”