Identifikasi Morfologi Spesies Sibling Anopheles vagus vagusdan Anopheles vagus limosusAsal Desa Bangsring, Banyuwangi

Wahyuni, Ika Sri; Senjarini, Kartika; Oktarianti, Rike; Wathon, Syubbanul; Hasanah, Lailly Nur Uswatul

doi:10.23969/biosfer.v3i1.1585

Cited by 2 publications

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“…vagus vagus is characterized by unspotted legs, a proboscis with pale bands, and prehumeral wings with pale bands between two dark bands, whereas An. vagus limosus has characteristic unspotted legs, a dark overall proboscis, and prehumeral wings with dark bands; there is no mixed combination of these characteristics between the two species [ 42 ] Morphology and ecology Siti Alfiah & Mujiyono, 2014 b An.vagus from Semarang, Central Java Indonesia The variations were in the size and the number of hair branches and filaments. This variation in the intra and interpopulation An.…”

Section: Resultsmentioning

confidence: 99%

“…Anopheles vagus vagus is characterized by unspotted legs, a proboscis with pale bands, and prehumeral wings with pale bands between two dark bands, while An. vagus limosus has characteristic unspotted legs, a dark overall proboscis, and prehumeral wings with dark bands [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…Anopheles vagus and An. limosus were distributed sympatricly in several parts of Southeast Asia [ 42 , 45 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

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A systematic review: is Anopheles vagus a species complex?

Dalilah,

Syafruddin,

Saleh

et al. 2024

Malar J

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Background Anopheles vagus (subgenus Cellia) has been identified as a vector for malaria, filariasis, and Japanese encephalitis in Asia. Sporozoites of Plasmodium falciparum and Plasmodium vivax have been found in this zoophilic mosquito in Asia and Indonesia. This study systematically reviews publications regarding An. vagus species, variation, bio-ecology, and malaria transmission in various localities in Asia, especially Indonesia, to determine whether the current data support An. vagus as a species complex. Methods The databases Pubmed, Scopus, Europe PMC, and Proquest were searched to identify information regarding the morphology, karyotypes, polytene chromosome, cross-mating, ecology, and molecular identification of An. vagus was then evaluated to determine whether there were possible species complexes. Results Of the 1326 articles identified, 15 studies were considered for synthesis. The Anopheles spp. samples for this study came from Asia. Eleven studies used morphology to identify An. vagus, with singular studies using each of karyotype identification, chromosomal polytene identification, and cross-breeding experiments. Ten studies used molecular techniques to identify Anopheles spp., including An. vagus. Most studies discovered morphological variations of An. vagus either in the same or different areas and ecological settings. In this review, the members of An. vagus sensu lato grouped based on morphology (An. vagus, An. vagus vagus, An. vagus limosus, and An. limosus), karyotyping (form A and B), and molecular (An. vagus genotype A and B, An. vagus AN4 and AN5). Genetic analysis revealed a high conservation of the ITS2 fragment among members except for the An. vagus genotype B, which was, in fact, Anopheles sundaicus. This review also identified that An. vagus limosus and An. vagus vagus were nearly identical to the ITS2 sequence. Conclusion Literature review studies revealed that An. vagus is conspecific despite the distinct morphological characteristic of An. vagus and An. limosus. Further information using another barcoding tool, such as mitochondrial COI and ND6 and experimental cross-mating between the An. vagus and An. limosus may provide additional evidence for the status of An. vagus as a species complex.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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A systematic review: is Anopheles vagus a species complex?

Dalilah,

Syafruddin,

Saleh

et al. 2024

Malar J

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“…Factors influencing the vectorial capacity of Anopheles species include the density calculation index, human blood index, gonotrophic cycle, and mosquito lifespan, or duration of infective days (Astuti et al, 2016). Sibling species have different vectorial capacities, which can have implications for control measures, highlighting the need for identification processes (Wahyuni et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia

Wathon¹,

Astikaningrum²,

Ardyah³

et al. 2023

Biolokus

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Malaria is an infectious disease caused by Plasmodium and transmitted to humans through the Anopheles mosquito vector. The large diversity of Anopheles in Indonesia, which consists of similar complex species, makes it difficult to identify species based on morphological characteristics. Therefore, it is necessary to have another approach to identifying species based on molecular characteristics using DNA molecular markers. This approach becomes important as a basis for vector-based malaria control efforts. This research aims to explore potential DNA barcodes based on in silico studies as a reference for DNA barcoding analysis of Anopheles species in Indonesia. The data sequences of CO1 and ITS2 from Anopheles sp. were collected on the NCBI database. The sequence alignment was performed using CLUSTALX2. The construction of a phylogenetic tree was carried out using MEGA XI. Data analysis was performed by observing the construction of phylogenetic trees, bootstrap scores, and genetic distance. The data sequence in NCBI shows that 10 species of the genus Anopheles as malaria vectors have been studied in Indonesia. Sequence alignment of the 10 species based on CO1 and ITS2 markers showed that ITS2 markers had higher genetic variation than CO1. Therefore, the ITS2 sequence has the potential to be a DNA barcode for the molecular identification of Anopheles.

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Identifikasi Morfologi Spesies Sibling Anopheles vagus vagusdan Anopheles vagus limosusAsal Desa Bangsring, Banyuwangi

Cited by 2 publications

References 0 publications

A systematic review: is Anopheles vagus a species complex?

A systematic review: is Anopheles vagus a species complex?

In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia

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