Identifikasi Jenis Dan Hubungan Kelimpahan Larva Ikan Dengan Kualitas Air Di Danau Ranau, Sumatera Selatan

Abstract

ABSTRAKIdentifikasi larva ikan seringkali sulit dilakukan. Belum tersedianya kunci identifikasi untuk spesies larva ikan di perairan umum daratan sehingga belum adanya pedoman untuk dapat mengidentifikasi spesies larva ikan secara morfologi maupun meristik. Penelitian ini bertujuan untuk mengidentifikasi spesies larva ikan melalui sekuen DNA sehingga menghasilkan data spesies yang lebih pasti dan hubungan kelimpahan larva ikan dengan parameter kualitas perairan di Danau Ranau, Sumatera Selatan. Lokasi pengambilan sampel identifikasi jenis dan kualitas perairan di enam stasiun di Perairan Danau Ranau, Sumatera Selatan. Hasil identifikasi dengan menggunakan sekuen DNA ditemukan sepuluh jenis larva ikan. Kesepuluh jenis larva ikan tersebut antara lain Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. Sekuen DNA kemudian disejajarkan dengan sekuen referen dari perpustakaan data bank gen untuk mengakar pohon dengan menggunakan program BLAST dari NCBI untuk menemukan identitas yang paling dekat keterkaitannya dengan sampel larva-larva ikan yang dianalisis. Hubungan filogeni spesies diantara takson larvalarva ikan di Danau Ranau berdekatan. Kondisi perairan di Danau Ranau tergolong baik untuk biota perairan seperti larva ikan. Kualitas air merupakan parameter yang menentukan kelimpahan larva ikan di Danau Ranau. Larva-larva ikan antar populasi memiliki jarak genetik yang dekat antar satu dengan yang lain. Kelimpahan larva ikan berkorelasi positif dengan turbiditas dan berkorelasi negatif terhadap parameter daya hantar listrik. Kata Kunci: Larva ikan; Kualitas perairan; Sekuen DNA; Danau Ranau ABSTRACTIdentification of fish larva is often difficult. The unavailability of key identification for fish larva species in inland waters so that there are no guidelines for identifying fish larvae morphologically or meristically. This study aims to identify fish larva species from the Ranau Lake waters, South Sumatra through DNA sequences to produce more definitive species data and relationship of abundance of fish larva and water quality of parameters in Ranau Lake waters, South Sumatera. The sampling location of species identification and water quality was six stations in Ranau Lake waters, South Sumatra. The results of the study found the identification of fish larva species using DNA sequence found ten types of fish larva obtained. The ten types of fish larva include Rasbora argyrotaenia, Puntius tetrazona, Oreochromis mossambicus, Oreochromis niloticus, Gambusia affinis, Poecilia reticulata, Mystacoleucus marginatus, Channa striata, Trichopsis vittata, and Trichogaster trichopterus. The DNA sequence was then aligned with the referent sequence from the gene bank data library to root the tree by using the BLAST program from NCBI to find the identity that was closest to the sample of fish larvae analyzed. The relationship of species phylogeny between the fish l...

Study Identification of Some Species of Fish Using the Partial Fragment of Mitochondrial Cytochrome Oxidase Subunit-1Gene (COI) in Danau Panggang, South Borneo

“…The position of organisms on the phylogenetic tree shows the close kinship between adjacent taxons. Organisms of different species of the same genus will be close to each other in trees, and the further distance in the phylogenetic tree is found in different genera (Wulandari et al, 2019).…”

“…The composition of the PCR reaction was carried out with a final volume of 50 µl consisting of 2 µl of DNA samples, 21 µl of sterile nuclease water (Firstbase), primer of 1 µl each and also the addition of 25 µl of PCR premix (My Taq Bioline red mix). The PCR reaction was carried out using a thermocycler (BIOER) machine with the following conditions: the pra-denaturation stage at a temperature of 95 °C for 1 minute, the second stage consisting of 35 cycles which included a denaturation stage at temperature of 95 °C for 15 seconds for each cycle, the primary attachment (annealing) at a temperature of 55 °C for 15 seconds, extension at a temperature of 72 °C for 30 seconds, and the last stage in the form of final extension at a temperature of 72 °C for 3 minutes and the introduction to renaturation at a temperature of 6 °C for 6 minutes (Wulandari et al, 2019).…”

“…After the electrophoresis had been completed, the gel was put into gel documentation (Protein Simple Alpha Imager), and the gel doc UV lamp was turned on. The analysis of the presence of DNA bands was done by comparing ladder III 100 bp (Firstbase) DNA markers, positive control, and negative control (Wulandari et al, 2019).…”

Sensory Characteristics of Sheredded Spiced Fish Formulations With Different Processing Treatments

“…Danau Ranau memiliki potensi yang sangat baik untuk kehidupan biota akuatik, hal ini ditunjukkan oleh kualitas air seperi suhu, tingkat kekeruhan, pH, oksigen terlarut, fosfat, dan karbondioksida yang masih cukup baik (Herlan & Wulandari, 2021). Beberapa komoditas ikan yang terdapat di Danau Ranau ialah sepat mutiara (Trichogaster trichopterus), tempalo (Gambusia affinis), gupi (Poecilia reticulata), kepiak (Puntius tetrazona), kepiat (Mystacoleucus marginatus), nila (Oreochromis niloticus), mujair (Oreochromis mossambicus), gabus (Channa striata), seluang (Rasbora argyrotaenia), dan sepat siam (Trischopsis vittata) (Herlan & Wulandari, 2021;Wulandari et al, 2019). Namun, jenis ikan yang memiliki potensi tinggi dan paling diminati adalah ikan nila.…”

Fish Identification based on Partial Fragments of The Mitochondrial COI Subunit I Gene

“…In addition, the PCR products were run on a 120v, 250A electrophoresis instrument for 5 minutes, and GelDoc was used for visualization. Use the 100 bp marker [16] to observe PCR results of DNA size. The amplified DNA fragments were separated from an agarose gel (1%) and stained with ethidium bromide.…”