Identification, tissue distribution and characterization of two heat shock factors (HSFs) in goldfish (Carassius auratus)

Kim, So‐Sun; Chang, Ziwei; Park, Jang-Su

doi:10.1016/j.fsi.2015.01.004

Cited by 21 publications

(15 citation statements)

References 47 publications

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“…The heat shock proteins (HSPs) are some of the proteins that show increased abundance during heat stress and other stress conditions. In addition to acting as molecular chaperones, the HSPs play important role in immunomodulation, apoptosis, and various aspects of the inflammatory process . In an earlier investigation, to study the response of different HSPs to high temperature, we studied the expression profiles of a battery of hsp genes in the liver of Channa striatus exposed to high temperature (36°C for various time periods) and compared with that of fish collected from aquaculture ponds (25°C; control) and a hot spring runoff (36–38°C) (described later in discussion) .…”

Section: Introductionmentioning

confidence: 99%

Proteomic changes in the liver of Channa striatus in response to high temperature stress

et al. 2016

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The present study was undertaken to investigate the proteomic changes in the liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36°C for 4 days and liver proteome changes were analyzed using gel- based proteomics, i.e. 2DE, MALDI-TOF/TOF-MS, and validation by transcript analysis. The study showed, besides others, increased abundance of two sets of proteins, the antioxidative enzymes superoxide dismutase (SOD), ferritin, cellular retinol binding protein (CRBP), glutathione-S-transferase (GST), and the chaperones HSP60 and protein disulfide isomerase; this was validated by transcript analysis. The proteome data are available via ProteomeXchange with identifier PXD002608. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer durations (30 days experimental exposure in laboratory and for 30 days beyond, taking Channa collected from a hot spring runoff at 36-38°C); sod, gst, crbp, and hsp60 were found to continue to remain upregulated at eight-, 2.5-, 2.4-, and 2.45-fold, respectively, in the hot spring runoff fish. Pathway analysis showed that the upregulations of the antioxidant enzymes as well as molecular chaperones are induced by the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2). Thus, while short-term heat stress tolerance involves the antioxidative enzymes SOD, ferritin, CRBP, GST, and chaperones HSP60 and protein disulfide isomerase, adaptation under chronic heat stress is associated with SOD, CRBP, GST, and HSP60.

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Section: Introductionmentioning

confidence: 99%

Proteomic changes in the liver of Channa striatus in response to high temperature stress

et al. 2016

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“…This study confirmed that fish and mouse have different activated temperature by thermal stress. Circular dichroism (CD) can screen the changes in the conformation of biopolymers and has previously been used to analyze the effects of heat stress on hmHSF1 and gfHSF1 [12]. In humans, HSF1 undergoes heat-inducible monomer-to-trimer conversion and binds to its cognate regulatory site, the heat shock element (HSE) [27].…”

Section: Resultsmentioning

confidence: 99%

“…The Bio-Rad Mini Trans-Blot Electrophoretic Transfer Cell electroblotted SDS-PAGE samples, including the 42°C and 25°C treated cell pellet, 33°C and 37°C treated cell pellet and control cell pellets in transfer buffer (15.6 mM Tris-HCl, 120 mM glycine, pH 8.3) to the nitrocellulose membrane, were examined. After being blocked with 3% bovine serum albumin (BSA) in Tris-Buffered saline (TBS), the polyclonal antibodies of antigoldfish HSP70, HSF1 and β-actin, which were prepared in this laboratory [4,12], a rat monoclonal antibody HSF1 Ab-1, anti-HSP70 and β-actin were purchased from Thermo scientific and used for specific binding. After treating primary antibodies, secondary antibodies (anti-mouse IgG antibody conjugated with alkaline phosphatase) were diluted at 1:5000.…”

Section: Exposure Of Hepatocytes and Western Blot Analysismentioning

confidence: 99%

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Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Kim¹,

So²,

Park³

2016

Journal of Life Science

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Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at 33°C and showed slightly at 37°C, whereas mouse HSF1 (mHSF1) did so at 42°C. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from 25°C to 42°C. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.

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“…Gene expression after a change in temperature develops in the fish Danio rerio [37,38] and Carassius auratus [44] during the first four hours, and adaptation changes in lipidprotein complexes of synaptic membranes take tens of hours (Fig. 2 and 3).…”

Section: Kinetics Of Temperature Adaptationmentioning

confidence: 99%

Limits of Temperature Adaptation and Thermopreferendum

Aslanidi

Kharakoz

2021

Preprint

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Background Managing the limits of temperature adaptation is relevant both in medicine and in biotechnology. There are numerous scattered publications on the identification of the temperature limits of existence for various organisms and using different methods. It was shown earlier that each cycle of synaptic exocytosis includes reversible phase transitions of lipids of the presynaptic membrane due to the entry and subsequent removal of calcium ions from the synaptic terminal. Violation of cyclic changes in membrane fluidity caused by a sharp increase or decrease in ambient temperatures will lead to the termination of synaptic transmission, which can be recorded by the loss of righting reflex.Results The viability polygon for Danio rerio was determined by the minimum temperature of cold shock (about 6 o C), maximum temperature of heat shock (about 43 o C), and thermopreferendum temperature (about 27 o C). The ratio of the temperature range of cold shock to the temperature range of heat shock was about 1.3. Conclusions The experimental values of the temperatures of cold shock and heat shock and the temperature of the thermal preferendum correspond to the temperatures of phase transitions of the lipid-protein composition of the synaptic membrane between the liquid and solid states. The viability range for zebrafish coincides with the temperature range, over which enzymes function effectively and also coincides with the viability polygons for the vast majority of organisms. The boundaries of the viability polygon are characteristic biological constants. The viability polygon of a particular organism is determined not only by the genome, but also by the physicochemical properties of lipids that make up the membrane structures of synaptic endings. The limits of temperature adaptation of any biological species are determined by the temperature range of the functioning of its nervous system.

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Identification, tissue distribution and characterization of two heat shock factors (HSFs) in goldfish (Carassius auratus)

Cited by 21 publications

References 47 publications

Proteomic changes in the liver of Channa striatus in response to high temperature stress

Proteomic changes in the liver of Channa striatus in response to high temperature stress

Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

Limits of Temperature Adaptation and Thermopreferendum

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