Proteomic changes in the liver ofChanna striatusin response to high temperature stress

Abstract

The present study was undertaken to investigate the proteomic changes in the liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36°C for 4 days and liver proteome changes were analyzed using gel- based proteomics, i.e. 2DE, MALDI-TOF/TOF-MS, and validation by transcript analysis. The study showed, besides others, increased abundance of two sets of proteins, the antioxidative enzymes superoxide dismutase (SOD), ferritin, cellular retinol binding protein (CRBP), glutathione-S-transferase (GST), and the chaperones HSP60 and protein disulfide isomerase; this was validated by transcript analysis. The proteome data are available via ProteomeXchange with identifier PXD002608. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer durations (30 days experimental exposure in laboratory and for 30 days beyond, taking Channa collected from a hot spring runoff at 36-38°C); sod, gst, crbp, and hsp60 were found to continue to remain upregulated at eight-, 2.5-, 2.4-, and 2.45-fold, respectively, in the hot spring runoff fish. Pathway analysis showed that the upregulations of the antioxidant enzymes as well as molecular chaperones are induced by the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2). Thus, while short-term heat stress tolerance involves the antioxidative enzymes SOD, ferritin, CRBP, GST, and chaperones HSP60 and protein disulfide isomerase, adaptation under chronic heat stress is associated with SOD, CRBP, GST, and HSP60.

Liver proteome response of pre-harvest Atlantic salmon following exposure to elevated temperature

“…At elevated temperature, activation of the UPR response was indicated by the up-regulation of chaperones and other proteins required for protein folding and stabilization of pre- and un-folded proteins, specifically calreticulin (CALR), disulfide isomerases (PDIA3, PDIA6), 78 kDa glucose-regulated protein (HSPA5/Bip or GRP78) and ER glycosyltransferases (UGT2A1, RPN1). Our data confirm previous detections of ER stress and UPR in liver of thermal-stressed fish [ 15 , 16 , 33 , 84 ] and demonstrate its specific importance in the adaptive response to chronically elevated temperature. Another indicator of the crosstalk between cellular oxidative stress and ER stress was the 3-fold up-regulation of the collagen-specific chaperone Serpin H1 (SERPINH1 or HSP47) which is also induced by stress induced-lipid peroxidation [ 87 , 88 ].…”

“…The molecular dynamics of adaptation to thermal stress are dependent on tissue type examined, type of stress, and thermal exposure regime [ 11 ]. Specifically in liver tissue, metabolic down-regulation and small magnitude response of stress indicators appears to be associated with chronic exposure to elevated temperature [ 13 , 33 ], while an up-regulated response and larger fold changes are elicited in response to more acute thermal stresses [ 15 , 16 ].…”

“…Specifically, the observed co-regulation of PRDX3 and PRDX4 has been proposed as an outcome of antioxidant response and oxidative damage in cancer [ 71 ]. The 3-fold up-regulation of FTL was similar to the increased transcript and protein abundance in Channa liver upon chronically elevated temperature [ 16 ]. Iron interacts with hydrogen peroxide via the Fenton reaction leading to the production of hydroxyl radical ROS, a very reactive initiator of lipid peroxidation [ 72 ].…”

“…In relation to previous fish (i.e. non-salmon species) proteomics research, the liver response to thermal stress has been only examined using gel-based approach [ 15 , 16 ]. Shotgun proteomics can extend the range of quantifiable proteins and provide a more detailed characterization of the affected mechanisms that are common to different species and thermal stresses.…”

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

“…Up-regulation of RBP enhances uptake of vitamin A to enhance fish immunity and growth [62]. For example, RBP was up-regulated in Channa striatus under high-temperature stress [63], suggesting that RBP has a positive effect on teleosts facing environmental temperature changes. In the vitamin digestion and absorption pathway, pantotherate (vitamin B5) and nicotinamide (vitamin B3) are up-regulated at low temperature, suggesting they might enhance low-temperature tolerance of T. fasciatus along with vitamin C [58].…”