Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single-nucleocapsid baculovirus

Chen, Xinwen; IJkel, W.F.J.; Dominy, Cliff; Zanotto, Paolo Marinho de Andrade; Hashimoto, Yoshifumi; Faktor, Ouriel; Hayakawa, Tohru; Wang, Chung Hsiung; Prekumar, Arumagam; Mathavan, Sinnakaruppan; Krell, Peter J.; Hú, Zhìhóng; Vlak, Just M.

doi:10.1016/s0168-1702(99)00097-0

Cited by 33 publications

(23 citation statements)

References 33 publications

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“…Baculovirus phylogenies have usually been based on individual gene sequences. The polyhedrin/granulin (polh) gene, encoding the major matrix protein of the OBs, has been the most widely used (5,7,14,34,60), but other genes, such as DNA polymerase, egt, gp41, chitinase, cathepsin, and lef2, have also been utilized (7,8,10,12,29,30,34,42). In general, these studies agree that the lepidopteran NPVs and GVs constitute distinct, well-defined groups (7,14,23,24,60).…”

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confidence: 76%

Ancient Coevolution of Baculoviruses and Their Insect Hosts

Herniou

Olszewski

O’Reilly

et al. 2004

J Virol

146

117

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If the relationships between baculoviruses and their insect hosts are subject to coevolution, this should lead to long-term evolutionary effects such as the specialization of these pathogens for their hosts. To test this hypothesis, a phylogeny of the Baculoviridae, including 39 viruses from hosts of the orders Lepidoptera, Diptera, and Hymenoptera, was reconstructed based on sequences from the genes lef-8 and ac22. The tree showed a clear division of the baculoviruses according to the order of their hosts. This division highlighted the need to reconsider the classification of the baculoviruses to include one or possibly two new genera. Furthermore, the specialization of distinct virus lineages to particular insect orders suggests ancient coevolutionary interactions between baculoviruses and their hosts.Coevolution, reciprocal evolution in interacting species driven by natural selection (54), is a major driving factor in the historical associations between pathogens and their hosts (13,25,59). Studies on the evolution of pathogen virulence and host resistance have shown that within populations both pathogens and hosts are able to adapt in response to the interactions (51, 59). However, there is much debate on how these microevolutionary scale changes can influence the patterns of speciation of the interacting species at macroevolutionary levels. Coevolution need not necessarily lead to the cospeciation of the interacting species. However, coevolutionary theories (54) all support the hypothesis that the processes of coadaptations would lead to a general trend of parasite specialization for their hosts (53), regardless of the age of the association. Retroviruses and herpesviruses, with their vertebrate hosts, are good examples of specialist pathogens for which coevolution leading to a certain level of cocladogenesis within subfamilies has been demonstrated (38-40).The family Baculoviridae comprises a diverse group of arthropod-specific DNA viruses. They have been reported worldwide from over 600 host species (37), mostly from insects of the order Lepidoptera but also from the orders Diptera, Hymenoptera, and the crustacean order Decapoda (6, 16). The family Baculoviridae is currently subdivided into two genera based on several criteria, including the morphology of the occlusion bodies (OBs) and on mechanisms of nucleocapsid envelop-

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confidence: 76%

Ancient Coevolution of Baculoviruses and Their Insect Hosts

Herniou

Olszewski

O’Reilly

et al. 2004

J Virol

146

117

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“…The HaSNPV has been extensively used for the control of H. armigera since the first isolation of this pathogen in Hubei province of China. The genome of this virus has been completely sequenced ) and several of its genes such as the egt (Chen et al, 1997), lef-2 (Chen et al, 1999), p6.9 ), Ha122 (Long et al, 2003) and Ha94 (Fang et al, 2003) were characterized. In this paper, we describe the molecular properties of the HaSNPV chitinase gene including temporal transcriptional regulation and immunological properties.…”

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confidence: 99%

Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus

Wang

Deng

et al. 2004

Virus Research

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“…Phylogenetic analysis indicated that HearNPV belongs to the group II NPVs (12,29). Its DNA genome is 131 kb and contains 135 open reading frames (ORFs) that potentially encode proteins of 50 amino acids (aa) or larger (13).…”

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confidence: 99%

Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100

Deng

Wang

Mao

et al. 2007

J Virol

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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry were used to analyze the structural proteins of the occlusion-derived virus (ODV) of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV), a group II NPV. Twenty-three structural proteins of HearNPV ODV were identified, 21 of which have been reported previously as structural proteins or ODV-associated proteins in other baculoviruses. These include polyhedrin, P78/83, P49, ODV-E18, ODV-EC27, ODV-E56, P74, LEF-3, HA66 (AC66), DNA polymerase, GP41, VP39, P33, ODV-E25, helicase, P6.9, ODV/BV-C42, VP80, ODV-EC43, ODV-E66, and PIF-1. Two proteins encoded by HearNPV ORF44 (ha44) and ORF100 (ha100) were discovered as ODV-associated proteins for the first time. ha44 encodes a protein of 378 aa with a predicted mass of 42.8 kDa. ha100 encodes a protein of 510 aa with a predicted mass of 58.1 kDa and is a homologue of the gene for poly(ADP-ribose) glycohydrolase (parg). Western blot analysis and immunoelectron microscopy confirmed that HA44 is associated with the nucleocapsid and HA100 is associated with both the nucleocapsid and the envelope of HearNPV ODV. HA44 is conserved in group II NPVs and granuloviruses but does not exist in group I NPVs, while HA100 is conserved only in group II NPVs.

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Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single-nucleocapsid baculovirus

Cited by 33 publications

References 33 publications

Ancient Coevolution of Baculoviruses and Their Insect Hosts

Ancient Coevolution of Baculoviruses and Their Insect Hosts

Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus

Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100

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