Identification, production and bioactivity of casein phosphopeptides – A review

Tenenbaum, Mathie; Deracinois, Barbara; Dugardin, Camille; Matéos, Aurélie; Romelard, Audrey; Auger, Julie; Boulier, Audrey; Ravallec, Rozenn; Flahaut, Christophe; Cudennec, Benoît

doi:10.1016/j.foodres.2022.111360

Cited by 23 publications

(12 citation statements)

References 191 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the most significant disadvantage of organic calcium is its low calcium content. Peptide‐chelated calcium has therefore attracted much attention as an excellent calcium supplement 4,5 …”

Section: Introductionmentioning

confidence: 99%

“…Peptide-chelated calcium has therefore attracted much attention as an excellent calcium supplement. 4,5 Casein phosphopeptide (CPP) is a biologically active peptide derived from cow's milk with a highly acidified active center (-SerP-SerP-SerP-Glu-Glu-) in the amino acid sequence. 6,7 It has important physiological functions such as promoting the absorption and utilization of mineral elements, 8,9 preventing dental caries, 10 antioxidation, 11 and enhancing immunity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Casein phosphopeptide calcium chelation: preparation optimization, in vitro gastrointestinal simulated digestion, and peptide fragment exploration

Wang,

Bai

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDThe calcium is closely related to the formation of bones and teeth, cell metabolism and other physiological activities. In this work, casein phosphopeptide‐calcium chelate (CPP‐Ca) was synthesized and the optimal process parameters for the chelation reaction were obtained. The bioavailability of calcium in CPP‐Ca was investigated by in vitro gastrointestinal simulated digestion. The existence of phytic acid and oxalic acid in digestion system was evaluated to clarify the calcium holding ability of CPP. The liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was utilized to identify oligopeptides from CPP‐Ca.RESULTSThe optimal process parameters for the chelation reaction follows: peptide concentration 7.76 mg/mL, pH 8.54, and the reaction temperature 43.3°C. The digestion in vitro results indicated that the calcium release rate of CPP‐Ca in the stomach for 2 h reached 85%, and about 50% of the ionized calcium was re‐chelated with CPP in the intestine. The phytic acid and oxalic acid could lead to a sharp decrease in soluble calcium, but around 50% of the calcium was still retained in the form of chelates in the presence of CPP. The liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) identified nineteen casein‐derived oligopeptides after digestion, and calcium modifications were found on 8 peptides derived from β‐casein and αs2‐casein.CONCLUSIONSThe work clarified the excellent calcium holding capacity of CPP in the presence of phytic acid and oxalic acid. Furthermore, LC‐MS/MS revealed peptide changes, and identified peptides with calcium chelation. These findings provided significant insights into further utilization and product development of peptide‐calcium chelate in food industry.This article is protected by copyright. All rights reserved.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Casein phosphopeptide calcium chelation: preparation optimization, in vitro gastrointestinal simulated digestion, and peptide fragment exploration

Wang,

Bai

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…These have been associated with anti-hypertensive, angiotensin-converting enzyme (ACE) inhibitory, antioxidant, anti-inflammatory, and immunomodulatory effects, which could deliver health benefits (Beltrán-Barrientos et al, 2016; Raveschot et al, 2018). Bioactive peptides may also improve mineral bioavailability (Tenenbaum et al, 2022), which could support healthy ageing and the prevention of osteoporosis. Since the neurotransmitter γ-aminobutyric acid (GABA) is one of the by-products of LAB metabolism, it has been hypothesised that FF could influence the brain.…”

Section: Living Foods: Safe Salvation For Healthmentioning

confidence: 99%

Microbiota and probiotics: chances and challenges – a symposium report

Ruxton¹,

Kajita²,

Pot³

2023

Gut. Microb.

View full text Add to dashboard Cite

The 10 th International Yakult Symposium was held in Milan, Italy, on 13-14 October 2022. Two keynote lectures covered the crewed journey to space and its implications for the human microbiome, and how current regulatory systems can be adapted and updated to ensure the safety of microorganisms used as probiotics or food processing ingredients. The remaining lectures were split into sections entitled 'Chances' and 'Challenges'. The 'Chances' section related to opportunities for the science of probiotics and fermented foods to contribute to diverse areas of health such as irritable bowel syndrome, major depression, Parkinson's Disease, immune dysfunction, infant colic, intensive care, respiratory infections, and promoting healthy longevity. The 'Challenges' section included selecting appropriate clinical trial participants and methodologies to minimise heterogeneity in responses, how to view probiotics in the context of One Health, and understanding how substances of bacterial origin can cross the blood-brain barrier. The symposium provided evidence from cutting-edge research that gut eubiosis is vital for human health and, like space, the microbiota deserves further exploration of its vast potential.

show abstract

“…Casein, the main protein in milk, can be hydrolyzed by proteases to produce bioactive peptides, of which CPPs have been extensively studied due to their good solubility, digestive stability, and strong ability to promote calcium absorption ( 13 ). For example, Liu et al found that CPPs can promote the transport of calcium in Caco-2 monolayers ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

“…Pan et al found a significant proliferative effect of casein hydrolysate on osteoblasts (HFOB1.19) ( 17 ). It was also found that because these phosphorylated peptides are negatively charged, they can chelate with some minerals, such as iron and calcium, and have a strong binding capacity, greatly increasing the solubility and bioavailability of minerals ( 13 ). However, current studies on CPP-Ca mainly focus on the ability to promote calcium absorption.…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and osteogenic activity mechanism of casein phosphopeptide-calcium chelate

et al. 2022

View full text Add to dashboard Cite

Casein phosphopeptides (CPPs) are good at calcium-binding and intestinal calcium absorption, but there are few studies on the osteogenic activity of CPPs. In this study, the preparation of casein phosphopeptide calcium chelate (CPP-Ca) was optimized on the basis of previous studies, and its peptide-calcium chelating activity was characterized. Subsequently, the effects of CPP-Ca on the proliferation, differentiation, and mineralization of MC3T3-E1 cells were studied, and the differentiation mechanism of CPP-Ca on MC3T3-E1 cells was further elucidated by RNA sequencing (RNA-seq). The results showed that the calcium chelation rate of CPPs was 23.37%, and the calcium content of CPP-Ca reached 2.64 × 105 mg/kg. The test results of Ultraviolet–Visible absorption spectroscopy (UV) and Fourier transform infrared spectroscopy (FTIR) indicated that carboxyl oxygen and amino nitrogen atoms of CPPs might be chelated with calcium during the chelation. Compared with the control group, the proliferation of MC3T3-E1 cells treated with 250 μg/mL of CPP-Ca increased by 21.65%, 26.43%, and 28.43% at 24, 48, and 72 h, respectively, and the alkaline phosphatase (ALP) activity and mineralized calcium nodules of MC3T3-E1 cells were notably increased by 55% and 72%. RNA-seq results showed that 321 differentially expressed genes (DEGs) were found in MC3T3-E1 cells treated with CPP-Ca, including 121 upregulated and 200 downregulated genes. Gene ontology (GO) revealed that the DEGs mainly played important roles in the regulation of cellular components. The enrichment of the Kyoto Encyclopedia of Genes and Genomes Database (KEGG) pathway indicated that the AMPK, PI3K-Akt, MAPK, and Wnt signaling pathways were involved in the differentiation of MC3T3-E1 cells. The results of a quantitative real-time PCR (qRT-PCR) showed that compared with the blank control group, the mRNA expressions of Apolipoprotein D (APOD), Osteoglycin (OGN), and Insulin-like growth factor (IGF1) were significantly increased by 2.6, 2.0 and 3.0 times, respectively, while the mRNA levels of NOTUM, WIF1, and LRP4 notably decreased to 2.3, 2.1, and 4.2 times, respectively, which were consistent both in GO functional and KEGG enrichment pathway analysis. This study provided a theoretical basis for CPP-Ca as a nutritional additive in the treatment and prevention of osteoporosis.

show abstract

Identification, production and bioactivity of casein phosphopeptides – A review

Cited by 23 publications

References 191 publications

Casein phosphopeptide calcium chelation: preparation optimization, in vitro gastrointestinal simulated digestion, and peptide fragment exploration

Casein phosphopeptide calcium chelation: preparation optimization, in vitro gastrointestinal simulated digestion, and peptide fragment exploration

Microbiota and probiotics: chances and challenges – a symposium report

Preparation, characterization, and osteogenic activity mechanism of casein phosphopeptide-calcium chelate

Contact Info

Product

Resources

About