Identification of yeasts within Saccharomyces sensu stricto complex by PCR-fingerprinting

Santos, Scheila Karina Brito dos; Basílio, Anna Carla Moreira; Brasileiro, Bereneuza Tavares Ramos Valente; Simões, Diogo Ardaillon; Silva-Filho, Eurípedes Alves da; Morais, Marcos de

doi:10.1007/s11274-007-9407-6

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…However, the other four closely species (S. paradoxus, S. cariocanus, S. mikatae and S. kudriavzevii) still lack exclusive primers. The single primer amplification reactions (SPAR) technique has been applied to discriminate this Saccharomyces complex, which was based on the species-specific patterns (dos Santos et al 2007;Huang et al 2008). However, SPAR reproducibility is known to be sensitive to reaction conditions.…”

Section: Discussionmentioning

confidence: 99%

The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

Huang

Lee

Tai

2008

Antonie van Leeuwenhoek

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The Saccharomyces sensu stricto complex comprises seven very closely related species. In this study, we compared the use of two different phylogenetic markers, the 26S rDNA and beta-tubulin genes, for discriminating phylogenetic relationships among Saccharomyces sensu stricto strains using sequencing as well as RFLP methods. The average sequence similarity for the beta-tubulin gene (90.0%) among seven strains was significantly less than that for 26S rDNA (98.6%). This result demonstrates that beta-tubulin gene sequences provided higher resolution than 26S rDNA sequences. Species-specific restriction profiles of the Saccharomyces strains were obtained by cutting them with the Tsp509I enzyme. Our data indicate that phylogenetic relationships between these strains are best resolved using sequencing or RFLP analysis of the beta-tubulin gene.

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Section: Discussionmentioning

confidence: 99%

The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

Huang

Lee

Tai

2008

Antonie van Leeuwenhoek

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“…A DNA fingerprinting method was developed by Dos Santos et al . () with the purpose to discriminate the seven yeast species ( S. cerevisiae , S. paradoxus , S. bayanus , S. pastorianus, S. cariocanus , S. mikatae and S. kudriavzevii ).…”

Section: Introductionmentioning

confidence: 99%

“…Salinas et al (2009) developed a Taqman real-time PCR assay, based on the amplification of random sequences, for the detection of S. cerevisiae with respect to S. bayanus and S. pastorianus or other non-Saccharomyces yeasts. A DNA fingerprinting method was developed by Dos Santos et al (2007) with the purpose to discriminate the seven yeast species (S. cerevisiae, S. paradoxus, S. bayanus, S. pastorianus, S. cariocanus, S. mikatae and S. kudriavzevii).…”

Section: Introductionmentioning

confidence: 99%

New rapid PCR protocol based on high‐resolution melting analysis to identify Saccharomyces cerevisiae and other species within its genus

et al. 2018

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Current awareness on yeast

2008

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 6th. Feb. 2008)

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Identification of yeasts within Saccharomyces sensu stricto complex by PCR-fingerprinting

Cited by 9 publications

References 28 publications

The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

New rapid PCR protocol based on high‐resolution melting analysis to identify Saccharomyces cerevisiae and other species within its genus

Current awareness on yeast

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