Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Abstract

Heat shock protein (Hsp) gene family members in the watermelon genome were identified and characterized by bioinformatics analysis. In addition, expression profiles of genes under combined drought and heat stress conditions were experimentally analyzed. In the watermelon genome, 39 genes belonging to the sHsp family, 101 genes belonging to the Hsp40 family, 23 genes belonging to the Hsp60 family, 12 genes belonging to the Hsp70 family, 6 genes belonging to the Hsp90 family, and 102 genes belonging to the Hsp100 family were found. It was also observed that the proteins in the same cluster in the phylogenetic trees had similar motif patterns. When the estimated 3-dimensional structures of the Hsp proteins were examined, it was determined that the α-helical structure was dominant in almost all families. The most orthologous relationship appeared to be between watermelon, soybean, and poplar in the ClaHsp gene families. For tissue-specific gene expression analysis under combined stress conditions, expression analysis of one representative Hsp gene each from root, stem, leaf, and shoot tissues was performed by real-time PCR. A significant increase was detected usually at 30 min in almost all tissues. This study provides extensive information for watermelon Hsps, and can enhance our knowledge about the relationships between Hsp genes and combined stresses.

Genome‐wide identification and expression analysis of heat shock protein gene family in cassava

“…Maximum likelihood phylogenetic trees were constructed to analyze the evolutionary relationships of Hsp s between cassava and other species (Table S2). The six Hsp subfamilies were further divided based on the branch status of their phylogenetic trees (Altunoglu et al., 2019). Three main groups (Groups A–C) were identified for both Me Hsp100 and Me Hsp90 proteins (Figure S1a,b).…”

“…Understanding cassava Hsps holds the potential to provide practical approaches for the genetic improvement of cassava. Heat shock proteins have been extensively characterized in many species, with 153 Hsps characterized in Arabidopsis (Arabidopsis thaliana), 72 Hsps in cucumber (Cucumis sativus), 753 Hsps in wheat (Triticum aestivum), and 283 Hsps in watermelon (Citrillus lanatus) (Altunoglu et al, 2019;X. Chen et al, 2021;A.…”

“…Heat shock proteins have been extensively characterized in many species, with 153 Hsp s characterized in Arabidopsis ( Arabidopsis thaliana ), 72 Hsp s in cucumber ( Cucumis sativus ), 753 Hsp s in wheat ( Triticum aestivum ), and 283 Hsp s in watermelon ( Citrillus lanatus ) (Altunoglu et al., 2019; X. Chen et al., 2021; A. Kumar et al., 2020; Swindell et al., 2007). Based on their molecular weight and sequence homology, Hsp s can be further categorized into six subfamilies, that is, Hsp100 , Hsp90 , Hsp70 , Hsp60 , Hsp40 , and small heat shock proteins ( Hsp20 ) (A. Kumar et al., 2020).…”

The New Green Challenge in Urban Planning: The Right Genetics in the Right Place

“…Hence, attention to these possible relations must be paid if the goal is plants' genetic improvement for multiple sources of stress. To date, the genetic bases of water deficiency tolerance have been investigated through genomic, transcriptomic, and metabolomic approaches also in species of the greatest horticultural interest but suitable in urban areas [48,[79][80][81][82], and in sunflower [83][84][85]. This led to the identification of QTLs (Quantitative Trait Loci), single genes, specific sequences such as miRNAs, or genome-wide marker collections potentially related to drought traits.…”