IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS

Abstract

Ugni molinae Turcz. Myrtaceae (Murtilla) is a plant native to Chile. There is evidence about the antiproliferative compounds present in fruits, but there is no evidence on the antiproliferative activity of the compounds in the leaves. These compounds have importance because they predominate in the popular form of ingestion of leaves. In the present study, the identification of water-soluble components of leaves of U. molinae and a fractionation were carried out to determine the effect on viability of gastric adenocarcinoma cells (AGS). A decoct of leaves was performed at 1%. For the separation of fractions, a countercurrent system (CPC) was used. Leaves were collected and identified by thinlayer chromatography and liquid chromatography-mass spectrometry (HPLC MS/MS). Samples were evaluated to determine the viability of AGS cells by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assays. Three large fractions were identified. In the first one, the phenolic compounds of medium polarity and low molecular weight (phenolic acids) are found. In The second fraction shows low concentrations of phenolic compounds with greater molecular weight (glycosylated and gallolylated flavonoids); and the third, has a predominance of hydrolysable tannins, procianidins and other highly hydrophilic compounds. About cell viability, it was found that, the 1% extract is the one that presented the best effects on cell viability inhibition, in comparation to tannin and flavonoid fractions. In conclusion, there is synergy in the compounds present in the aqueous extract, which causes greater inhibition of cell viability, unlike the separated fractions.

Evaluation of the antioxidant, anti-inflammatory, and anti-tumoral properties of bioactive compounds extracted from murta berries (Ugni molinae T.) dried by different methods

“…Braganca De Moraes et al (2012) reported that catechin inhibited the proliferation of GRX liver cells, attributing the action to the inhibition of inflammation and the cell cycle. Additionally, Abd-Rabou et al (2016) found anticarcinogenic ellagic and gallic acids to be cytotoxic mediators against HepG2 and HCT 116 cell lines and decreasing viable human colon tumor cells; Flis et al (2012) showed the effectiveness of antioxidant murta berry extracts-correlated with its phenols-against colon cancer cell lines; Avello et al (2020) found that total aqueous murta leaf extract had synergistic effects in inhibiting cell viability against gastric adenocarcinoma cells (AGS) and that the metabolites of some elements even inhibited the viability of Caco-2 cells.…”

The murta (Ugni molinae) and its beneficial health properties: Areview

“…Los berries han sido catalogados como frutos con importantes propiedades beneficiosas para la salud humana (Avello, Pastene, Bustos, Bittner, & Becerra, 2013;Riquelme et al, 2014), los cuales son asociados a la defensa de diferentes patologías y aplicaciones en el tratamiento y prevención del cáncer. por ejemplo, se ha demostrado que los componentes hidrosolubles de hojas de Ugni molinae tienen efecto sobre la viabilidad de células de cáncer gástrico en humanos (Arancibia-Radich et al, 2016;Avello, Pastene, & Eillen, 2020;Goity et al, 2013); así como las actividades biológicas, antimicrobianas y antioxidantes (López de Dicastillo et al, 2017). En los extractos de semilla de murta se encuentran presentes metabolitos secundarios tales como ácidos fenólicos, flavonoides y taninos, considerados como precursores de defensa contra el ataque de patógenos relacionadas con la salud humana y alimentaria (Cabrera-Barjas et al, 2020).…”