Identification of Volatile Oil Components ofNepeta binaludensisJamzad by GC-MS and¹³C-NMR Methods and Evaluation of its Antimicrobial Activity

Abstract

Abstract:The essential oil of the aerial parts of Nepeta binaludensis Jamzad , an endemic species to Iran, was obtained by hydro-distillation and analyzed by GC, GC-MS and then 13 C-NMR spectra of the main compounds. Sixty-five components, representing 97.42 % of the oil were identified. The major components of the oil were 1,8-cineol (68.31 %), α-terpineol (5.24 %), β-pinene (4.74 %), δ-terpineol (2.57 %), α-pinene (1.54 %). The minimum inhibitory concentrations (MICs) and minimum cidal concentrations (MCCs) of the essential oil and it's major component, 1,8-cineol, as authentic compound were determined using broth dilution method against four bacteria and one fungus. The essential oil was moderately active against Bacillus cereus (3.125 mg/ml), Escherichia coli (3.125 mg/ml), Staphylococcus aureus (6.25 mg/ml) and Candida albicans (12.5 mg/ml) with the same MIC and MCC values in each case. Another gram negative bacteria, Pseudomonas aeroginosa, appeard not to be susceptible to inhibitory effects of the essential oil. The obtained MIC and MCC values for 1,8-cineol were closely near to the essential oil values.

Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.

“…In Adiguzel et al (2009) research, was studied the essential oil of Nepeta cataria and it was identified 22 compounds, dominantly 4aα,7α,7aβ-nepetalactone (70.4%), 4aα,7α,7aα-nepetalactone (6.0%) and 4aβ,7α,7aβ-nepetalactone (2.5%). In similar study was detected that essential oil of N. binaludensis contained 4aα, 7α, 7aα-nepetalactone (19%) as one of the main compounds (Mohammadpour et al 2013).…”

Essential oil analysis of eight 'Nepeta' taxa in Iran

“…We have shown 1,8-cineol (43.5%), and 4aα,7α,7aβ-nepetalactone (23.5%) were the major components in essential oils of N. binaludensis. Mohammadpour et al (2013) reported that 1,8-cineol (68.3%) and α-terpineol (5.2%) were the major components, whereas Rustaiyan & Nadji (1999) suggested the main parts of the oil were 1,8-cineole (42.3%) and 4aα,7α,7aα-nepetalactone (25.2%).…”

“…Based on the au-thor´s field observations, the habitat of populations in the study of Mohammadpour et al (2013) is more saline than other populations, which could lead to a higher percentage of 1,8-cineol. However, our findings of N. binaludensis oil composition were more similar to Rustaiyan & Nadji (1999) rather than Mohammadpour et al (2013). Three main compounds that constitute more than 71% of the oil were the same with a nearly equal amount.…”

“…Three main compounds that constitute more than 71% of the oil were the same with a nearly equal amount. The populations in our study, as well as in the study of Rustaiyan & Nadji (1999), were harvested in the same month (July), while Mohammadpour et al (2013) harvested the plant samples in August. Moreover, according to Hadi et al (2016), the percentages of nepetalactone isomers were strongly affected by harvest time, ecological, and genetic parameters.…”

Antibacterial and Antifungal Activities of Ocimum Gratissimum L.

“…The increasing ineffectiveness in the antibiotic activities used for controlling plant and animal pathogen [2][3][4] has become a big challenge among researchers, clinicians and policymakers. Antimicrobial synthetic products used in food industries are even not safer as for the human health is concerned, leading to chemical toxicity [5].…”