Essential oil analysis of eight 'Nepeta' taxa in Iran

Talebi, Seyed Mehdi; Nohooji, Majid Ghorbani; Yarmohammadi, Mahboobeh

doi:10.5209/mbot.64567

Cited by 7 publications

(7 citation statements)

References 29 publications

(39 reference statements)

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“…The literature search revealed that some other previously studied Nepeta species’ EOs predominantly contained 1,8‐cineole 47–49 . Among the eight Iranian Nepeta species, N. racemosa contained 1,8‐cineole in an amount of 70.9%, 48 which was similar to the value determined in this study (Table 2).…”

Section: Resultssupporting

confidence: 86%

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Aradski

Pavlović

Janošević

et al. 2023

Phytochemical Analysis

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Introduction:The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.Materials and Methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzymeinhibiting effects were evaluated against four enzymes. Results:The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition. Conclusion:This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.

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Section: Resultssupporting

confidence: 86%

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Aradski

Pavlović

Janošević

et al. 2023

Phytochemical Analysis

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“…However, Ali et al ( 2012 ) found 1,8‐cineole (31.2%), and 4aα,7α,7aα‐nepetalactone (14.5%), (E)‐α‐bisabolene (5.4%), α‐terpineol (5.4%), terpinen‐4‐ol (4.8%), linalool (4.5%), and β‐pinene (3.5%) as the major compounds. Moreover, Talebi et al ( 2020 ) considered 1,8‐cineol (53.9%), 4aα,7α,7aα‐nepetalactone (6.2%), Z ‐α‐bisabolene (5%), linalool (4.1%), and rpinen‐4‐ol (3.8%) as the predominant components of this species. It was reported that some environmental and geographical factors such as soil properties, ozone concentration, temperature, wind exposure, relative humidity, photoperiod, light intensity, and partial CO 2 pressure as well as the genetic structure could significantly affect the secondary metabolites synthesis, and consequently, the chemical composition and yield of Lamiaceae essential oil (Kofidis & Bosabalidis, 2008 ; Layeghhaghighi et al, 2017 ; Talebi et al, 2019 ).…”

Section: Resultsmentioning

confidence: 97%

“…However, Ali et al (2012) found 1,8-cineole (31.2%), and 4aα,7α,7aα-nepetalactone (14.5%), (E)αbisabolene (5.4%), α-terpineol (5.4%), terpinen-4-ol (4.8%), linalool (4.5%), and β-pinene (3.5%) as the major compounds. Moreover, Talebi et al (2020) considered 1,8-cineol (53.9%), 4aα,7α,7aαnepetalactone (6.2%), Zα-bisabolene (5%), linalool (4.1%), and rpinen-4-ol (3.8%) as the predominant components of this species.…”

Section: Chemical Composition Of Nepeta Essential Oilmentioning

confidence: 96%

Comparison of antibacterial and antioxidant potentials of pure and nanoemulsified Nepeta pogonosperma essential oil

Sharifi

2022

Food Science & Nutrition

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The current study aimed to investigate the antiradical and antibacterial potential of pure and its nanoemulsified (NNE) Nepeta pogonosperma essential oil (PNE). Antimicrobial activity of the essential oil against two Gram‐positive (E. faecalis and B. cereus) and two Gram‐negative (M. catarrhalis and K. pneumonia) food‐related pathogens during 60‐day storage was investigated based on disc diffusion, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC). The chemical compounds of Nepeta essential oil were estimated by GC/MS. The physical properties of the nanoemulsion including polydispersity index (PDI), mean particle diameter, and viscosity were also determined. 4aα,7α,7aβ‐Nepetalactone (46.31%), 1,8‐cineole (23.13%), and (Z)‐α‐bisabolene (4.01%) were the main compounds of this essential oil. The Nepeta nanoemulsion had a mean droplet diameter of 254.07 nm, PDI of 0.281, and viscosity of 0.887 cP. NNE had stability for up to 60 days. The PNE showed a higher IC50 value than NNE (p < .05). During storage, the antiradical performance of both PNE and NNE was decreased (p < .05). However, emulsification was successful to control this decreasing trend. E. faecalis was the most susceptible bacteria to PNE and NNE, while the lowest inhibition zone was obtained for K. pneumoniae. At the first time, the antibacterial effect of PNE was more than NNE. However, over time nanoemulsion became more successful in maintaining its antibacterial effect. Overall, the incorporation of Nepeta pogonosperma essential oil into a nanoemulsion system can be a promising system to maintain the bioactivity of the essential oil for a longer time.

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“…The application of salinity stress (in the absence of nanoparticles) also created similar results, as with an increase in salinity stress, the amount of artemisinin decreased. Exogenous factors have strong effect on production of different plant metabolites (Talebi et al, 2019(Talebi et al, , 2020. For example, Aftab et al (2010) reported a synergistic relationship between endogenous H 2 O 2 and artemisinin contents, as both contents increased under low levels of salinity (50 and 100 mM) and decreased thereafter.…”

Section: Discussionmentioning

confidence: 99%

TiO2 nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.

et al. 2022

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Artemisia absinthium L. is an important herb that is widely cultivated in different parts of the world for its medicinal properties. The present study evaluated the effects of four concentrations of nanoparticles treatment (0, 10, 20 and 30 mg L-1) and NaCl salinity stress (0, 50, 100 and 150 mM NaCl) and their interactions with respect to the expression of two key genes, i.e. DBR2 and ADS, in the biosynthesis pathway of artemisinin in A. absinthium. Total RNA was extracted and a relative gene expression analysis was carried out using Real-Time PCR. The amount of artemisinin was also determined by HPLC. All the experiments were performed as factorial in a completely randomized design in three replications. The results revealed that salinity stress and nanoparticles treatment and their interaction affected the expressions of these genes significantly. The highest levels of ADS gene expression were observed in the 30 mg L-1 nanoparticles–treated plants in the presence of 150 mM salinity stress and the lowest levels in the 10 mg L-1 nanoparticles–treated plants under 50 mM salinity stress. The maximum DBR2 gene expression was recorded in the 10 mg L-1 nanoparticles–treated plants in the absence of salinity stress and the minimum expression in the 100 mM salinity-stressed plants in the absence of nanoparticles treatment. Moreover, the smallest amounts of artemisinin were observed in the 150 mM salinity-stressed plants in the absence of nanoparticles and the highest amounts in the 30 mg L-1 nanoparticles–treated plants. The maximum amounts of artemisinin and ADS gene expression were reported from the plants in the same nanoparticles treatment and salinity stress conditions. In this regard, the amount of artemisinin was decreased by half in the plants containing the highest DBR2 gene expression. Meanwhile, no significant correlation was observed between these gene expressions and the artemisinin amount in the other nanoparticles–treated plants under different levels of salinity stress. The biosynthetic pathway of secondary metabolites appears to be very complex and dose not directly dependent on these gene expressions.

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Essential oil analysis of eight 'Nepeta' taxa in Iran

Cited by 7 publications

References 29 publications

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Leaves micromorphology, chemical profile, and bioactivity of in vitro‐propagated Nepeta cyrenaica (Lamiaceae)

Comparison of antibacterial and antioxidant potentials of pure and nanoemulsified Nepeta pogonosperma essential oil

TiO2 nanoparticles and salinity stress in relation to artemisinin production and ADS and DBR2 expression in Artemisia absinthium L.

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