Identification of viral agents associated with diarrhea in young children during a winter season in Beijing, China

Liu, Chunyan; Grillner, Lena; Jönsson, Klas; Linde, Annika; Shen, Kunling; Lindell, Annika Tiveljung; Wirgart, Benita Zweygberg; Johansen, Kari

doi:10.1016/j.jcv.2005.04.007

Cited by 55 publications

(61 citation statements)

References 21 publications

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“…The second most frequent norovirus genotype found in this study was GGII.b. Several studies have reported this genotype as a cause of sporadic and outbreak cases of gastroenteritits in children, and some of these studies suggested that GGIIb was closely associated with infections in children [36][37][38][39][40]. In this study, the highest rates of GGIIb detection were observed in January-February 2002 and the fall and winter of [2003][2004].…”

Section: Discussionmentioning

confidence: 51%

Epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a Parisian hospital, France

Lorrot

Bon

Hajje

et al. 2010

Eur J Clin Microbiol Infect Dis

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International audienceRotavirus is recognised as the most important agent of severe acute gastroenteritis (AGE) in young children. In a 2-year prospective survey, we investigated the epidemiology and clinical features of the viral and bacterial pathogens in children hospitalised for AGE. The study was performed in a Parisian teaching hospital from November 2001 to May 2004. Clinical data were prospectively collected to assess the gastroenteritis severity (20-point Vesikari severity score, the need for intravenous rehydration, duration of hospitalisation). Stools were systematically tested for group A rotavirus, norovirus, astrovirus and adenovirus 40/41, sapovirus and Aichi virus and enteropathogenic bacteria. A total of 457 children (mean age 15.9 months) were enrolled. Viruses were detected in 305 cases (66.7%) and bacteria in 31 cases (6.8%). Rotaviruses were the most frequent pathogen (48.8%), followed by noroviruses (8.3%) and adenoviruses, astroviruses, Aichi viruses and sapoviruses in 3.5%, 1.5%, 0.9% and 0.4%, respectively. Cases of rotavirus gastroenteritis were significantly more severe than those of norovirus with respect to the Vesikari score, duration of hospitalisation and the need for intravenous rehydration. Rotaviruses were the most frequent and most severe cause in children hospitalised for AGE, and noroviruses also account for a large number of cases in this population

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Section: Discussionmentioning

confidence: 51%

Epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a Parisian hospital, France

Lorrot

Bon

Hajje

et al. 2010

Eur J Clin Microbiol Infect Dis

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“…The 50 μl RNA eluates were stored at −80°C until amplification of nucleic acid was performed. Primer characteristics and references for the amplification conditions of different PCR or nested PCR protocols used for nucleic acid detection of rotavirus, astrovirus, and norovirus were all described previously (Liu et al 2006;He et al 2008). Briefly a single-step RT-PCR reaction for viruses was performed with the following reaction condition: 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 0.2 mM dNTPs (Promega, Beijing, China), 200U M-MLV RT (Promega, Beijing, China), 2.5 U Ex-Taq DNA polymerase (TaKaRa, Dalian, China), 20 U RNasin (Promega, Beijing, China), and 1 M each of primers.…”

Section: Virus Concentrationmentioning

confidence: 99%

“…Ingestion of 10-100 viral particles is enough to cause disease (Gutiérrez-Aguirre et al 2009). The reported frequency of infection by RV, astrovirus (AV), and norovirus (NV) was 59%, 8%, and 6%, respectively, during the winter season (from December 2000 to March 2001) in Beijing (Liu et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

Wei

et al. 2011

Environ Monit Assess

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To assess the presence of three gastroenteritis viruses responsible for human acute gastroenteritis in surface water, a 1-year study was carried out in the city of Beijing, China. A total of 108 urban surface water samples were collected from nine collection sites which were defined with a global positioning system in rivers or lakes from September 2006 to August 2007. The water samples were subjected to virus concentration using an HA electronegative filter, followed by polymerase chain reaction (PCR) for rotavirus (RV) astrovirus (AV), and norovirus (NV). It showed that the number of viruses detected in water samples from different sites was variable, totaling 63 virus strains, with rotavirus (48.1%) verified as the most prevalent detected, followed by astrovirus (AV, 5.6%), and norovirus (NV, 4.6%). RV was also quantified by real-time PCR and the concentration of RV ranged from 0 to 18.27 genome copies·L −1 . And the distributions of RV in surface water were abundant in cold weather (from September to February) while less prevailing in warm weather (from March to August). The high detection rate of RV we encountered in this study provided convincing evidence that RV circulated at a certain frequency in the Beijing population. There was no statistically significant correlation between RV levels and both fecal coliform (R 2 =0.02) and Enterococcus faecalis (R 2 =0.02) densities. Our study suggests prolonged virus persistence in aquatic environments and emphasizes the enteric virus group as the most reliable for environmental monitoring.

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“…The reported frequencies of infection by rotavirus, astrovirus, and norovirus were 59%, 8% and 6%, respectively, during the winter season (from December 2000 to March 2001) in Beijing Children's Hospital (Liu et al 2006). The infection was most commonly seen among children o5 years old hospitalized with acute diarrhea ) and 96.8% of children were infected under 3 years of age (Tong et al 2003).…”

Section: Introductionmentioning

confidence: 99%

One-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in Beijing, China and associated health risk assessment

Zhang

et al. 2011

Water Science and Technology

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Identification of viral agents associated with diarrhea in young children during a winter season in Beijing, China

Abstract: Enteric viruses play an important role in pediatric diarrhea during the winter season in China. A combination of microscopic examination of stool samples with specific EIA assays to detect virus antigen in stool specimens may be suitable for routine diagnostics.

Cited by 55 publications

References 21 publications

Epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a Parisian hospital, France

Epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a Parisian hospital, France

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

One-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in Beijing, China and associated health risk assessment

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