One-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in Beijing, China and associated health risk assessment

He, Xiaoqing; Li, Cheng; Zhang, D. Y.; Xie, X. M.; Wang, D. H.; Wang, Z.

doi:10.2166/wst.2011.032

Cited by 27 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection frequencies for rotavirus, astrovirus, and norovirus were 48.1%, 5.6%, and 4.6%, respectively, which were in accordance with the reported frequencies of 32.3%, 6.3%, and 3.1% by rotavirus, astrovirus, and norovirus in sewerage systems (He et al 2011). The present study showed a higher prevalence of rotavirus, astrovirus, and norovirus during the cold seasons, but the difference was statistically significant only for rotavirus.…”

Section: Discussionsupporting

confidence: 88%

“…In Beijing, the treated water, regarded as a second water source, has been used as toilet flushing water, industrial process water, environmental water, garden water, and road washing water (He et al 2008). Sewage treatment and disinfective procedures for wastewater have only a limited effect on a number of viruses, and therefore treated sewage water still contain enteric viruses (Meleg et al 2006;He et al 2011). Because of discharges of treated sewage, pathogenic microorganisms may enter surface waters in Beijing, and therefore viruses are able to contam-inate environmental waters thus preserving a longterm capacity for infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

Wei

et al. 2011

Environ Monit Assess

View full text Add to dashboard Cite

To assess the presence of three gastroenteritis viruses responsible for human acute gastroenteritis in surface water, a 1-year study was carried out in the city of Beijing, China. A total of 108 urban surface water samples were collected from nine collection sites which were defined with a global positioning system in rivers or lakes from September 2006 to August 2007. The water samples were subjected to virus concentration using an HA electronegative filter, followed by polymerase chain reaction (PCR) for rotavirus (RV) astrovirus (AV), and norovirus (NV). It showed that the number of viruses detected in water samples from different sites was variable, totaling 63 virus strains, with rotavirus (48.1%) verified as the most prevalent detected, followed by astrovirus (AV, 5.6%), and norovirus (NV, 4.6%). RV was also quantified by real-time PCR and the concentration of RV ranged from 0 to 18.27 genome copies·L −1 . And the distributions of RV in surface water were abundant in cold weather (from September to February) while less prevailing in warm weather (from March to August). The high detection rate of RV we encountered in this study provided convincing evidence that RV circulated at a certain frequency in the Beijing population. There was no statistically significant correlation between RV levels and both fecal coliform (R 2 =0.02) and Enterococcus faecalis (R 2 =0.02) densities. Our study suggests prolonged virus persistence in aquatic environments and emphasizes the enteric virus group as the most reliable for environmental monitoring.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

Wei

et al. 2011

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…First, the analysis of 100 effluent samples allowed us to highlight the high frequencies of detection and the high loads of HAstV, NoV GI, and NoV GII, which showed an overall consistency with the median viral loads in WWTP effluents observed worldwide, for example, 3.9 ϫ 10 4 copies per liter for HAstV, 7.1 ϫ 10 4 copies per liter for NoV GI, and 5.2 ϫ 10 4 copies per liter for NoV GII in Singapore (9), 2.9 ϫ 10 3 copies per liter for NoV GI and 2.6 ϫ 10 3 copies per liter for NoV GII in Japan (8), between 10 2 and 10 3 copies per liter for NoV GI and between 10 2 and 10 4 copies per liter for NoV GII in New Zealand (24), about 10 5 copies per liter for NoV GI and NoV GII in Norway (25), about 10 5 copies per liter for HAstV, NoV GI, and NoV GII in Germany (26), and about 10 6 copies per liter for NoV GI and NoV GII in France (27). Part of the discrepancy in the detection frequencies could be explained by the differences in the concentration and detection methods and the sample volumes used (10,11,28). Our results showed high frequencies of detection and high viral loads in the WWTP effluents, reflecting the high prevalence and high levels of circulation of human HAstV, NoV GI, and NoV GII in the human population.…”

Section: Discussionmentioning

confidence: 99%

“…A large quantity of viral particles is excreted in the feces of infected people (6,7), and they are finally circulated through the wastewater network (8,9). Viruses are generally not eliminated by wastewater treatment plants (WWTPs), and as a consequence, they can be released into rivers at noticeable levels (10)(11)(12). Consequently, we assume that the viruses in WWTP effluents could represent the viruses circulating not only in environmental waters but also in the human population.…”

mentioning

confidence: 99%

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Prévost

Lucas

Ambert-Balay³

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

cAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.T he epidemiology of viral gastroenteritis is mainly evaluated through the use of clinical data. However, clinical data provide only a partial vision of the gastroenteritis viruses circulating in the human population. Indeed, many viral infections do not require specialized consultations or hospitalizations and so are not directly included in clinical statistics. As a consequence, these data sets offer a limited view of the diversity and occurrence of enteric viruses in the human population.Human astroviruses (HAstVs) and noroviruses (NoVs) are among the main causes of human acute gastroenteritis worldwide (1, 2). HAstVs and NoVs are nonenveloped viruses and have a positive-sense single-stranded RNA. The genome of HAstV is composed of three open reading frames (ORFs), and ORF2, encoding the capsid protein precursor, allows discrimination of eight genotypes of HAstV, HAstV-1 to HAstV-8 (3). The genome of NoV is also composed of three ORFs, and genotyping based on the genetic similarity of ORF2 and ORF3 allows discrimination of 9 genotypes of NoV genogroup I (NoV GI) and 22 genotypes of NoV genogroup II (NoV GII), which are the most common genogroups associated with human infections.HAstVs and NoVs are able to infect i...

show abstract

“…Several studies have reported higher frequencies of RV as compared to NoV in wastewater. Research groups from China, Tunisia and Germany described detection rates of RV ranging from 32% to 90% and NoV from 3% to 31% [26], [27], [28]. In hospital wastewater, Prado and coworkers observed RV in 95% and NoV GII in 28.5% of the samples collected between 2005 and 2008 in Rio de Janeiro, Brazil.…”

Section: Discussionmentioning

confidence: 99%

Low Prevalence of Rotavirus and High Prevalence of Norovirus in Hospital and Community Wastewater after Introduction of Rotavirus Vaccine in Nicaragua

et al. 2011

View full text Add to dashboard Cite

Rotavirus (RV) and norovirus (NoV) are major causes of pediatric diarrhea and are altogether associated with approximately 800,000 deaths in young children every year. In Nicaragua, national RV vaccination program using the pentavalent RV5 vaccine from Merck was implemented in October 2006. To determine whether RV vaccination decreased the overall number of RV infections, we investigated the occurrence of RV and NoV in wastewater in the city of León from July 2007 to July 2008 and compared these data with pre-vaccination data. The major finding was the low prevalence of RV compared to NoV in all sampling points (11% vs 44%, p<0.05), and that RV concentration was lower as compared to NoV. RV was observed mainly during the rainy season (July–September), and the majority of all RV detected (6/9) belonged to subgroup (SG) I. The partial VP7-gene obtained from one RV positive sample was similar (99% nt identity) to a G6 VP7-gene of bovine origin and similar to the corresponding gene of the vaccine strain (98%). Furthermore RV G-types 2 and 4 were found in the incoming wastewater. NoV strains were detected throughout the year, of which a majority (20/21) were of genotype GII.4. We conclude that the introduction of RV vaccination reduced the transmission of RV in the community in Nicaragua. However, the burden of diarrhea in the country remains high, and the high prevalence of NoVs in hospital and municipal wastewater is noteworthy. This study highlights the need for further assessment of NoV following RV vaccine introduction.

show abstract

One-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in Beijing, China and associated health risk assessment

Abstract: To evaluate the presence and distribution of the three main viruses (rotavirus, astrovirus, and norovirus) responsible for human acute gastroenteritis in sewerage system an one-year study was

Cited by 27 publications

References 35 publications

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

Molecular detection of three gastroenteritis viruses in urban surface waters in Beijing and correlation with levels of fecal indicator bacteria

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Low Prevalence of Rotavirus and High Prevalence of Norovirus in Hospital and Community Wastewater after Introduction of Rotavirus Vaccine in Nicaragua

Contact Info

Product

Resources

About