Identification of vasculature‐specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos

Wong, Kuan Shen; Proulx, Kira; Rost, Megan S.; Sumanas, Saulius

doi:10.1002/dvdy.21990

Cited by 71 publications

(82 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The zebrafish genome encodes two paralogs of Esam, esama and esamb. In zebrafish, esama expression has been reported in ECs, the endocardium and also in a subset of neurons in the brain, including the epiphysis/pineal gland (Wong et al, 2009). We confirmed the endothelium-specific expression of esama, whereas we observed only weak expression of esamb in the vasculature (Fig.…”

Section: Esama Is Expressed In the Endothelium And Sprouting Seassupporting

confidence: 83%

Distinct and redundant functions of Esama and VE-cadherin during vascular morphogenesis

2017

View full text Add to dashboard Cite

The cardiovascular system forms during early embryogenesis and adapts to embryonic growth by sprouting angiogenesis and vascular remodeling. These processes require fine-tuning of cell-cell adhesion to maintain and re-establish endothelial contacts, while allowing cell motility. We have compared the contribution of two endothelial cellspecific adhesion proteins, VE-cadherin (VE-cad/Cdh5) and Esama (endothelial cell-selective adhesion molecule a), during angiogenic sprouting and blood vessel fusion (anastomosis) in the zebrafish embryo by genetic analyses. Different combinations of mutant alleles can be placed into a phenotypic series with increasing defects in filopodial contact formation. Contact formation in esama mutants appears similar to wild type, whereas esama −/− ; ve-cad +/− and ve-cad single mutants exhibit intermediate phenotypes. The lack of both proteins interrupts filopodial interaction completely. Furthermore, double mutants do not form a stable endothelial monolayer, and display intrajunctional gaps, dislocalization of Zo-1 and defects in apical-basal polarization. In summary, VE-cadherin and Esama have distinct and redundant functions during blood vessel morphogenesis, and both adhesion proteins are central to endothelial cell recognition during anastomosis.

show abstract

Section: Esama Is Expressed In the Endothelium And Sprouting Seassupporting

confidence: 83%

Distinct and redundant functions of Esama and VE-cadherin during vascular morphogenesis

2017

View full text Add to dashboard Cite

show abstract

“…A second receptor [the ANG II type 2 (AT 2 ) receptor], proposed to modulate the actions of the AT 1 receptor, is found in abundance in early fetal development, but expression falls after birth. Similarly, expression of the zebrafish AT 2 receptor (agtr2), seen principally in the DA at 24 hpf, begins to diminish by 36 hpf (78). As expression of renin continues to increase after 36 hpf, the principal roles of renin/RAS in developing zebrafish, be it for ion homeostasis or other functions, may be via the AT 1 rather than AT 2 receptor.…”

Section: Discussionmentioning

confidence: 99%

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

Rider

Mullins

Verdon

et al. 2015

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

Rider SA, Mullins LJ, Verdon RF, MacRae CA, Mullins JJ. Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium. Am J Physiol Renal Physiol 309: F531-F539, 2015. First published July 22, 2015 doi:10.1152/ajprenal.00247.2015.-Although renin is a critical regulatory enzyme of the cardiovascular system, its roles in organogenesis and the establishment of cardiovascular homeostasis remain unclear. Mammalian renin-expressing cells are widespread in embryonic kidneys but are highly restricted, specialized endocrine cells in adults. With a functional pronephros, embryonic zebrafish are ideal for delineating the developmental functions of renin-expressing cells and the mechanisms governing renin transcription. Larval zebrafish renin expression originates in the mural cells of the juxtaglomerular anterior mesenteric artery and subsequently at extrarenal sites. The role of renin was determined by assessing responses to renin-angiotensin system blockade, salinity variation, and renal perfusion ablation. Renin expression did not respond to renal flow ablation but was modulated by inhibition of angiotensin-converting enzyme and altered salinity. Our data in larval fish are consistent with conservation of renin's physiological functions. Using transgenic renin reporter fish, with mindbomb and cloche mutants, we show that Notch signaling and the endothelium are essential for developmental renin expression. After inhibition of angiogenesis, renin-expressing cells precede angiogenic sprouts. Arising from separate lineages, but relying on mutual interplay with endothelial cells, renin-expressing cells are among the earliest mural cells observed in larval fish, performing both endocrine and paracrine functions. renin; zebrafish; notch; endothelium; angiogenesis RENIN is the rate-limiting enzyme of the renin-angiotensin system (RAS). Mammalian ANG II, the effector of the RAS, is principally involved in blood pressure homeostasis by regulation of vasomotor tone and Na ϩ retention. ANG II also regulates cell proliferation and angiogenesis (12,22). Pathological activation of the RAS is associated with cardiovascular diseases, including hypertension and heart failure, and is consequently a major target of therapeutic agents (11).Renin and its cognate cells are required for normal renal development (2), including angiogenesis of the renal vascular tree (59). In mice, ablation of the renin gene or renin-expressing cells leads to renal developmental abnormalities (55,71,79). The vasculature of mammalian embryonic kidneys have a transient but extensive coverage of mural renin-expressing cells (17,31,32,47,63); however, their function is unclear. Adult renin-expressing cells are restricted to the juxtaglomerular apparatus and play a vital role in ion homeostasis by secreting activated renin enzyme via cytoplasmic granules (17,31,36,63). Upon physiological challenge by RAS inhibition or low Na ϩ , prearteriolar vascular smooth muscle cells (VSMC) reestablish their embryonic re...

show abstract

“…Their model fits well with our current results in zebrafish. It is well known that Etv2 overexpression also results in endothelialization in zebrafish (35,37,42,59) and Xenopus (57). All of these observations suggest that Etv2 may play a conserved role in maintaining the endothelial characteristics of hemangioblasts in the early developmental periods.…”

Section: Discussionmentioning

confidence: 99%

“…S5B). Because etv2 has been shown to regulate the expression of endothelial genes positively (35)(36)(37), the expression of fli1a and other endothelial markers (flk1 and tie1) was analyzed at later developmental stages ( Fig. 3 B and C and Fig.…”

mentioning

confidence: 99%

LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2

Takeuchi

Fuse

Watanabe

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The hemangioblast is a progenitor cell with the capacity to give rise to both hematopoietic and endothelial progenitors. Currently, the regulatory mechanisms underlying hemangioblast formation are being elucidated, whereas those controllers for the selection of hematopoietic or endothelial fates still remain a mystery. To answer these questions, we screened for zebrafish mutants that have defects in the hemangioblast expression of Gata1, which is never expressed in endothelial progenitors. One of the isolated mutants, it627, showed not only down-regulation of hematopoietic genes but also up-regulation of endothelial genes. We identified the gene responsible for the it627 mutant as the zebrafish homolog of Lys-specific demethylase 1 (LSD1/KDM1A). Surprisingly, the hematopoietic defects in lsd1it627 embryos were rescued by the gene knockdown of the Ets variant 2 gene (etv2), an essential regulator for vasculogenesis. Our results suggest that the LSD1-dependent shutdown of Etv2 gene expression may be a significant event required for hemangioblasts to initiate hematopoietic differentiation.

show abstract

Identification of vasculature‐specific genes by microarray analysis of etsrp/etv2 overexpressing zebrafish embryos

Cited by 71 publications

References 71 publications

Distinct and redundant functions of Esama and VE-cadherin during vascular morphogenesis

Distinct and redundant functions of Esama and VE-cadherin during vascular morphogenesis

Renin expression in developing zebrafish is associated with angiogenesis and requires the Notch pathway and endothelium

LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2

Contact Info

Product

Resources

About