Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (<i>Camellia sinensis</i>)

Cui, Lilan; Yao, Shengbo; Dai, Xinlong; Yin, Qinggang; Liu, Yajun; Jiang, Xiaolan; Wu, Yahui; Qian, Yumei; Pang, Yongzhen; Gao, Liping; Xia, Tao

doi:10.1093/jxb/erw053

Cited by 167 publications

(210 citation statements)

References 44 publications

(66 reference statements)

Supporting

Mentioning

204

Contrasting

Order By: Relevance

“…S2). In Arabidopsis , the UGTs that displayed catalytic activity in vitro towards terpenoids were restricted to groups D, E, G, H and L of the phylogenetic tree of the GT1 multigene family (Caputi et al , ; Cui et al , ). In the tea plant, some UGTs belonging to the subgroups A (UGT94P1) and G (UGT85K11 and UGT85A53) showed broad substrate specificity towards monoterpenes (Jing et al , ; Ohgami et al , ).…”

Section: Resultsmentioning

confidence: 99%

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

et al. 2020

View full text Add to dashboard Cite

Summary Plants produce and emit terpenes, including sesquiterpenes, during growth and development, which serve different functions in plants. The sesquiterpene nerolidol has health‐promoting properties and adds a floral scent to plants. However, the glycosylation mechanism of nerolidol and its biological roles in plants remained unknown. Sesquiterpene UDP‐glucosyltransferases were selected by using metabolites‐genes correlation analysis, and its roles in response to cold stress were studied. We discovered the first plant UGT (UGT91Q2) in tea plant, whose expression is strongly induced by cold stress and which specifically catalyzes the glucosylation of nerolidol. The accumulation of nerolidol glucoside was consistent with the expression level of UGT91Q2 in response to cold stress, as well as in different tea cultivars. The reactive oxygen species (ROS) scavenging capacity of nerolidol glucoside was significantly higher than that of free nerolidol. Down‐regulation of UGT91Q2 resulted in reduced accumulation of nerolidol glucoside, ROS scavenging capacity and tea plant cold tolerance. Tea plants absorbed airborne nerolidol and converted it to its glucoside, subsequently enhancing tea plant cold stress tolerance. Nerolidol plays a role in response to cold stress as well as in triggering plant–plant communication in response to cold stress. Our findings reveal previously unidentified roles of volatiles in response to abiotic stress in plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This hypothesis is based on our previous observation during the isolation of galloyltransferase from tea leaves, which was characterized to catalyze the formation of galloylated flavan‐3‐ol in tea leaves (Fig. b; Liu et al , ; Cui et al , ). During the isolation of galloyltransferase from tea leaves, we observed that TA‐like catalysis always occurred.…”

Section: Introductionmentioning

confidence: 95%

Discovery and characterization of tannase genes in plants: roles in hydrolysis of tannins

Dai

Zhuang

et al. 2020

New Phytologist

Self Cite

View full text Add to dashboard Cite

Plant tannins, including condensed tannins (CTs) and hydrolyzable tannins (HTs), are widely distributed in the plant kingdom. To date, tannase (TA)is a type of tannin acyl-hydrolase hydrolyzing HTs, CT monomer gallates and depsideshas been reported in microbes only. Whether plants express TA remains unknown.Herein, we report plant TA genes. A native Camellia sinensis TA (CsTA) is identified from leaves. Six TAs are cloned from tea, strawberry (Fragaria 9 ananassa, Fa) and four other crops. Biochemical analysis shows that the native CsTA and six recombinant TAs hydrolyze tannin compounds, depsides and phenolic glycosides.Transcriptional and metabolic analyses reveal that the expression of CsTA is oppositely associated with the accumulation of galloylated catechins. Moreover, the transient overexpression and RNA interference of FaTA are positively associated with the accumulation of ellagitannins in strawberry fruit. Phylogenetic analysis across different kingdoms shows that 29 plant TA homologs are clustered as a plant-specific TA clade in class I carboxylesterases. Further analysis across the angiosperms reveals that these TA genes are dispersed in tanninrich plants, which share a single phylogenetic origin c. 120 million yr ago.Plant TA is discovered for the first time in the plant kingdom and is shown to be valuable to improve tannin compositions in plants.

show abstract

“…RNA-Sequencing data showed that several CsUGTs, especially CsUGT72AM1 and CsUGT3 , were remarkably up-regulated in ‘Mooma1’ leaf, compared with the value in wild-type tea. Our previous research had identified a UDP-glycosyltransferase, CsUGT78A14 , which is responsible for the biosynthesis of flavonol 3- O -glucosides (Cui et al ., 2016). The expression of CsUGT78A14 was unremarkably up-regulated in the ‘Mooma1’ leaf compared with wild-type control.…”

Section: Resultsmentioning

confidence: 99%

“…78 UGT family genes, such as UGT78G1 (Modolo et al ., 2009), UGT78D2 (Tohge et al ., 2005) were activated with anthocyanidins, flavonols, flavones, coumestans, pterocarpans, and isoflavones, and were involved in the O-glucosylation of anthocyanins. In our previous study, CsUGT78A14 was found to be responsible for the biosynthesis of flavonol 3-O-glucosides (Cui et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Recombinant proteins were purified according to the manufacturer’s instructions (New England Biolabs, MA, USA). Recombinant enzyme assays were carried out as described by Cui et al (Cui et al ., 2016). The K m and V max of CsUGT72AM1 was determined using 5 mM UDP-glucose (UDP-Glc) as the sugar donor and 1.5–200 μM of flavonols as acceptors (kaempferol and quercetin) in phosphate buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of key genes that promote flavonoid accumulation in purple-leaf tea (Camellia sinensisL.)

Zhao

Gao

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

There were several high concentrations of flavonoid components in tea leaves that present health benefits. A novel purple-leaf tea variety, 'Mooma1', was obtained from the natural hybrid population of Longjing 43 variety. The buds and young leaves of 'Mooma1' were displayed in bright red. HPLC and LC-MS analysis showed that anthocyanins and O-Glycosylated flavonols were remarkably accumulated in the leaves of 'Mooma1', while the total amount of catechins in purple-leaf leaves was slightly decreased compared with the control. A R2R3-MYB transcription factor (CsMYB6A) and a novel UGT gene (CsUGT72AM1), that were highly expressed in purple leaf were isolated and identified by transcriptome sequencing. The over-expression of transgenic tobacco confirmed that CsMYB6A can activate the expression of flavonoid-related structural genes, especially CHS and 3GT, controlling the accumulation of anthocyanins in the leaf of transgenic tobacco. Enzymatic assays in vitro confirmed that CsUGT72AM1 has catalytic activity as a flavonol 3-O-glucosyltransferase, and displayed broad substrate specificity. The results were useful for further elucidating the molecular mechanisms of the flavonoid metabolic fluxes in the tea plant.

show abstract

Identification of UDP-glycosyltransferases involved in the biosynthesis of astringent taste compounds in tea (Camellia sinensis)

Abstract: HighlightThe identification of three UDP-glycosyltransferases involved in the biosynthesis of galloylated catechins and glycosylated flavonols which are astringent taste compounds in tea.

Cited by 167 publications

References 44 publications

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants

Discovery and characterization of tannase genes in plants: roles in hydrolysis of tannins

Isolation and characterization of key genes that promote flavonoid accumulation in purple-leaf tea (Camellia sinensisL.)

Contact Info

Product

Resources

About