Identification of two novel Rotavirus A genotypes, G35 and P[50], from Peruvian alpaca faeces

Rojas, Miguel; Gonçalves, Jorge Luiz S.; Dias, Helver Gonçalves; Manchego, S Alberto; Santos, Norma

doi:10.1016/j.meegid.2017.08.019

Cited by 20 publications

(16 citation statements)

References 13 publications

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“…On the other hand, it showed 92.7% nucleotide identity with a bat strain (KCR10‐93) detected in Costa Rica, which has 80% nucleotide identity with the Ecu534 strain. The sequence was sent to the (RCWG [Rotavirus Classification Working Group, ]), which classified this strain as the prototype of the new G35 genotype (Rojas, Gonçalves, Dias, Manchego, & Santos, ). Genotypes G1 (alpaca, llama and sheep), G3 (alpaca, llama and human), G8 (alpaca) and G35 (alpaca) were identified.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity and zoonotic potential of rotavirus A strains in the southern Andean highlands, Peru

Rojas

Dias

Gonçalves

et al. 2019

Transbound Emerg Dis

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Interspecies transmission is an important mechanism of evolution and contributes to rotavirus A (RVA) diversity. In order to evaluate the detection frequency, genetic diversity, epidemiological characteristics and zoonotic potential of RVA strains in faecal specimens from humans and animals cohabiting in the same environment in the department of Cusco, Peru, by molecular analysis, 265 faecal specimens were obtained from alpacas, llamas, sheep and shepherd children, and tested for RVA by RT‐PCR. Genotyping was performed by multiplex PCR and sequence analysis. Rotavirus A was detected in 20.3% of alpaca, 47.5% of llama, 100% of sheep and 33.3% of human samples. The most common genetic constellations were G3‐P[40]‐I8‐E3‐H6 in alpacas, G1/G3‐P[8]‐I1‐E1‐H1 in llamas, G1/G3/G35‐P[1]/P[8]‐I1‐E1‐H1 in sheep and G3‐P[40]‐I1/I8‐E3‐H1 in humans. The newly described genotypes P[40] and P[50] were identified in all host species, including humans. Genotyping showed that the majority of samples presented coinfection with two or more RVA strains. These data demonstrate the great genetic diversity of RVA in animals and humans in Cusco, Peru. Phylogenetic analysis suggested that the strains represent zoonotic transmission among the species studied. Due to the characteristics of the human and animal populations in this study (cohabitation of different host species in conditions of poor sanitation and hygiene), the occurrence of zoonoses is a real possibility.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity and zoonotic potential of rotavirus A strains in the southern Andean highlands, Peru

Rojas

Dias

Gonçalves

et al. 2019

Transbound Emerg Dis

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“…RVA belongs to the Reoviridae family and is a non-enveloped virus, consisting of 11 segments of double-stranded RNA and encoding six structural and six non-structural proteins (NSPs). RVA is classified into 35 G and 50 P types on the basis of VP7 (glycoprotein) and VP4 (protease-sensitive) protein (Rojas et al, 2017), respectively. RVA genotypes G1P[8], G2P[4], G3P[8], G4P[8], G9P[8], and G12P[8] are commonly found worldwide (Santos and Hoshino, 2005; Dóró et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology and Clinical Features of Rotavirus Infection Among Pediatric Patients in East Java, Indonesia During 2015–2018: Dynamic Changes in Rotavirus Genotypes From Equine-Like G3 to Typical Human G1/G3

et al. 2019

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Group A rotavirus (RVA) is the most important cause of severe gastroenteritis among children worldwide, and effective RVA vaccines have been introduced in many countries. Here we performed a molecular epidemiological analysis of RVA infection among pediatric patients in East Java, Indonesia, during 2015-2018. A total of 432 stool samples were collected from hospitalized pediatric patients with acute gastroenteritis. None of the patients in this cohort had been immunized with an RVA vaccine. The overall prevalence of RVA infection was 31.7% (137/432), and RVA infection was significantly more prevalent in the 6-to 11-month age group than in the other age groups (P < 0.05). Multiplex reverse transcription-PCR (RT-PCR) revealed that the most common G-P combination was equine-like G3P[8] (70.8%), followed by equine-like G3P[6] (12.4%), human G1P[8] (8.8%), G3P[6] (1.5%), and G1P[6] (0.7%). Interestingly, the equine-like strains were exclusively detected until May 2017, but in July 2017 they were completely replaced by a typical human genotype (G1 and G3), suggesting that the dynamic changes in RVA genotypes from equine-like G3 to typical human G1/G3 in Indonesia can occur even in the country with low RVA vaccine coverage rate. The mechanism of the dynamic changes in RVA genotypes needs to be explored. Infants and children with RVA-associated gastroenteritis presented more frequently with some dehydration, vomiting, and watery diarrhea, indicating a greater severity of RVA infection compared to those with non-RVA gastroenteritis. In conclusion, a dynamic change was found

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“…RVAs are further classified into G genotypes and P genotypes on the basis of the nucleotide sequence of VP7 (glycoprotein) and VP4 (protease‐sensitive) genes, which support a widely used binary classification system. Currently, 35G genotypes and 50P genotypes of RVAs have been described …”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of human G9P[8] rotavirus strains circulating in Jiangsu, China between 2010 and 2016

et al. 2018

Journal of Medical Virology

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Rotavirus A (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years of age worldwide. G9P[8] is a common RVA genotype that has been persistently prevalent in Jiangsu, China. To determine the genetic diversity of G9P[8] RVAs, 7 representative G9P[8] strains collected from Suzhou Children's Hospital between 2010 and 2016 (named JS2010-JS2016) were analyzed through whole-genome sequencing. All evaluated strains showed the Wa-like constellation G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Furthermore, phylogenetic analysis revealed that the VP7 genes of all strains clustered into lineage G9-III and G9-VI. With the exception of strain JS2012 (P[8]-4), the VP4 sequences of all strains belonged to the P[8]-3 lineage. Sequencing further revealed that amino acid substitutions were present in the antigenic regions of the VP7 and VP4 genes of all strains. Moreover, there were multiple substitutions in antigenic sites I and II of the nonstructural protein 4 (NSP4) genes, whereas the other NSP genes were relatively conserved. In conclusion, our phylogenetic analysis of these 7 G9P[8] strains suggests that RVA varied across regions and time. Therefore, our findings suggest that continued surveillance is necessary to explore the molecular evolutionary characteristics of RVA for better prevention and treatment of acute viral gastroenteritis.

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Identification of two novel Rotavirus A genotypes, G35 and P[50], from Peruvian alpaca faeces

Abstract: Rotavirus A (RVA) Alp11B was detected from a neonatal Peruvian alpaca presenting with diarrhea, and the Alp11B VP7, VP4, VP6, NSP4, and NSP5 genes were sequenced. The partial genotype constellation of this strain, RVA/Alpaca-wt/PER/Alp11B/2010, was determined to be G35-P[50]-I13-E16-H6.

Cited by 20 publications

References 13 publications

Genetic diversity and zoonotic potential of rotavirus A strains in the southern Andean highlands, Peru

Genetic diversity and zoonotic potential of rotavirus A strains in the southern Andean highlands, Peru

Molecular Epidemiology and Clinical Features of Rotavirus Infection Among Pediatric Patients in East Java, Indonesia During 2015–2018: Dynamic Changes in Rotavirus Genotypes From Equine-Like G3 to Typical Human G1/G3

Phylogenetic analysis of human G9P[8] rotavirus strains circulating in Jiangsu, China between 2010 and 2016

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