Identification of two hsp90 genes in carp

Hermesz, Edit; Ábrahám, Magdolna; Nemcsók, J.

doi:10.1016/s1532-0456(01)00216-2

Cited by 35 publications

(27 citation statements)

References 30 publications

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“…We found that TaHSP90b is expressed at a relatively high level in all the organs examined, suggesting that TaHSP90 was intensively distributed. This was similar to the findings in other fish such as Psetta maxima (Wang et al 2010) and carp (Hermesz et al 2001). However, individual differences in expression were also observed.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, HSP90 is involved in protein folding, cytoprotection and proteosomic degradation and teams up with a large set of co-chaperones to mediate the conformational regulation of several client proteins (Picard 2006;Hartl and Hayer-Hart 2002;Zhang and Burrows 2004;Brown et al 2007). HSP90 has potential as a new biomarker in environment because it can be triggered by a range of stressful conditions such as high temperature and humidity (Palmisano et al 2000;Gibbs et al 2003;Sonoda et al 2006), hyperosmotic stress (Pan et al 2000), food deprivation (Cara et al 2005), oxidative stress (Gophna and Ron 2003), polychlorinated biphenyl (PCB) (Silke and Bernd 2003), arsenates (Somji et al 2002) and the presence of heavy metals (Ali et al 1996;Papaconstantinou et al 2003;Dundjerski et al 2000;Hermesz et al 2001;Tomanek and Somero 2002;Gao et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Liu

Chen

Jing

et al. 2011

Fish Physiol Biochem

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Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of proteins. In order to evaluate the effect of copper (Cu(2+)) and cadmium (Cd(2+)) on the expression of HSP90 from Tanichthys albonubes (designated TaHSP90), the full-length complementary DNA (cDNA) of TaHSP90 was cloned using reverse transcription PCR and rapid amplification of cDNA ends (RACE) techniques. A 2,687-bp sequence was sequenced and consisted of an open reading frame (ORF) of 2,181 bp encoding a polypeptide of 727 amino acids with five HSP90 family signatures. Homologous analysis revealed that TaHSP90 gene shared high similarity with other known HSP90 genes and belonged to HSP90β subtype. Fluorescent real-time quantitative PCR was used to examine the expression pattern of TaHSP90β mRNA in different tissues (liver, muscle, gill, fin, eye, ovary, intestine and brain), and the result indicated that TaHSP90β was widely expressed in all examined tissues at different levels. Sensitivity of TaHSP90β to copper and cadmium was examined by exposing fish to different concentrations of Cu(2+) (0, 13.50 and 27.00 μg/L) and Cd(2+) (0, 1.15, 2.31 mg/L) for 24, 48, 72 and 96 h, respectively. The copper treatment induced TaHSP90β expression slight increase only at 24 and 48 h, while cadmium treatment caused slight down-regulation of TaHSP90β only 72 and 96 h. Our data suggest that the cloning and expression analysis of T. albonubes HSP90β gene provided useful molecular information of T. albonubes responses in stress conditions and potential ways to monitor the chronic stressors in T. albonubes culture environments.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Liu

Chen

Jing

et al. 2011

Fish Physiol Biochem

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“…Hence, the HSP90 family provides a useful model system for evolutionary studies (Gupta 1995). In addition, the response of these proteins to environmental stresses, such as changes in temperature (Palmisano et al 2000;Hermesz et al 2001;Landais et al 2001), hyperosmotic shock (Pan et al 2000), hypoxia (Li et al 2008), metal ions (Ali et al 1996), polychlorinated biphenyls (Wiens et al 2000), and also diseases (Ramaglia et al 2004) has been examined. Despite of these various studies, only five complete cDNA sequences have been reported from mollusks (Farcy et al 2007;Gunter and Degnan 2007), including three HSP90 cDNA sequences in bivalves (Gao et al 2007(Gao et al , 2008Choi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

Kim

Ahn

Kim

et al. 2009

Cell Stress and Chaperones

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Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone that plays a key role in protein synthesis, folding, denaturation prevention, and signal transduction. We cloned the complete complementary DNA (cDNA) sequence of the Laternula elliptica HSP90. The full-length cDNA was 2,823 bp in size and contained an open reading frame of 2,190 bp that was translated into 729 amino acids with a calculated molecular weight of 83.4 kDa. The deduced amino acid sequence of HSP90 showed the highest homology to Haliotis tuberculata HSP90 (83%). Reverse-transcriptase polymerase chain reaction analysis revealed the presence of HSP90 transcripts in all of the tissues examined. We also studied the transcriptional expression pattern of HSP90 exposed to thermal stress with real-time polymerase chain reaction. The relative expression level of HSP90 messenger RNA was upregulated and peaked at 12 h in the digestive gland and at 24 h in the gills, then dropped progressively.

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“…One possible explanation for this observation may be that the fish had not yet been fully acclimated to the colder temperature. Typical acclimation periods used in various studies on fish thermal acclimation are three weeks (Ali et al, 2003;Cossins et al, 1977;Currie et al, 1998;Hermesz et al, 2001;Viant et al, 2003) to one month (Currie et al, 1999;Sollid et al, 2005). Therefore, we selected an acclimation time of at least four weeks.…”

Section: Effects Of Acclimationmentioning

confidence: 99%

The influence of ambient temperature and thermal acclimation on hearing in a eurythermal and a stenothermal otophysan fish

Wysocki

Montey

Popper

2009

Journal of Experimental Biology

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SUMMARY Being ectothermic, fish body temperature generally depends on ambient water temperature. Thus, ambient temperature might affect various sensory systems,including hearing, as a result of metabolic and physiological processes. However, the maintenance of sensory functions in a changing environment may be crucial for an animal's survival. Many fish species rely on hearing for acoustic orientation and communication. In order to investigate the influence of temperature on the auditory system, channel catfish Ictalurus punctatus was chosen as a model for a eurytherm species and the tropical catfish Pimelodus pictus as a model for a stenotherm fish. Hearing sensitivity was measured with animals acclimated or unacclimated to different water temperatures. Ambient water temperature significantly influenced hearing thresholds and the shape of auditory evoked potentials, especially at higher frequencies in I. punctatus. Hearing sensitivity of I. punctatus was lowest at 10°C and increased by up to 36 dB between 10°C and 26°C. Significant differences were also revealed between acclimated and unacclimated animals after an increase in water temperature but not a decrease. By contrast, differences in hearing thresholds were smaller in P. pictus, even if a similar temperature difference (8°C) was considered. However, P. pictus showed a similar trend as I. punctatus in exhibiting higher hearing sensitivity at the highest tested temperature, especially at the highest frequency tested. The results therefore suggest that the functional temperature dependence of sensory systems may differ depending upon whether a species is physiologically adapted to tolerate a wide or narrow temperature range.

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Identification of two hsp90 genes in carp

Cited by 35 publications

References 30 publications

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

The influence of ambient temperature and thermal acclimation on hearing in a eurythermal and a stenothermal otophysan fish

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