Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

Kim, Meesun; Ahn, In-Young; Kim, Hakjun; Cheon, Jina; Park, Hyun

doi:10.1007/s12192-008-0090-9

Cited by 25 publications

(13 citation statements)

References 39 publications

(46 reference statements)

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“…The expression of Hsp90 under thermal stress in S. neumayeri was similar in the two tissues analyzed, showing a tendency to increase its expression at 3°C at 48 h, but not at a higher temperature (5°C). This situation contrasts with that observed in the Antarctic clam Laternula elliptica where the relative expression level of Hsp90 messenger RNA at 10°C was clearly upregulated and peaked at 12 h in the digestive gland and at 24 h in the gills, then dropped progressively [45], evidencing a faster HSP response in this mollusc compared to the sea urchin S. neumayeri.…”

Section: Discussioncontrasting

confidence: 95%

Expression pattern of heat shock proteins during acute thermal stress in the Antarctic sea urchin, Sterechinus neumayeri

González

Gaitán‐Espitía

Font

et al. 2016

Rev. Chil. de Hist. Nat.

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Background: Antarctic marine organisms have evolved a variety of physiological, life-history and molecular adaptations that allow them to cope with the extreme conditions in one of the coldest and most temperaturestable marine environments on Earth. The increase in temperature of the Southern Ocean, product of climate change, represents a great challenge for the survival of these organisms. It has been documented that some Antarctic marine invertebrates are not capable of generating a thermal stress response by means of an increase in the synthesis of heat shock proteins, which could be related with their low capacity for acclimatization. In order to understand the role of heat shock proteins as a compensatory response in Antarctic marine species to projected scenarios of increased seawater temperatures, we assessed the expression of the genes Hsp90, Grp78, Hyou1 and Hsc70 in the Antarctic sea urchin Sterechinus neumayeri under three thermal treatments (1°C, 3°C and 5°C), for a period of exposure of 1, 24 and 48 h. Results: The results obtained showed that these genes were expressed themselves in all of the tissues analyzed in a constitutive form. During acute thermal stress, an overexpression of the Hsp90, Grp78 and Hyou1 genes was observed in coelomocyte samples at 3°C after 48 h, while in esophageal samples, an increase in Hsp90 and Grp78 expression was observed after 48 h. Thermal stress at 5°C, in general, did not produce a significant increase in the expression of the genes that were studied. The expression of Hsp70 did not show modifications in its expression as a result of thermal stress. Conclusions: S. neumayeri is capable of overexpressing stress proteins as a result of thermal stress, however, this response is delayed and to a lesser degree compared to other Antarctic or temperate species. These results indicate that adult individuals could cope with the expected impacts caused by an increase in coastal sea temperatures in the Southern Ocean.

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Section: Discussioncontrasting

confidence: 95%

Expression pattern of heat shock proteins during acute thermal stress in the Antarctic sea urchin, Sterechinus neumayeri

González

Gaitán‐Espitía

Font

et al. 2016

Rev. Chil. de Hist. Nat.

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“…A significant increase (P < 0.05) in the expression levels of CnHSP90 was observed in both golden and brown scallops throughout the entire thermal stress period compared to control (unstressed scallops), suggesting that CnHSP90 might play an important role in protecting organisms from further damage. This observation is consistent with earlier reports in Laternula elliptica exposed to thermal stress (Kim et al 2009) and in Argopecten irradians after heat shock (Yang et al 2014).…”

Section: Discussionsupporting

confidence: 94%

Effects of thermal stress on mortality and HSP90 expression levels in the noble scallops Chlamys nobilis with different total carotenoid content

Cheng

Liu

Zhang

et al. 2020

Cell Stress and Chaperones

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The noble scallop Chlamys nobilis is an economically important marine bivalve cultivated in the southern sea of China since the 1980s. Unfortunately, mass mortality of this scallop species often occurs in summer. The present study was conducted to investigate whether the expression of heat shock protein 90 (HSP90) and level of carotenoids could enhance high-temperature stress resistance in scallop. First, the HSP90 homolog of C. nobilis (designated CnHSP90) was identified and cloned. The complete cDNA sequence of CnHSP90 was 2631 bp, including a 2181-bp open reading frame (ORF) encoding a 726 amino acid polypeptide with five HSP90 family signatures, and sharing high homology with members of the HSP90 family. CnHSP90 was ubiquitously expressed in all examined tissues including the intestine, kidney, adductor, mantle, gill, and gonad, with the highest in the gonad. Golden and brown scallops, which contain significantly different total carotenoid content (TCC), were subjected to acute thermal challenge, and the LTE 50 (semi-lethal temperature at 36 h heat shock) and LTI 50 (semi-lethal time after heat shock) as well as the correlation between CnHSP90 gene expression and TCC were determined. The LTE 50 of golden scallop (32.14°C) was higher than that of brown scallops (31.19°C), with longer LTI 50 at all tested temperatures, indicating that golden scallops were more resistant to thermal stress than brown scallops. Similarly, the mRNA expression levels of CnHSP90 in gill of golden scallops were significantly higher (P < 0.05) than that of brown scallops at 6, 12, 24, and 36 h, with a strong positive correlation between CnHSP90 expression level and TCC. This suggests that both carotenoids and HSP90 levels could improve thermal resistance in the noble scallops.

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“…Subsequently, the induction of hsps has been widely used as a strong indicator to monitor intracellular thermal stress conditions. The preferential induction of hsps upon thermal stress is also widely reported in numerous mollusks; abalone Haliotis tuberculate hsp70 and hsp90 (Farcy et al, 2007), Antarctic clam Laternula elliptica hsp70 and hsp90 (Park et al, 2007;Kim et al, 2009), Antarctic intertidal limpet Nacella concinna hsp70 (Clark et al, 2008), eastern oyster Crassostrea virginica hsp60, hsp70, and hsp90 (Ivanina et al, 2009), European flat oyster Ostrea edulis hsp70 (Piano et al, 2004), limpet Lottia species hsp70 (Dong et al, 2008), intertidal snail Echinolittorina malaccana hsp gene families (Wang et al, 2014), Pacific oyster C. gigas hsp23, hsp70, and hsc70 (Clegg et al, 1998;Hamdoun et al, 2003;Meistertzheim et al, 2007;Zhu et al, 2016). These results strongly suggest that the induction of hsp gene families is an evolutionarily conserved mechanism upon temperature challenges.…”

Section: Discussionmentioning

confidence: 62%

Thermal stress induces a distinct transcriptome profile in the Pacific oyster Crassostrea gigas

Lim

Kim

Hwang

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica

Cited by 25 publications

References 39 publications

Expression pattern of heat shock proteins during acute thermal stress in the Antarctic sea urchin, Sterechinus neumayeri

Expression pattern of heat shock proteins during acute thermal stress in the Antarctic sea urchin, Sterechinus neumayeri

Effects of thermal stress on mortality and HSP90 expression levels in the noble scallops Chlamys nobilis with different total carotenoid content

Thermal stress induces a distinct transcriptome profile in the Pacific oyster Crassostrea gigas

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