Identification of Two Duplicated Loci Controlling a Disease‐like Rugose Leaf Phenotype in Soybean

Wang, Yaqi; Chen, Wei; Zhang, Ye; Liu, Meifeng; Kong, Jiejie; Yu, Zheping; Jaffer, Ali; Gai, Junyi; Zhao, Tuanjie

doi:10.2135/cropsci2015.09.0580

Cited by 4 publications

(6 citation statements)

References 37 publications

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“…Polymorphic markers that may be linked with the mutated genes were screened using the method of bulked-segregant analysis (BSA), as proposed by Michelmore et al [86]. Both wild-type and mutant groups contained 10 randomly selected F 2 individuals, and the protocol was according to Wang et al [19]. Within each group, the DNA from all individuals was pooled using an equal amount of DNA from each plant.…”

Section: Methodsmentioning

confidence: 99%

“…Mapmaker 3.0 software (Whitehead Institute for Biomedical Research, Cambridge, MA, USA) was used to identify the linkage between SSR markers and target genes [87]. A total of 307 recessive mutant plants from the F 2 and F 2:3 populations of W82× spl-1 and PI378692× spl-1 crosses were used for preliminary and fine mapping (Table 1), and the protocol was according to Wang et al [19].…”

Section: Methodsmentioning

confidence: 99%

“…Inheritance of dlm mutant phenotype and some leaf morphological traits were carried out, and it was reported the dlm allele inherited independently with that of P1 , y9 , f , lf2 and ti alleles controlling glabrous, chlorophyll-deficient leaf, stem trait, seven leaflet and trypsin inhibitors traits in soybean, respectively [17,18]. Wang et al [19] found a new LMM in soybean with rugose leaf phenotype controlled by two duplicated genes, rf1 , and rf2 , which were mapped on chromosome 18 and 08, respectively. Although few LMMs have been characterized in soybean, the genes underlying the mutant phenotype have not been cloned yet.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Golpour

Kong

Sharmin

et al. 2019

IJMS

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In plants, lesion mimic mutants (LMMs) reveal spontaneous disease-like lesions in the absence of pathogen that constitutes powerful genetic material to unravel genes underlying programmed cell death (PCD), particularly the hypersensitive response (HR). However, only a few LMMs are reported in soybean, and no related gene has been cloned until now. In the present study, we isolated a new LMM named spotted leaf-1 (spl-1) from NN1138-2 cultivar through ethyl methanesulfonate (EMS) treatment. The present study revealed that lesion formation might result from PCD and excessive reactive oxygen species (ROS) accumulation. The chlorophyll content was significantly reduced but antioxidant activities, viz., superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), as well as the malondialdehyde (MDA) contents, were detected higher in spl-1 than in the wild-type. According to segregation analysis of mutant phenotype in two genetic populations, viz., W82×spl-1 and PI378692×spl-1, the spotted leaf phenotype of spl-1 is controlled by a single recessive gene named lm1. The lm1 locus governing mutant phenotype of spl-1 was first identified in 3.15 Mb genomic region on chromosome 04 through MutMap analysis, which was further verified and fine mapped by simple sequence repeat (SSR) marker-based genetic mapping. Genetic linkage analysis narrowed the genomic region (lm1 locus) for mutant phenotype to a physical distance of ~76.23 kb. By searching against the Phytozome database, eight annotated candidate genes were found within the lm1 region. qRT-PCR expression analysis revealed that, among these eight genes, only Glyma.04g242300 showed highly significant expression levels in wild-type relative to the spl-1 mutant. However, sequencing data of the CDS region showed no nucleotide difference between spl-1 and its wild type within the coding regions of these genes but might be in the non-coding regions such as 5′ or 3′ UTR. Hence, the data of the present study are in favor of Glyma.04g242300 being the possible candidate genes regulating the mutant phenotype of spl-1. However, further validation is needed to prove this function of the gene as well as its role in PCD, which in turn would be helpful to understand the mechanism and pathways involved in HR disease resistance of soybean.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Golpour

Kong

Sharmin

et al. 2019

IJMS

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“…Bulked-segregant analysis (BSA) was used to determine SSR (simple sequence repeat) markers linked to NT302 locus as described by Michelmore et al (1991). 10 individuals with wild-type and mutant phenotype were randomly selected to form two contrasting groups, respectively (Wang et al, 2016a). Equal amount of DNA for 10 individuals of each group were pooled separately.…”

Section: Inheritance Analysis Genetic Mapping and Map-based Cloning mentioning

confidence: 99%

Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

Wang

Liu

et al. 2020

The Plant Journal

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Summary Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) are two important members of P450 enzymes metabolizing hydroperoxy fatty acid to produce jasmonates and aldehydes respectively, which function in response to diverse environmental and developmental stimuli. However, their exact roles in soybean have not been clarified. In present study, we identified a lesion‐mimic mutant in soybean named NT302, which exhibits etiolated phenotype together with chlorotic and spontaneous lesions on leaves at R3 podding stage. The underlying gene was identified as GmHPL encoding hydroperoxide lyase by map‐based cloning strategy. Sequence analysis demonstrated that a single nucleotide mutation created a premature termination codon (Gln20‐Ter), which resulted in a truncated GmHPL protein in NT302. GmHPL RNA was significantly reduced in NT302 mutant, while genes in AOS branch of the 13‐LOX pathway were up‐regulated in NT302. The mutant exhibited higher susceptibility to bacterial leaf pustule (BLP) disease, but increased resistance against common cutworm (CCW) pest. GmHPL was significantly induced in response to MeJA, wounding, and CCW in wild type soybean. Virus induced gene silencing (VIGS) of GhHPL genes gave rise to similar lesion‐mimic leaf phenotypes in upland cotton, coupled with upregulation of the expression of JA biosynthesis and JA‐induced genes. Our study provides evidence that competition exist between HPL and AOS branches in 13‐LOX pathway of the oxylipin metabolism in soybean, thereby plays essential roles in modulation of plant development and defense.

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“…For example, among the four homologous genes of the Arabidopsis terminal flower gene (TFL1) in soybean, only one has been found to control growth habit; the other copies may have additional functions because they have been reported to show different transcriptional patterns [30]. A disease-like rugose leaf phenotype in soybean was attributed to two recessive duplicated loci of rl1 and rl2 [31]. In our study, all genes located within the mapping regions of dsp1 and dsp2 loci have one or more duplicated copies in other chromosomes.…”

Section: Glymawm82 A2v1 Gene Models Descriptionmentioning

confidence: 99%

Genetic Analysis and Gene Mapping for a Short-Petiole Mutant in Soybean (Glycine max (L.) Merr.)

et al. 2019

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Short petiole is a valuable trait for the improvement of plant canopy of ideotypes with high yield. Here, we identified a soybean mutant line derived short petiole (dsp) with extremely short petiole in the field, which is obviously different from most short-petiole lines identified previously. Genetic analysis on 941 F2 individuals and subsequent segregation analysis of 184 F2:3 and 172 F3:4 families revealed that the dsp mutant was controlled by two recessive genes, named as dsp1 and dsp2. Map-based cloning showed that these two recessive genes were located on two nonhomologous regions of chromosome 07 and chromosome 11, of which the dsp1 locus was mapped at a physical interval of 550.5-Kb on chromosome 07 near to centromere with flanking markers as BARCSOYSSR_07_0787 and BARCSOYSSR_07_0808; whereas, the dsp2 locus was mapped to a 263.3-Kb region on chromosome 11 with BARCSOYSSR_11_0037 and BARCSOYSSR_11_0043 as flanking markers. A total of 36 and 33 gene models were located within the physical genomic interval of dsp1 and dsp2 loci, respectively. In conclusion, the present study identified markers linked with genomic regions responsible for short-petiole phenotype of soybean, which can be effectively used to develop ideal soybean cultivars through marker-assisted breeding.

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Identification of Two Duplicated Loci Controlling a Disease‐like Rugose Leaf Phenotype in Soybean

Cited by 4 publications

References 37 publications

Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)

Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

Genetic Analysis and Gene Mapping for a Short-Petiole Mutant in Soybean (Glycine max (L.) Merr.)

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