Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

Abstract

Summary Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) are two important members of P450 enzymes metabolizing hydroperoxy fatty acid to produce jasmonates and aldehydes respectively, which function in response to diverse environmental and developmental stimuli. However, their exact roles in soybean have not been clarified. In present study, we identified a lesion‐mimic mutant in soybean named NT302, which exhibits etiolated phenotype together with chlorotic and spontaneous lesions on leaves at R3 podding stage. The underlying gene was identified as GmHPL encoding hydroperoxide lyase by map‐based cloning strategy. Sequence analysis demonstrated that a single nucleotide mutation created a premature termination codon (Gln20‐Ter), which resulted in a truncated GmHPL protein in NT302. GmHPL RNA was significantly reduced in NT302 mutant, while genes in AOS branch of the 13‐LOX pathway were up‐regulated in NT302. The mutant exhibited higher susceptibility to bacterial leaf pustule (BLP) disease, but increased resistance against common cutworm (CCW) pest. GmHPL was significantly induced in response to MeJA, wounding, and CCW in wild type soybean. Virus induced gene silencing (VIGS) of GhHPL genes gave rise to similar lesion‐mimic leaf phenotypes in upland cotton, coupled with upregulation of the expression of JA biosynthesis and JA‐induced genes. Our study provides evidence that competition exist between HPL and AOS branches in 13‐LOX pathway of the oxylipin metabolism in soybean, thereby plays essential roles in modulation of plant development and defense.

CsHCT-Mediated Lignin Synthesis Pathway Involved in the Response of Tea Plants to Biotic and Abiotic Stresses

“…The exposure of plants to biological stresses such as pests and diseases often causes the accumulation of jasmonic acid and its derivatives. 47,48 Figure 2 indicates that when tea plants were treated with exogenous jasmonic acid methyl ester (MeJA), the expression of most of the genes in the three pathways was upregulated, but the upregulation of lignin pathway gene expression was greater than that of flavonoid pathway gene expression.…”

Molecular Breeding to Overcome Biotic Stresses in Soybean: Update

“…In another experiment, a BLP resistance gene/QTL was reported to be located near (21.5 cM) to the SSR marker Sat_108 on Chr 10 [ 241 , 242 ]. In a recent study, Wang et al [ 244 ] identified the role of GmHPL in Xag resistance in soybean.…”

Progress in soybean functional genomics over the past decade

“…Interestingly, genes encoding small peptides, such as GmSubPep , encoding a 12‐amino acid peptide (Pearce et al ., 2010), and GmPep914 , encoding an 8‐amino acid peptide (Yamaguchi et al ., 2011), also played important roles in defence against disease. It was recently found that hydroperoxide lyase modulated the defence response and conferred lesion‐mimic phenotype in soybean leaves (Wang et al ., 2020f).…”