Identification of tumor-associated antigens in human thyroid papillar carcinoma

Abstract

In this study two cDNA expressing libraries generated from thyroid papillar carcinomas were screened using SEREX approach. Thirty positive cDNA clones representing seventeen different genes were identified from both libraries. It is important to note, that three of them were isolated previously by other laboratories in SEREX screens of various types of human cancer. These include transcription factor NZF, a-catenin and ВАС RPU-a protein with unknown function. Moreover, we identified a whole panel of novel potential tumor-associated antigens, which would be further investigated. We are particularly interested in more detailed analysis of cathepsin H and transducer of ErbB2 (TOB2), which are differentially expressed in various types of human cancer. We will analyse the frequency of autoantibodies against identified antigens in sera of patients with various malignancies and healthy donors by heterologous screening. It is expected that among the clones isolated in this study, there might be novel cancer-associated markers.

Autoantibody Response to ZRF1 and KRR1 SEREX Antigens in Patients with Breast Tumors of Different Histological Types and Grades

“…Today more than 2000 TAAs have been identified by different approaches including SEREX, SEPRA, phage display, microarray, and other high throughput technologies [14, 23]. Previously, we have described 81 autoantigens identified from breast, colon, and thyroid cancers by SEREX approach [10, 12, 13, 15, 16]. Original modification of SEREX technique for the generation of medullary breast carcinoma cDNA expression library depleted from IgG genes has been developed [12].…”

Tumor-associated antigens and development of immunotherapeutics strategies

“…During the last few years, SEREX has been applied to a wide range of tumor types, including melanoma, renal cancer, astrocytoma, Hodgkin's di sease [15], oesophageal cancer [21], gastric cancer [22], colon cancer [23], lung cancer [24], breast cancer [25], prostate cancer [26], leukaemia [27] and thyroid cancer [28]. For the systematic docu mentation and archiving of sequence data and im munological characteristics of identified antigens, the electronic SEREX database, established by the LICR, is accessible to the public http://www.licr.org/SE-REX.html.…”