Identification of triterpenoid compounds of <i>Centella asiatica</i> by thin‐layer chromatography and mass spectrometry

Bonfill, Mercedes; Mangas, Susana; Cusidó, Rosa M.; Osuna, Lidia; Piñol, M. Teresa; Palazón, Javier

doi:10.1002/bmc.564

Cited by 71 publications

(50 citation statements)

References 3 publications

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“…The medicinal value of this plant was revealed in Charaka Chikitsa [4] and main active essential elements of C. asiatica (L.) are asiatic acid, madecasic acid, asiaticoside, madecassoside, brahmoside, brahmic acid, brahminoside, thankiniside, isothankunisode, centelloside, madasiatic acid, alkaloids, flavanoids, etc. [5][6][7][8][9][10] which are known to take care of skin problems, to heal wounds, for stimulating the nerves and brain cells, antileprotic, antifilarial, antibacterial, adaptogenic, antifeedant, anti-stress, anti-ulcer, antioxidative stress, anti-radiation properties, anti-heavy metal poisoning, antiviral properties, anticancer etc. [11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Roy¹,

Kundu²,

Saxena³

et al. 2017

J Plant Biochem Physiol

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Due to the presence of several bioactive compounds, Centella asiatica (L.) is used in traditional medicine for the treatment of various ailments. This usage of plant and its products created a huge demand which required an alternative method of its commercial production so that the loss of natural plant and its genetic pool can be prevented. A different investigation using biotechnological methods provided a wealth of information to enhance the biomass as well as different bioactive compounds. In the present study, we focused and reported the effect of different nitrogen sources on shoot proliferation of Centella asiatica. Effect of malt extract as plant elicitor on asiaticoside production as well as fatty acid methyl ester (FAME) profile was also worked out. Four different nitrogen sources i.e., NH 4 NO 3 (1.65 g/l), KNO 3 (0.8 g/l), NaNO 3 (1.65 g/l), Ca(NO 3 ) 2 (0.825 g/l) on five different accessions of Centella asiatica were tested and accession number 347492 (14.66 ± 2.4) provided maximum shoot proliferation with ammonium nitrate. RP-HPLC analysis of accession number 347492 revealed that malt extract as plant extract enhanced 6 times asiaticoside in comparison to standard conditions. GC-MS analysis of five different accessions of Centella asiatica concluded that this plant is rich in the content of Pentadecanoic acid, 9, 12 Octadecadienoic acid (Linoleic acid) and 9, 12, 15 Octadecatrienoic acid (Linolenic acid). Most potential fatty acid methyl esters containing plant accession number is 281374.

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Section: Introductionmentioning

confidence: 99%

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Roy¹,

Kundu²,

Saxena³

et al. 2017

J Plant Biochem Physiol

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“…AA has also been shown to display neuroprotective properties both in vitro and in vivo. 2) In cellular systems, AA is neuroprotective against rotenone and H 2 O 2 -induced injury in SH-SY5Y cells 3) and offers protection against β-amyloid-induced cell death in the neuroblastoma B103 cell line. 4,5) It also reduced H 2 O 2 -related cell death and decreased intracellular free radical concentrations.…”

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confidence: 99%

Neuroprotective Effect of Asiatic Acid in Rat Model of Focal Embolic Stroke

Lee

Bae

Weinstock

et al. 2014

Biological & Pharmaceutical Bulletin

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Asiatic acid (AA) is a pleiotropic neuroprotective agent that has been shown to attenuate infarct volume in mouse and rat models of focal ischemia and has a long clinically relevant therapeutic time-window. Because in a future trial AA would be administered with tissue-plasminogen activator (t-PA), the only approved acute stroke therapy, we sought to determine the effect of AA when co-administered with t-PA in a rat focal embolic stroke model. Male rats were treated with AA (75 mg/kg) alone, low-dose t-PA (2.5 mg/kg) alone, or a combination of AA and low-dose t-PA at 3 h after inducing embolic stroke. AA significantly reduced infarct volume whereas low-dose t-PA alone did not reduce infarct volume compared with vehicle. Significantly, combination treatment further enhanced reduction of infarct volume versus AA alone. Treatment with AA reduced cytochrome c (CytoC) and apoptosis-inducing factor (AIF) release from brain mitochondria after ischemia. AA was also neuroprotective against L-glutamate-induced toxicity in primary cortical neurons. In summary, combination treatment with AA and low-dose t-PA at 3 h after embolic stroke reduces infarct volume, improves neurological outcome, and provides neuroprotection. The neuroprotective effects of AA were partially associated with reduction of AIF and CytoC release.Key words asiatic acid; tissue-plasminogen activator (t-PA); neuroprotection; rat embolic stroke model; stroke Stroke is a leading cause of mortality and the largest single cause of adult disability worldwide.1) Tissue-plasminogen activator (t-PA) is the only approved pharmacological therapy. However, thrombolysis with t-PA is limited by its narrow time window and the risk of hemorrhagic complications. There is a desperate need for additional safe and effective agents. Asiatic acid (AA) is a triterpene isolated from Centella asiatica which has been widely used as an antioxidant and anti-inflammatory herb in Ayurvedic medicine. AA has also been shown to display neuroprotective properties both in vitro and in vivo.2) In cellular systems, AA is neuroprotective against rotenone and H 2 O 2 -induced injury in SH-SY5Y cells 3) and offers protection against β-amyloid-induced cell death in the neuroblastoma B103 cell line. 4,5) It also reduced H 2 O 2 -related cell death and decreased intracellular free radical concentrations.4) Furthermore, AA derivatives rescue primary rat cortical cells from glutamate-induced toxicity.6) Also, AA was recently reported to alleviate hemodynamic and metabolic alterations via restoring endothelial nitric oxide synthase (eNOS)/inducible nitric oxide synthase (iNOS) expression, oxidative stress, and inflammation in diet-induced metabolic syndrome rats and to regulates the carbohydrate metabolism by modulating the key regulatory enzymes in diabetic rats. 7,8) AA inhibits adipogenic differentiation of bone marrow stromal cells and attenuates ethanol-induced hepatic injury via suppression of oxidative stress and Kupffer cell activation. 9,10)

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“…The putative anxiolytic activity of asiaticoside was examined in male mice by using a number of experimental paradigms of anxiety, with diazepam as a positive anxiolytic control. The asiaticoside present in Centella asiatica extract was identified and quantified by HPLC and TLC analysis (Rafamantanana et al, 2009;Zhang et al, 2008;Bonfill et al, 2006). The structure of the asiaticoside was established by Polonsky et al (1959) and is similar to the one as shown on the Figure 1.…”

Section: Introductionmentioning

confidence: 88%

Densitometric HPTLC quantification of asiaticoside isolated from Centella asiatica (L.) Urb (Apiaceae) of Benin

Ganse¹,

Gbaguidi²,

Agbani³

et al. 2011

International Journal of Biological and Chemical Sciences

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Asiaticoside isolated from Centella asiatica has been found through in vitro test to serve as an active agent of healing on wounds. To quantify this compound in Centella asiatica cultivated in Benin, a new, simple and rapid High-Performance Thin-Layer Chromatography (HPTLC) method was developed and validated for its selectivity, its recovery, and its repeatability. Compounds have been separated on silica gel 60F 254 plates with ethyl acetate/methanol/water (100/25/10, v/v/v), as mobile phase. The detection was done by densitometry scanning at λ = 600 nm and the calibration plots showed that the graphical response was linear and dependent on the quantity in the range of 2.50-12.50 µg, with good values of R² = 0.998. This method is repeatable and precise with relative standard deviations between1.42 and 5.92% for the intra-day tests and between 1.27 and 6.57% for the inter-day tests for the quantities belonging to an interval of 2.50 and 12.50 µg. The limits of detection and quantification were 0.317 and 1.05 µg respectively. The quantity of asiaticoside found was 0.24% in the alcoholic extract.

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Identification of triterpenoid compounds of Centella asiatica by thin‐layer chromatography and mass spectrometry

Cited by 71 publications

References 3 publications

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Neuroprotective Effect of Asiatic Acid in Rat Model of Focal Embolic Stroke

Densitometric HPTLC quantification of asiaticoside isolated from Centella asiatica (L.) Urb (Apiaceae) of Benin

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