Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

Abstract

Due to the presence of several bioactive compounds, Centella asiatica (L.) is used in traditional medicine for the treatment of various ailments. This usage of plant and its products created a huge demand which required an alternative method of its commercial production so that the loss of natural plant and its genetic pool can be prevented. A different investigation using biotechnological methods provided a wealth of information to enhance the biomass as well as different bioactive compounds. In the present study, we focused and reported the effect of different nitrogen sources on shoot proliferation of Centella asiatica. Effect of malt extract as plant elicitor on asiaticoside production as well as fatty acid methyl ester (FAME) profile was also worked out. Four different nitrogen sources i.e., NH 4 NO 3 (1.65 g/l), KNO 3 (0.8 g/l), NaNO 3 (1.65 g/l), Ca(NO 3 ) 2 (0.825 g/l) on five different accessions of Centella asiatica were tested and accession number 347492 (14.66 ± 2.4) provided maximum shoot proliferation with ammonium nitrate. RP-HPLC analysis of accession number 347492 revealed that malt extract as plant extract enhanced 6 times asiaticoside in comparison to standard conditions. GC-MS analysis of five different accessions of Centella asiatica concluded that this plant is rich in the content of Pentadecanoic acid, 9, 12 Octadecadienoic acid (Linoleic acid) and 9, 12, 15 Octadecatrienoic acid (Linolenic acid). Most potential fatty acid methyl esters containing plant accession number is 281374.

The protective effect of Indian Catechu methanolic extract against aluminum chloride-induced neurotoxicity, A rodent model of Alzheimer's disease

“…Hence ICME can play a role in many diseases in which oxidative stress is a major factor in its pathogenesis as the case in AD. This assumption is supported by other studies that showed the potential of some plants in AD based on their antioxidant and free radicle scavenging activities as Withanamides, Bacopa monnieri and Centella asiatica plants ( Limpeanchob et al., 2008 ; Jayaprakasam et al., 2010 ; Roy et al., 2017 ).…”

Fatty Acid Methyl Ester Profile Analysis of In-Vitro Grown Accessions of Plumbago zeylanica

“…Thus it can be concluded that this plant is a good source of fatty acids and can be used in cholesterol-lowering diets. Similar study has been conducted by Roy et al [15]…”

Role of medicinal plants against Alzheimer’s disease

“…23 Asiatic acid and asiaticoside showed reduce hydrogen peroxide-induced cell death, decline concentration of free radicals, and β-amyloid cell death inhibition in vitro which suggested possible role in Alzheimer's disease treatment and β-amyloid toxicity prevention. [24][25][26] Extracts of Centella asiatica reversed the β-amyloid pathology in mice brains and modulated oxidative stress response components. 27 It is an important plants for nerve and brain cells and considers being capable of enhancing intellect, memory and longevity.…”