Identification of Trichomonas vaginalis Cysteine Proteases That Induce Apoptosis in Human Vaginal Epithelial Cells

Sommer, Ulf; Costello, Catherine E.; Hayes, Gary R.; Beach, David; Gilbert, R.O.; Lucas, John J.; Singh, Bibhuti N.

doi:10.1074/jbc.m501752200

Cited by 108 publications

(123 citation statements)

References 49 publications

(70 reference statements)

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“…Recently, it has been demonstrated that T. foetus induces apoptotic cell death in bovine vaginal epithelial cells [18] and in cultured bovine uterine epithelial cells [19]. In addition, Sommer et al [21] showed that T. vaginalis cystein proteases can induce apoptosis in human epithelial cells. Programmed cell death (PCD) is a genetically regulated physiological process of cell demise that is central to the development and homeostasis of multicellular organisms [20].…”

Section: Discussionmentioning

confidence: 99%

Tritrichomonas foetusdamages bovine oocytes in vitro

Benchimol¹,

Fontes²,

Dias³

2007

Vet. Res.

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-Tritrichomonas foetus is an extracellular parasite of the urogenital tract in cattle. It causes infertility and abortion, but there is no documented information on the susceptibility of bovine oocytes to the parasite, except by one article that claimed no effects of T. foetus on oocytes or embryos. The aim of the present study was to study the effects provoked by T. foetus when in interaction with bovine oocytes. Oocytes were obtained from cow ovaries and divided into two groups: (1) one group contained cumulus cells, whereas (2) a second group was denuded from these cells. Light microscopy, video microscopy and scanning electron microscopy (SEM) revealed that exposure of oocytes to T. foetus caused rapid adhesion of the trichomonads to cumulus cells and to the zona pellucida (ZP). Motile parasites were observed for 12 h. The ZP was completely damaged, and the parasites were able to infiltrate beneath the ZP and reached the oocytes directly when the oocytes were denuded of the cumulus cells. Both the oocytes and the cumulus cells exhibited morphological characteristics compatible with apoptosis after interaction with T. foetus, such as chromatin condensation, the presence of several cytoplasmic vacuoles, with intact cellular membranes and organelles. The results from this study demonstrate that when a large number of T. foetus interacts with oocytes in vitro damage and apoptosis are provoked in the cow's reproductive cells. The behavior of this parasite as one of the causes of cattle infertility is discussed.Tritrichomonas foetus / oocytes / cow / ultrastructure / infertility

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Section: Discussionmentioning

confidence: 99%

Tritrichomonas foetusdamages bovine oocytes in vitro

Benchimol¹,

Fontes²,

Dias³

2007

Vet. Res.

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“…Antibodies against T. vaginalis were detected in circulation and vaginal mucosa of infected women [8,22,23]. T. vaginalis cysteine proteases including CP30 may induce apoptosis of vaginal epithelial cells and multiple mucosal immune cell types [8,24]. These immunological changes may be a cause of infertility.…”

Section: T Vaginalis In Infertile Turkish Women Discussionmentioning

confidence: 99%

Investigation of the presence of Trichomonas vaginalis in infertile Turkish women

et al. 2015

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Background: Trichomoniasis is a sexually transmitted vaginal infection caused by a protozoan called Trichomonas vaginalis. Its prevalence ranges between 3−40% among randomly tested women. Objectives: To investigate the presence of T. vaginalis in infertile women presenting to a Turkish gynecology outpatient clinic using parasitological methods. Methods:The study population comprised 51 patient participants who attended the gynecology clinic of Mustafa Kemal University Hospital between March and August 2013. The women were divided into those presenting with infertility (n = 22) and a patient control group (n = 29). Women in the control group had complaints other than infertility. Microscopic examination, Giemsa staining, and cysteine-peptone-liver-maltose culture were performed on samples taken from posterior fornix tested for T. vaginalis. Results: T. vaginalis was observed in 18% (n = 4) of infertile patients and in none of the control group (P = 0.03). Conclusions: Asymptomatic infertile women should be examined for T. vaginalis infection, which may play a role as a cause or contributing factor in infertility.

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“…Najważniejsze mechanizmy patogenezy stwierdzone u tego gatunku to ekspresja adhezyn AP65, AP51, AP33, AP23, za pomocą których pasożyt przywiera do komórek nabłonka gospodarza, po czym uszkadza je uwalnianymi przez siebie enzymami (50,56). Potwierdzono wytwarzanie przez T. vaginalis enzymów hydrolitycznych: proteinazy cysteinowej (CP 65), fosfatazy kwaśnej, proteazy GP63 oraz β-N-acetyloglukozaminidazy (67,87,96). Występowanie znacznej aktywności proteolitycznej stwierdzono także w przypadku T. tenax.…”

Section: Chorobotwórczość I Czynniki Zjadliwościunclassified

Systematics, biology and invasiology of trichomonads (Trichomonadida) - the current state of knowledge

Szczepaniak¹,

Tomczuk²,

Łojszczyk-Szczepaniak³

et al. 2017

Medycyna Weterynaryjna

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Trichomonads are anaerobic, flagellated protists belonging to the large phylum Parabasalia. In most cases, they exist as endosymbionts – harmless comensals of invertebrates and vertebrates – but several species are considered to be imported intestinal or urogenital parasites of humans and other animals. This paper presents current information on the taxonomy, morphology and biology of trichomonads. The characterization of invasiology followed by the description of virulence factors and pathogenic mechanisms of trichomonads is presented. The present paper also reviews diagnostic methods, treatment and prevention of trichomoniasis.

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Identification of Trichomonas vaginalis Cysteine Proteases That Induce Apoptosis in Human Vaginal Epithelial Cells

Cited by 108 publications

References 49 publications

Tritrichomonas foetusdamages bovine oocytes in vitro

Tritrichomonas foetusdamages bovine oocytes in vitro

Investigation of the presence of Trichomonas vaginalis in infertile Turkish women

Systematics, biology and invasiology of trichomonads (Trichomonadida) - the current state of knowledge

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