Identification of tomoregulin-1 as a novel addicsin-associated factor

Arano, Taku; Fujisaki, Shingo; Ikemoto, Mitsushi

doi:10.1016/j.neuint.2014.03.011

Cited by 11 publications

(11 citation statements)

References 44 publications

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“…As RANKL can form homo-or heteromultimers [25], our finding supported that Arl6ip5 was inclined to bind with the proteins that have the property to form oligomerization [17]. Evidences from the homologue of Arl6ip5 demonstrated that the COOH-terminal domain of Arl6ip5 contained the signal for its ER localization and was important for the binding with proteins such as TR1 [26,27]. However, it was the NH 2 -terminal but not the COOH-terminal responsible for the interaction between Arl6ip5 and RANKL, as revealed by immunoprecipitation analysis with types of truncates.…”

Section: Discussionsupporting

confidence: 71%

Overexpression of Arl6ip5 in osteoblast regulates RANKL subcellualr localization

Wang

Peng

et al. 2015

Biochemical and Biophysical Research Communications

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Section: Discussionsupporting

confidence: 71%

Overexpression of Arl6ip5 in osteoblast regulates RANKL subcellualr localization

Wang

Peng

et al. 2015

Biochemical and Biophysical Research Communications

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“…In the case of independent expression, TMEFF1 was preferentially expressed in the cell membrane. However, after the binding of TMEFF1 to addicsin, which is a new protein in the amygdala of mice, the distribution of TMEFF1 in the cell membrane was inhibited so that TMEFF1 mainly redistributed to the endoplasmic reticulum and suppressed the migration of cells 34 . This indicates that TMEFF1 participates in the migration process of cells.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of TMEFF1 in Endometrial Carcinoma and the Mechanism Underlying its Promotion of Malignant Behavior in Cancer Cells

et al. 2021

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Background: Although tomoregulin-1 (TMEFF1) is involved in embryonic development and central nervous system regulation and is a cancer suppressor gene in brain cancers, its role in endometrial carcinoma remains unclear. Methods: The expression and prognostic value of TMEFF1 were analyzed by bioinformatics methods and immunohistochemistry. An endometrial carcinoma cell line with low expression of TMEFF1 was constructed. Scratch and Transwell assays were used to determine the effect of TMEFF1 on cell invasion and migration. Changes in key proteins in the MAPK and PI3K/AKT signaling pathways and in epithelial-mesenchymal transition (EMT)-related proteins were analyzed using western blot. Chromatin immunoprecipitation assay (ChIP) was performed to identify whether the TMEFF1 promoter region binds to the transcription factor p53. Results: TMEFF1 was significantly upregulated in endometrial carcinoma, was closely associated with FIGO stage ( P =0.021) and lymph node metastasis ( P =0.029), and was an independent risk factor for prognosis ( P =0.044). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that TMEFF1 and its related genes are involved in the cell cycle, regulation of mitosis, epigenetics, neural development, cell biological signal transduction and some key signal pathways. We also identified kinases, microRNAs and a transcription factor network related to TMEFF1 and the effect of TMEFF1 mutation on prognosis. In vitro knockdown of TMEFF1 significantly inhibited cell invasion and migration. Knockdown of TMEFF1 inhibited Epithelial-mesenchymal transition (EMT) and activation of the MAPK and PI3K/AKT pathways. However, the transcription factor p53 was not found to regulate the TMEFF1 gene. Conclusion: TMEFF1 plays an important role in endometrial carcinoma and may thus be a potential anticancer therapeutic target for endometrial carcinoma.

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“…In addition, WB analysis was performed as previously described. 19) Silver staining analysis was conducted according to the attached manufacture's protocol.…”

Section: Methodsmentioning

confidence: 99%

3,4-Dihydroxybenzalacetone Inhibits the Propagation of Hydrogen Peroxide-Induced Oxidative Effect <i>via</i> Secretory Components from SH-SY5Y Cells

Ikemoto

Aihara

Ishii

et al. 2023

Biological & Pharmaceutical Bulletin

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The polyphenol derivative 3,4-dihydroxybenzalacetone (DBL) is the primary antioxidative component of the medicinal folk mushroom Chaga (Inonotus obliquus (persoon) Pilat). In this study, we investigated whether the antioxidative effect of DBL could propagate to recipient cells via secreted components, including extracellular vesicles (EVs), after pre-exposing SH-SY5Y human neuroblastoma cells to DBL. First, we prepared EV-enriched fractions via sucrose density gradient ultracentrifugation using conditioned medium from SH-SY5Y cells exposed to 100 µM hydrogen peroxide (H 2 O 2 ) for 24 h, with and without 1 h of 5 µM DBL pre-treatment. CD63 immuno-dot blot analysis demonstrated that fractions with density of 1.06-1.09 g/cm 3 had CD63-like immuno-reactivities. Furthermore, the 2,2-diphenyl-1-picrylhydrazyl assay revealed that the radical scavenging activity of fraction 11 (density of 1.06 g/cm 3 ), prepared after 24-h H 2 O 2 treatment, was significantly increased compared to that in the control group (no H 2 O 2 treatment). Notably, 1 h of 5 µM DBL pre-treatment or 5 min of heat treatment (100 °C) diminished this effect, although concentrating the fraction by 100 kDa ultrafiltration enhanced it. Overall, the effect was not specific to the recipient cell types. In addition, the uptake of fluorescent Paul Karl Horan-labeled EVs in concentrated fraction 11 was detected in all treatment groups, particularly in the H 2 O 2 -treated group. The results suggest that cell-to-cell communication via bioactive substances, such as EVs, in conditioned SH-SY5Y cell medium, propagates the H 2 O 2 -induced radical scavenging effect, whereas pre-conditioning with DBL inhibits it.

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Identification of tomoregulin-1 as a novel addicsin-associated factor

Cited by 11 publications

References 44 publications

Overexpression of Arl6ip5 in osteoblast regulates RANKL subcellualr localization

Overexpression of Arl6ip5 in osteoblast regulates RANKL subcellualr localization

Overexpression of TMEFF1 in Endometrial Carcinoma and the Mechanism Underlying its Promotion of Malignant Behavior in Cancer Cells

3,4-Dihydroxybenzalacetone Inhibits the Propagation of Hydrogen Peroxide-Induced Oxidative Effect <i>via</i> Secretory Components from SH-SY5Y Cells

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