Identification of thyroid hormone receptors α and β genes and their expression profiles during metamorphosis in Rana chensinensis*

Abstract

To explore the role of thyroid hormones in organ remodeling during the metamorphosis of amphibians, the cDNAs of two thyroid hormone receptors (TRα and TRβ) in Rana chensinensis were identified. The 1257 bp of rcTRα cDNA encoding 418 amino acids and the 1122 bp of rcTRβ cDNA encoding 373 amino acids were cloned. Their polypeptide sequences contain two highly conserved cysteine-rich zinc fingers in the DNA-binding domain, while rcTRβ is 42 amino acids shorter in its A/B domain when compared with rcTRα. Fifty-five amino acids differed between the TRα and TRβ domains. The rcTRα and rcTRβ mRNA levels in the liver, brain, skin, and tail of tadpoles were analyzed via qRT-PCR during metamorphosis. The results showed that the changes of rcTRα mRNA levels were moderate while those of rcTRβ were dramatic in all 4 tissues during metamorphosis. In addition, the levels of rcTRβ mRNA were relatively low at premetamorphosis and reached a peak around the climax. Tissue-specific expression of rcTRα and rcTRβ suggests that thyroid hormones play various roles in different organs or tissues at different developmental stages of metamorphosis.

Tadpole serum activity (Rana catesbeiana) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in albino rats' epithelial cells induced by neoplasia

“…Increasing the number of CTL occurs due to the influence of tadpole serum ( R. catesbeiana ) containing the hormone thyroxine. Dendritic cells can help the thyroid hormone as a cancer vaccine to stimulate CTL as immunotherapy [ 7 ]. Other studies using L-thyroxine (T 4 ) and 3,3,5-triido-L-thyronine (T 3 ) could stimulate the proliferation of T lymphocytes [ 23 ].…”

“…Thyroxine hormone leads an important role in different organs and tissues [ 7 ]. Thyroxine hormone increases gene expression associated with proliferation, cell differentiation, and development of body shape in tadpoles [ 8 , 9 ].…”

Mitochondrial gene expression in different organs of Hoplobatrachus rugulosus from China and Thailand under low-temperature stress

“…All data were analyzed using Statistical Program for Social Sciences 21.0 software (SPSS, Inc., Chicago, IL, USA). Gene transcript levels in frog tissues from the control and low-temperature groups were compared using the Student’s t -TEST function, and P < 0.05 was considered statistically significant [ 67 ]. Data are presented graphically using Origin 8.0 software (Origin Lab).…”