Identification of the Surfactant Protein A Receptor 210 as the Unconventional Myosin 18A

Yang, Ching; Szeliga, Jacek; Jordan, Jeremy; Faske, Shawn; Sever-Chroneos, Zvjezdana; Dorsett, Bre; Christian, Robert E.; Settlage, Robert E.; Shabanowitz, Jeffrey; Hunt, Donald F.; Whitsett, Jeffrey A.; Chroneos, Zissis C.

doi:10.1074/jbc.m505229200

Cited by 54 publications

(122 citation statements)

References 84 publications

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“…33 The 787 bp MARCO DNA probe was generated from a pcDNA plasmid containing the 1.9kb cDNA of mouse MARCO. 34 The mouse MARCO cDNA was excised from the plasmid using BamHI and NotI.…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…34 The mouse MARCO cDNA was excised from the plasmid using BamHI and NotI. The excised cDNA was gel-purified as previously described 33 and further digested with Xmal, which generated two fragments of 787 and 1100 bp containing the 5′ and 3′ ends of mouse MARCO gene, respectively. The 787 fragment was purified using a Qiagen gel extraction kit (Qiagen, Valencia, CA), radio-actively labeled with 32 P, and used on Northern blots as described previously.…”

Section: Northern Blot Analysismentioning

confidence: 99%

“…The 787 fragment was purified using a Qiagen gel extraction kit (Qiagen, Valencia, CA), radio-actively labeled with 32 P, and used on Northern blots as described previously. 33 …”

Section: Northern Blot Analysismentioning

confidence: 99%

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Granulocyte–macrophage colony stimulating factor-mediated innate responses in tuberculosis

et al. 2008

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“…33 The 787 bp MARCO DNA probe was generated from a pcDNA plasmid containing the 1.9kb cDNA of mouse MARCO. 34 The mouse MARCO cDNA was excised from the plasmid using BamHI and NotI.…”

Section: Northern Blot Analysismentioning

confidence: 99%

Section: Northern Blot Analysismentioning

confidence: 99%

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Granulocyte–macrophage colony stimulating factor-mediated innate responses in tuberculosis

et al. 2008

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“…These data are consistent with previous studies in mice that showed that myosin 18Aa (p230) and b (p190) had different subcellular localizations, the former colocalizing with the endoplasmic reticulum and Golgi structures. 16 Myosin 18A was reported as a receptor for the surfactant protein A (SP-A), 17 a collectin notably present in human lung. 18 Thus, we performed immunoprecipitation with DY12 mAb using fresh human lung extracts.…”

Section: Resultsmentioning

confidence: 99%

“…Because (i) NK cells play a critical and first-line role in the antiviral immune defense, 20 (ii) human NK cells express high levels of CD245, (iii) CD245 cell surface expression is increased in the presence of IL-2 in NK cells and (iv) CD245 corresponds to myosin 18A that has been shown to be a receptor for SP-A, 17 a protein involved in viral clearance in the human lung, 21 we asked whether engagement of CD245 on the NK cell surface was able to regulate their IL-2-activated killer activity against virally infected cells. As expected, freshly isolated PB-NK lymphocytes failed to kill an EBV-infected B cell line and engagement of CD245 with DY12 mAb was unable to overcome the resistance to NK cell cytotoxicity of these highly MHC class I molecules expressing target cells (Fig.…”

Section: Cd245 Engagement By Dy12 Mab or By Its Physiological Ligandmentioning

confidence: 99%

Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes

et al. 2016

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CD245 is a human surface antigen expressed on peripheral blood lymphocytes, initially delineated by two monoclonal antibodies DY12 and DY35. Until now, CD245 molecular and functional characteristics remained largely unknown. We combined immunological and proteomic approaches and identified CD245 as the unconventional myosin 18A, a highly conserved motor enzyme reported as a receptor for the surfactant protein A (SP-A), that plays a critical role in cytoskeleton organization and Golgi budding. We report that the recruitment of CD245 strongly enhanced NK cell cytotoxicity. Further, we show that the enhancement of the NK lymphocytes killing ability toward CD137-ligand expressing target cells could result from the induction of CD137 expression following CD245 engagement. The SP-A receptor could therefore represent a novel and promising target in cancer immunotherapy.

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Myosin motors on the pathway of viral infections

2022

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Molecular motors are microscopic machines that use energy from adenosine triphosphate (ATP) hydrolysis to generate movement. While kinesins and dynein are molecular motors associated with microtubule tracks, myosins bind to and move on actin filaments. Mammalian cells express several myosin motors. They power cellular processes such as endo‐ and exocytosis, intracellular trafficking, transcription, migration, and cytokinesis. As viruses navigate through cells, they may take advantage or be hindered by host components and machinery, including the cytoskeleton. This review delves into myosins' cell roles and compares them to their reported functions in viral infections. In most cases, the previously described myosin functions align with their reported role in viral infections, although not in all cases. This opens the possibility that knowledge obtained from studying myosins in viral infections might shed light on new physiological roles for myosins in cells. However, given the high number of myosins expressed and the variety of viruses investigated in the different studies, it is challenging to infer whether the interactions found are specific to a single virus or can be applied to other viruses with the same characteristics. We conclude that the participation of myosins in viral cycles is still a largely unexplored area, especially concerning unconventional myosins.

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Identification of the Surfactant Protein A Receptor 210 as the Unconventional Myosin 18A

Cited by 54 publications

References 84 publications

Granulocyte–macrophage colony stimulating factor-mediated innate responses in tuberculosis

Granulocyte–macrophage colony stimulating factor-mediated innate responses in tuberculosis

Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes

Myosin motors on the pathway of viral infections

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