Identification of the species origin of highly processed meat products by mitochondrial DNA sequences.

Unseld, Michael; Beyermann, Birgit; Brandt, Petra; Hiesel, Rudolf

doi:10.1101/gr.4.4.241

Cited by 168 publications

(110 citation statements)

References 7 publications

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“…It is well known that in the case of severely degraded substrates the length of the fragment to be amplified should not exceed 200 bp (Unseld, Beyermann, Brandt, & Hiesel, 1995).…”

Section: Resultsmentioning

confidence: 99%

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Neve

Civera

Mucci³

et al. 2008

Meat Science

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The aim of the present study is to develop an assay for the specific identification of meat from Capreolus capreolus, Cervus elaphus, Capra ibex, Rupicapra rupicapra, targeting sequences of the cytochrome b (cyt b) gene of mitochondrial DNA. The assay is also intended to enable differentiation between meat from these wild species as well as Ovis aries, Capra hircus, Bubalus bubalis, Bos taurus and Sus scrofa domestic species.The primers used in the preliminary PCR were designed in well conserved regions upstream and downstream of the diagnosis sites. They successfully amplified a conserved 232 bp region from the cyt b gene of all the species taken into consideration. The sites of diagnosis have been interrogated using a minisequencing reaction and capillary electrophoresis. All the results of the multiplex PER (primer extension reaction) test were confirmed by fragment sequencing. The assay offers the possibility of discriminating nine species at the same time.

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“…It is well known that in the case of severely degraded substrates the length of the fragment to be amplified should not exceed 200 bp (Unseld, Beyermann, Brandt, & Hiesel, 1995).…”

Section: Resultsmentioning

confidence: 99%

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Neve

Civera

Mucci³

et al. 2008

Meat Science

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show abstract

“…Quinteiro et al [10] established a maximum fragment size in canned products of 176 bp to ensure the ampliWcation. Other authors, under certain conditions, ampliWed fragments higher than 200 bp from canned products [28][29][30][31].…”

Section: Dna Extractionmentioning

confidence: 99%

Development of a genetic method for the identification of salmon, trout, and bream in seafood products by means of PCR–RFLP and FINS methodologies

Espiñeira

Vieites

Santaclara

2009

Eur Food Res Technol

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In the present study, two alternative methods for identifying 13 salmon, trout and bream species were developed. Both of them are based on polymerase chain reaction (PCR) ampliWcation of a cytochrome b gene fragment. Subsequently, diVerent techniques were assayed to assign the PCR amplicons previously obtained to particular species. The Wrst one is based on the restriction fragment length polymorphism (RFLP) and includes three endonucleases for generating species-speciWc restriction proWles, while the second one is based on the phylogenetic analysis of DNA sequences. The main novelty of this work lies in the applicability of the developed methods to all kinds of processed products, including those undergoing intensive processes of transformation, as for instance canned foods. Finally, the methods were applied to 25 commercial samples including some that had been subjected to intensive thermal treatment, allowing the detection of those incorrectly labeled (16%). Therefore, these methods are useful to check the fulWllment of labeling regulations for seafood products, verify the correct traceability in commercial trade, and for Wsheries control.

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“…Both methods are characterised by very high sensitivity. The analysis of the results received with the help of the microscopic method and PCR has shown that molecular biology methods can be used as supplementary tools, according to the suggestions of different researchers (25,34,42). It results from the fact that the microscopic method gives credible results also in highly processed animal feeds, but with retained morphological structures, even when the genome material is highly degraded.…”

Section: Discussionmentioning

confidence: 99%

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

Weiner

Golebiowska

Paprocka

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and flotate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementary tools for detection and identification of bovine MBM.

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Identification of the species origin of highly processed meat products by mitochondrial DNA sequences.

Cited by 168 publications

References 7 publications

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Development of a genetic method for the identification of salmon, trout, and bream in seafood products by means of PCR–RFLP and FINS methodologies

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

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