Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Neve, Fabio La; Civera, Tiziana; Mucci, Nadia; Bottero, Maria Teresa

doi:10.1016/j.meatsci.2007.11.027

Cited by 43 publications

(27 citation statements)

References 20 publications

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“…In addition, Chikuni et al (1994); Matsunaga et al (1998);La Neve et al (2008) used cyt b gene sequence to identify of meats and meat products of red deer, roe deer, song thrush, pyrenean ibex, chamois, quail and sparrow.…”

Section: Pcr-sequencingmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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| Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.

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Section: Pcr-sequencingmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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show abstract

“…Multiplex primer extension reaction has been successfully applied in discriminating many animal and bacterial species in different food items [28][29][30][31]. However, some problems could arise when mixed matrices are considered.…”

Section: Resultsmentioning

confidence: 99%

Development of a real-time PCR assay for the detection of cow and donkey milk

Dalmasso

Sacchi

Bottero

2012

Eur Food Res Technol

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A real-time PCR allelic discrimination TaqMan assay based on the analysis of a single nucleotide polymorphism enabling the differentiation of cow (Bos taurus) and donkey (Equus asinus) milk was developed. Specific primers and probes were designed on the mitochondrial cytochrome c oxidase subunit I gene. The primers were designed upstream and downstream the chosen diagnosis site in a conserved region. Two probes were designed to specifically hybridise to B. taurus and E. asinus sequences. The test allowed the discrimination of bovine and donkey DNA in all blood and pure milk samples giving an unambiguous result plot of rapid and easy interpretation. The detection threshold was 2 % of cow milk in donkey milk. The applicability of the method to matrices containing degraded DNA was demonstrated by analysing samples of raw donkey and cow milk autoclave-treated (121°C for 15 min). Finally, the assay when applied to milk samples collected from the retail trade has confirmed the species indicated in the label. Furthermore, the assay represents a potentially valuable diagnostic tool for species identification in dairy products for allergic people.

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“…In 2007, an estimated 19,500 Mg of game meat was marketed, corresponding roughly to half the production volume of goats (FAO 2010). South African game meat has been exported for almost a century and is popular in Europe (Field 2004;Patterson and Khosa 2005;Mostert and Hoffman 2007;La Neve et al 2008). It is estimated that in 2005, South Africa exported the deboned meat of 160,000 game animal carcasses (Hoffman and Wiklund 2006).…”

Section: Introductionmentioning

confidence: 99%

Improving food security and safety through use of edible by-products from wild game

McCrindle

Siegmund‐Schultze

Heeb

et al. 2013

Environ Dev Sustain

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Game harvesting in South Africa generally leaves behind edible by-products, which could be a low-cost source of protein for poor people in rural areas. Seven professional and nine recreational hunters were interviewed, a game harvest and trophy hunt attended and literature reviewed, in order to describe the food value chain for game and ways in which edible by-products could be legally channelled into the human food chain, rather than being left in the field for scavengers. Practices of informal vendors (n = 51) were assessed using structured interviews, observation and microbiological analysis. In an experiment, inspected game by-products (shinbones from impala and springbok) were provided to eight informal traders for cooking at an informal market and microbiological analysis was done before and after preparation. The results showed that providing edible by-products to poor consumers appears to be culturally acceptable, affordable, accessible and safe. A crossover from formal to informal marketing is recommended as it would enhance traceability and safety of the product and minimise the risk of poaching. It is suggested that methods should be developed which make the distribution of edible byproducts to vulnerable rural communities feasible.

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Authentication of meat from game and domestic species by SNaPshot minisequencing analysis

Cited by 43 publications

References 20 publications

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Development of a real-time PCR assay for the detection of cow and donkey milk

Improving food security and safety through use of edible by-products from wild game

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