Identification of the secreted watery saliva proteins of the rice brown planthopper, Nilaparvata lugens (Stål) by transcriptome and Shotgun LC–MS/MS approach

Liu, Xiaoqing; Zhou, Hui; Zhao, Jing; Hua, Hongxia; He, Yueping

doi:10.1016/j.jinsphys.2016.04.002

Cited by 51 publications

(72 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some repeated amino acid sequences, a typical feature of mucin-like proteins (Verma and Davidson, 1994), were found. NlMLP protein has been detected in both gelling and watery saliva Liu et al, 2016). To investigate the functions of NlMLP, we analyzed mRNA levels in BPHs at various developmental stages, including eggs, first to fifth instar BPHs, and female and male adults, via quantitative reverse transcription (qRT)-PCR.…”

Section: Nlmlp Is Highly Expressed In N Lugens Salivary Glands and Smentioning

confidence: 99%

“…Like other piercing-sucking insects, BPHs secrete gelling and watery saliva. Recently, genomic tools such as proteomics and transcriptomics have been used to investigate BPH salivary glands and saliva at the molecular level (Konishi et al, 2009;Ji et al, 2013;Huang et al, 2016;Liu et al, 2016). Two secretary proteins that actively participate in salivary sheath formation were recently identified in BPHs (Huang et al, 2015.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants

et al. 2017

View full text Add to dashboard Cite

The brown planthopper, Nilaparvata lugens, is a pest that threatens rice (Oryza sativa) production worldwide. While feeding on rice plants, planthoppers secrete saliva, which plays crucial roles in nutrient ingestion and modulating plant defense responses, although the specific functions of salivary proteins remain largely unknown. We identified an N. lugens-secreted mucin-like protein (NlMLP) by transcriptome and proteome analyses and characterized its function, both in brown planthopper and in plants. NlMLP is highly expressed in salivary glands and is secreted into rice during feeding. Inhibition of NlMLP expression in planthoppers disturbs the formation of salivary sheaths, thereby reducing their performance. In plants, NlMLP induces cell death, the expression of defense-related genes, and callose deposition. These defense responses are related to Ca 2+ mobilization and the MEK2 MAP kinase and jasmonic acid signaling pathways. The active region of NlMLP that elicits plant responses is located in its carboxyl terminus. Our work provides a detailed characterization of a salivary protein from a piercing-sucking insect other than aphids. Our finding that the protein functions in plant immune responses offers new insights into the mechanism underlying interactions between plants and herbivorous insects.

show abstract

Section: Nlmlp Is Highly Expressed In N Lugens Salivary Glands and Smentioning

confidence: 99%

mentioning

confidence: 99%

A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Here, we reveal that the planthopper saliva can degrade DNA, and that the responsible enzyme is DNase II. In a previous study, the presence of salivary DNase II has been reported in the white‐backed planthopper (Huang et al ., ; Miao et al ., ), brown planthopper (Liu et al ., ; Huang et al ., ), and SBPH (Huang et al ., ). Although the composition of the saliva has been reported in > 10 Aphididae species, no salivary DNase II was found in these phloem‐feeding aphids (van Bel & Will, ), which deserved further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…In Drosophila melanogaster , DNase II is thought to have roles similar to those in C. elegans (Evans et al ., ). Surprisingly, DNase IIs have been found to be abundantly and ubiquitously distributed in the saliva of three planthoppers (Liu et al ., ; Huang et al ., ; Miao et al ., ) and two spider mite species (Huang et al ., ; Zhu et al ., ). However, the precise functions of these salivary DNase IIs remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

et al. 2019

View full text Add to dashboard Cite

Summary Extracellular DNA, released by damaged plant cells, acts as a damage‐associated molecular pattern (DAMP). We demonstrated previously that the small brown planthopper (Laodelphax striatellus, SBPH) secreted DNase II when feeding on artificial diets. However, the function of DNase II in insect feeding remained elusive. The influences of DNase II on SBPHs and rice plants were investigated by suppressing expression of DNase II or by application of heterogeneously expressed DNase II. We demonstrated that DNase II is mainly expressed in the salivary gland and is responsible for DNA‐degrading activity of saliva. Knocking down the expression of DNase II resulted in decreased performance of SBPH reared on rice plants. The dsDNase II‐treated SBPH did not influenced jasmonic acid (JA), salicylic acid (SA), ethylene (ET) pathways, but elicited a higher level of H2O2 and callose accumulation. Application of heterogeneously expressed DNase II in DNase II‐deficient saliva slightly reduced the wound‐induced defence response. We propose a DNase II‐based invading model for SBPH feeding on host plants, and provide a potential target for pest management.

show abstract

“…Thus, various salivary components may play important roles in feeding on host plants [5]. To date, extensive data have accumulated on the salivary gland transcriptome and proteome of vascular tissue-feeding hemipteran species, including the transcriptomes of Empoasca fabae Harris [6] and Nilaparvata lugens (Stål) [7] and the proteome of Acyrthosiphon pisum (Harris) [8, 9]. Although numerous salivary genes and proteins have been identified, functionally, only a few of them have been characterized [10].…”

Section: Introductionmentioning

confidence: 99%

The green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), salivary protein NcSP75 is a key effector for successful phloem ingestion

Matsumoto

Hattori

2018

PLoS ONE

View full text Add to dashboard Cite

Nephotettix cincticeps, a prevalent rice pest, injects gelling and watery saliva into plant tissues during the sucking process. Certain components within the saliva are believed to interact with plant cellular constituents and play important roles in overcoming host plant defense responses. Based on our previous analysis of the salivary gland transcriptome and secreted saliva proteome of N. cincticeps, in this study, we analyzed the biological functions of salivary protein, NcSP75 (N. cincticeps salivary protein 75 kD). NcSP75, a salivary glands-specific gene, showed low similarities to any previously reported sequences. Knockdown of NcSP75 by RNA interference (RNAi) reduced the longevity of treated nymphs to approximately half of the longevity of controls and caused severe developmental retardation. Furthermore, the knockdown of NcSP75 decreased the survival rate of adults, and reduced the number of deposited eggs and hatched nymphs. Thus, the adverse effects caused by the knockdown of NcSP75 were observed throughout the lifetime of N. cincticeps, when feeding on rice plants. In contrast, no reduction was observed in the survival rate of the knockdown of NcSP75 adults fed on an artificial diet. Electrical penetration graph measurements taken from adult females feeding on rice plants showed a significantly shorter duration of phloem ingestion associated with the knockdown of NcSP75 than the knockdown of the enhanced green fluorescent protein (EGFP). Furthermore, the total sugar content of the honeydew was lower when NcSP75 was knocked down. These results suggest that the NcSP75 protein contribute to successful and sustainable ingestion from the sieve elements of rice plants. The NcSP75 protein of N. cincticeps can, accordingly, be considered as a key effector for establishing compatible interaction with rice plants and could be a potential target for controlling this species.

show abstract

Identification of the secreted watery saliva proteins of the rice brown planthopper, Nilaparvata lugens (Stål) by transcriptome and Shotgun LC–MS/MS approach

Cited by 51 publications

References 69 publications

A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants

A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants

Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

The green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), salivary protein NcSP75 is a key effector for successful phloem ingestion

Contact Info

Product

Resources

About