Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection

Kotrebai, Mihály; Tyson, Julian F.; Uden, Peter C.; Birringer, Marc; Block, Eric

doi:10.1039/a902770j

Cited by 109 publications

(106 citation statements)

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“…The major organoselenium species in selenized yeast is selenomethionine [38][39][40]. In 2004, we reported [51] the presence of a sulfur-selenium amino acid, S-(methylseleno)cysteine, in archived selenized yeast samples from the Clark study [42].…”

Section: Resultsmentioning

confidence: 99%

“…Baker's yeast (Saccharomyces cerevisiae) is most commonly used for the production of supplements; selenized or 'high selenium yeasts' are prepared by growing yeast in a sodium selenite-enriched medium [35][36][37]. The principal organoselenium species produced in selenized yeast is selenomethionine [38,39], but selenoanions and other selenoamino acids including selenocystine, Se-lanthionine and Se-(methyl)selenocysteine have been determined at levels <1% of that of selenomethionine [40]. Ouerdane and Mester have reported essentially quantitative replacement of methionine by selenomethionine in yeast grown on a SeMet-containing medium [41].…”

Section: Introductionmentioning

confidence: 99%

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Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Amoako

Uden

Tyson

2009

Analytica Chimica Acta

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a b s t r a c tSpeciation of selenium is of interest because it is both essential and toxic to humans, depending on the species and the amount ingested. Following indications that selenium supplementation could reduce the incidence of some cancers, selenium-enriched yeast and other materials have been commercialized as supplements. Most dramatically however, the SELECT trial that utilized l-selenomethionine as the active supplement was terminated in 2008 and there is much debate regarding both the planning and the results of efficacy studies. Further, since dietary supplements are not regulated as pharmaceuticals, there are concerns about the quality, storage conditions, stability and selenium content in selenium supplements. Enzymatic hydrolysis enabled selenium speciation profiles to be obtained by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and following derivatization gas chromatography with atomic emission detection (GC-AED). Coated fiber solid phase microextraction (SPME) was used to extract volatile selenium species for determination by GC-AED and GC-MS. Similar speciation patterns were observed between yeast-based supplements subject to extended storage and those heated briefly at elevated temperatures. All the yeast-based supplements and one yeast-free supplement formed S-(methylseleno)cysteine on heating. Evidence was obtained in support of the hypotheses that S-(methylseleno)cysteine is formed from a reaction between dimethyldiselenide and cysteine or cystine.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Amoako

Uden

Tyson

2009

Analytica Chimica Acta

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“…Many Se-enriched plants and yeast have been developed, and major selenocompounds containing these biological materials are as follows: garlic, γ -glutamyl-Se-methylselenocysteine; onion, MeSeCys; broccoli sprouts, MeSeCys; leeks, MeSeCys; and yeast, SeMet (Cai et al 1995;Guo and Wu 1998;Kotrebai et al 1999;Ip et al 2000;Whanger 2002). Contents of MeSeCys are as follows: grassland legume, 10-13%; garlic, 3%; onion, 42-55%; broccoli sprout, 45%; leeks, 35-50%; and yeast, 6-20%.…”

Section: Discussionmentioning

confidence: 99%

Chemopreventive Effect of Selenium-Enriched Japanese Radish Sprout against Breast Cancer Induced by 7,12-Dimethylbenz[a]anthracene in Rats

Yamanoshita

Ichihara

Hama

et al. 2007

Tohoku J. Exp. Med.

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M., KAMIJIMA, M., TAKEDA, I. and NAKAJIMA, T. Chemopreventive Effect of anthracene in Rats. Tohoku J. Exp. Med., 2007, 212 (2), 191-198 Breast cancer is one of the major cancers in women, and dietary intake must be controlled to prevent it. Selenium (Se), especially Se compound in vegetables, is thought to be a promising chemopreventive dietary ingredient for preventing breast cancer. In this study, we developed Se-enriched Japanese radish sprout using a special Se-additional fertilizer, and identified the Se chemical forms. The newly developed Se-enriched sprout is produced within a week by the tank forming method, and the major chemical form was identified as Se-methylselenocysteine (MeSeCys) (80%). Then, the chemopreventive effects of the Se-enriched sprout were investigated using Sprague-Dawley female rats with mammary cancer, induced by a single oral dose of 10 mg or 14 mg of 7, 12-dimethylbenz[a]anthracene (DMBA). Mammary tumors were found in 11, 16 and 2 rats treated with DMBA and thereafter fed the basal (n = 34), sproutadded basal (n = 30) and Se-enriched sprout-added test diets (n = 30), respectively. The incidence of mammary tumors was significantly lower in the Se-enriched sprout-added test diet group (7%) than in the basal diet group (32%) or sprout-added basal diet group (53%). In contrast, no significant difference was detected in the numbers and incidence of the tumor between the basal diet group and Se-enriched sprout-added test diet group before DMBA-dosing. These results suggest that the diet supplement of Se-enriched sprout after DMBA-dosing provides a significant chemoprevention against chemical-induced mammary cancer. Thus, Se-enriched sprout may be a useful dietary ingredient for preventing breast cancer.

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“…Se exerts its biologic functions largely through its presence in selenoproteins and is known to be incorporated into proteins as SeCys and SeMet. In commercial Seenriched yeast, SeMet is the major selenocompound (Kotrebai et al, 1999). Calomme et al (1995) showed that Se was generally incorporated as SeCys into protein of L. delbrueckii subsp.…”

Section: Identification Of Selenium Speciation In Se-b Longummentioning

confidence: 99%

Preparation of Selenium-enriched Bifidobacterium Longum and its Effect on Tumor Growth and Immune Function of Tumor-Bearing Mice

Wang¹,

Wang²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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In this study, we demonstrated selenium (Se) accumulation in Bifidobacterium longum strain (B. longum) and evaluated the effect of Se-enriched B. longum (Se-B. longum) on tumor growth and immune function in tumor-bearing mice. Analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) revealed that more than 99% of Se in Se-B. longum was organic, the main component of which was selenomethionine (SeMet). In the in vivo experiments, tumor-bearing mice (n=8) were orally administrated with different doses of Se-B. longum alone or combined with cyclophosphamide (CTX). The results showed that the middle and high dose of Se-B. longum significantly inhibited tumor growth. When Se-B. longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time of tumor-bearing mice (n=12) was prolonged. Furthermore, compared with CTX alone, the combination of Se-B. longum and CTX stimulated the activity of natural killer (NK) cells and T lymphocytes, increasing the levels of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α), and the leukocyte count of H22 tumor-bearing mice (n=12).

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Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection

Cited by 109 publications

References 10 publications

Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Speciation of selenium dietary supplements; formation of S-(methylseleno)cysteine and other selenium compounds

Chemopreventive Effect of Selenium-Enriched Japanese Radish Sprout against Breast Cancer Induced by 7,12-Dimethylbenz[a]anthracene in Rats

Preparation of Selenium-enriched Bifidobacterium Longum and its Effect on Tumor Growth and Immune Function of Tumor-Bearing Mice

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