Identification of the Phytocomponents in Cymbopogon citratus Methanol Extract by Gas Chromatography-Mass Spectrometry

Igwe, K. K.; Udeh, Nkeiruka E.; Madubuike, A J; Akomas, SC; Amuneke, C. C.

doi:10.17722/ijrbt.v8i2.446

Cited by 4 publications

(3 citation statements)

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“…The identified phytochemicals including; nhexadecanoic acid, methyl ester (palmitic acid), hexanoic acid, vitamin E, oleic acid, phytol acetate, acetic acid and so on have been implicated for their antioxidant and other pharmacological activities [24;25].These findings have several differences and similarities in the nature and number of phytocompounds identified through GC-MS analysis of the leaf extract and fractions of T. occidentalis. Some of the major compounds are in tandem with the work of Ezekwe [26] which reported the presence of hexanoic acid (methyl ester) etc via GC-MS analysis of methanol extract of T. occidentalis; similarly, Igwe et al, [27] reported the presence of phytocompounds such as n-hexadecanoic acid (palmitic acid), octadecanoic acid (stearic acid), linoleic acid and so on in the GC-MS analysis of methanol extract of T. occidentalis. Most of the major compounds are uniquely different from the previous GC-MS analysis performed on T. occidentalis extract in literature, this may be due to the difference in age of the plant, concentration of ethanol (solvent) and extraction time [28].…”

Section: Discussionsupporting

confidence: 54%

Antioxidant activity and identification of bioactive compounds in Telfairia occidentalis leaves using GC-MS analysis

Christopher¹,

Willie²,

Akpan³

et al. 2022

World J. Adv. Res. Rev.

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Telfairia occidentalis belongs to the family cucurbitaceae. It is a plant with dark green leaves that also bears a pod containing edible seeds. It is widely cultivated in Western Africa, having high commercial importance in the eastern part of Nigeria. It is primarily used in the preparation of soup in the form of vegetable and in herbal medicines for production of blood tonic for weak and ill person, and also for the treatment of other ailments. Telfairia occidentalis leaves extract and fractions were evaluated for its phytochemical constituents, antioxidant activities and GC-MS analysis on the active extract and fractions to identify the bioactive compounds conferring these activities using standard procedures. The phytochemical screening of the ethanol extract and different fractions revealed the presence of flavonoids, alkaloids, tannins, saponin, cardiac glycoside and anthraquinones. The antioxidant activity was carried out using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) model; among the fractions ethyl acetate fraction (84.91%;100 µg/mL) demonstrated the best antioxidant activities in DPPH assay followed by the extract (80%;100 µg/mL), dichloromethane(79.51%;100 µg/mL) and n-hexane fractions (46.63%;100 µg/mL) with IC50 of 22.56 µg/mL, 37.43 µg/mL, 37.59 µg/mL, and 91.11 µg/mL. Ferric reducing antioxidant power (FRAP) recorded highest activity at 100 µg/mL with ethyl acetate having the highest antioxidant effect (1.574 nm) compared to ascorbic acid (1.750 nm; 100 µg/mL) followed by DCM fraction (1.040 nm), crude extract (0.966 nm), and n-hexane fraction (0.724 nm). The GC-MS analysis recorded a total of 39 compounds from extract; 9 major constituents; oleic acid, (10.27%), acetic acid (9.66%), hexanoic acid (7.66%), phytol acetate (7.22%), 18,19-seccoyohimban-19-oic acid (6.69%), iso-propyl 9,12,15-octadecatrienoate(6.20%), n-hexadecanoic acid, methyl ester (5.83%), trans-geranyl geraniol (4.21%) and 9,12- octadecadienoic acid (4.11%),39 compounds from DCM with 8 major constituents; (1,1-bicyclopropyl)-2-octanoic acid (25.87%), quinic acid (9%), acetic acid (6.66 %), 9-octadecadienoic acid (z,z) (5.63 %), benzofuran (5.34%), oxirane (4.11%), phytol, acetate (2.52 %) and vitamin E (2.37%), and 37 compounds from ethyl acetate fraction with 7 major constituents; stigmasterol (17.09%), vitamin E (14.73%), stigmasterol (11.25%), 4,22-stigmastadiene-3-one (9.40%), 7,22-ergostadienol (5.84%), dotricontane (3.88%) and eicosane (3.07%). From these results T. occidentalis extract and fractions shows high antioxidant activity. These compounds present in T. occidentalis extract and fractions could provide a rationale for the ethnomedicinal use of the plant in the management of several pathological conditions associated with oxidative stress including type 2 diabetes and inflammatory conditions.

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Section: Discussionsupporting

confidence: 54%

Antioxidant activity and identification of bioactive compounds in Telfairia occidentalis leaves using GC-MS analysis

Christopher¹,

Willie²,

Akpan³

et al. 2022

World J. Adv. Res. Rev.

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“…The GC-MS analysis of the BRARP extract detected a number of interesting compounds. Some of these compounds have earlier been reported to possess anticancer, antioxidant and antimicrobial potentials ( Table 4 ) [ 44 – 53 ]. The present study further found the antimicrobial activity of commercially available identified compounds and compared.…”

Section: Discussionmentioning

confidence: 99%

Preservative effect of Chinese cabbage (Brassica rapa subsp. pekinensis) extract on their molecular docking, antioxidant and antimicrobial properties

Chellia

Saravanakumar

et al. 2018

PLoS ONE

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This study aimed at investigating the antimicrobial activity of different solvent extracts of Chinese cabbage Brassica rapa subsp. pekinensis (BRARP) and their antioxidant and cytotoxicity properties. Of the different solvents extracts, the chloroform extracts (CE) were significantly inhibited the bacterial pathogens at minimum inhibitory concentration (MIC) of 16.5 mg.mL-1. Biochemical analysis revealed that total phenol (62.6 ± 0.05 mg GAE.g-1) and flavonoids (27.6 ± 0.04 mg QE.g-1) were higher in the extracts of BRARP, which resulted in enhanced antioxidant activity in CE. A total of eight dominant compounds were detected in the potent antimicrobial extract from BRARP based on GC-MS analysis. The molecular interactions study revealed that, among the screened compounds the 1,2-benzenedicarboxylic acid and 2,3-dicyanopropionamide interacted with the active site of pathogenicity and survival related protein with lipopolysaccharide (LpxC) with higer binding energy. This work concluded that the 1, 2-Benzenedicarboxylic acid and 2, 3-Dicyanopropionamide from BRARP was reported to be good non-cytotoxic and antioxidant antimicrobials against bacterial pathogens.

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“…6 Spectroscopic methods have been used to determine the structures of plant extracts. [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] More research is needed to elucidate the structures of this plant. It's often not clear how its bioactivity and its phytochemical components are related.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Tertiary Substituted Amine from the Leaves of Sarcophrynium brachystachys (Benth.) K Schum

2023

TJNPR

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Sarcophrynium brachyatachys, commonly known as Yoruba soft cane or the praying plant, is traditionally used for packaging food items. Only a few natives recognize the usefulness of the leaves for the treatment of cough and bronchitis in herbal medicine. This study was designed to isolate and characterize a bioactive component. Successful percolation was carried out with 96 percent ethanol to get the crude extract. Afterwards, fractionation was carried out using chloroform and distilled water in a ratio of 1:1. The chloroform fraction was further fractionated with methanol and n-hexane. Column chromatography was carried out on the methanol portion of the extract with organic solvents of different polarities to isolate a pure compound, which was ascertained by thin layer chromatography. The isolated component was characterized based on FT-IR, 1 H and 13 C NMR spectra, DEPT-135, HSQC, COSY and mass spectrometry. FTIR spectrum revealed a weakly intense peak at 3100 cm -1 , which is typical of aromatic C-H. The 1 HNMR spectrum of showed the chemical shift of six member ring aromatic protons as doublets at 7.23 ppm (1H, d), 7.27 ppm (1H, d), and 7.32 ppm (1H, d). The methine tertiary amine proton signal was observed at 3.26 ppm. In the 13 CNMR spectrum, the tertiary methine amine carbon was observed at 62.62 ppm, while the tertiary methyl amine carbon resonated at 56.87 ppm. The isolated compound was identified as a tertiary substituted amine with the name 5-(2-(secbutyl)phenoxy)-N-(1-ethoxypropyl)-6-(2-isobuthyl-2,3-dimetylcyclopropoxy)-N-methyldec-1en-2-amine.

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Identification of the Phytocomponents in Cymbopogon citratus Methanol Extract by Gas Chromatography-Mass Spectrometry

Cited by 4 publications

References 0 publications

Antioxidant activity and identification of bioactive compounds in Telfairia occidentalis leaves using GC-MS analysis

Antioxidant activity and identification of bioactive compounds in Telfairia occidentalis leaves using GC-MS analysis

Preservative effect of Chinese cabbage (Brassica rapa subsp. pekinensis) extract on their molecular docking, antioxidant and antimicrobial properties

Isolation and Characterization of a Novel Tertiary Substituted Amine from the Leaves of Sarcophrynium brachystachys (Benth.) K Schum

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