Preservative effect of Chinese cabbage (Brassica rapa subsp. pekinensis) extract on their molecular docking, antioxidant and antimicrobial properties

Abstract

This study aimed at investigating the antimicrobial activity of different solvent extracts of Chinese cabbage Brassica rapa subsp. pekinensis (BRARP) and their antioxidant and cytotoxicity properties. Of the different solvents extracts, the chloroform extracts (CE) were significantly inhibited the bacterial pathogens at minimum inhibitory concentration (MIC) of 16.5 mg.mL-1. Biochemical analysis revealed that total phenol (62.6 ± 0.05 mg GAE.g-1) and flavonoids (27.6 ± 0.04 mg QE.g-1) were higher in the extracts of BRARP, which resulted in enhanced antioxidant activity in CE. A total of eight dominant compounds were detected in the potent antimicrobial extract from BRARP based on GC-MS analysis. The molecular interactions study revealed that, among the screened compounds the 1,2-benzenedicarboxylic acid and 2,3-dicyanopropionamide interacted with the active site of pathogenicity and survival related protein with lipopolysaccharide (LpxC) with higer binding energy. This work concluded that the 1, 2-Benzenedicarboxylic acid and 2, 3-Dicyanopropionamide from BRARP was reported to be good non-cytotoxic and antioxidant antimicrobials against bacterial pathogens.

Potential application of Brassica rapa subsp. pekinensis extract on fresh beef meat during refrigeration storage

“…The inhibition activity of BRARP extract significantly increased ( p < .05) with an increase in the concentration of BRARP until 2%; however, there were as no significant difference between the fractions 2% and 2.5% ( p < .05). The results for antimicrobial activity of the BRARP extract which are indicated in Table confirm the results of previous studies, which reported that BRARAP possesses antimicrobial activity (Jarriyawattanachaikul, Chaveerach, & Chokesajjawatee, ; Patra & Baek, ; Rubab et al, ). In addition, there was no significant difference between the inhibition activities of 2% and 2.5% fraction against the targeted pathogens.…”

“…The corresponding changes with and without BRARP extract had no significant difference ( p < .05) throughout the storage period. This could be attributed to the antioxidant potential of BRARP extract as reported previously (Rubab et al, ). However, the measured L* values show a statistically significant difference between the control and BRARP‐treated samples during storage, these effects on lightness were subjectively negligible.…”

“…Natural antimicrobial agents as an alternative preservative to replace the commercially available chemical preservatives to ensure Table 1 confirm the results of previous studies, which reported that BRARAP possesses antimicrobial activity (Jarriyawattanachaikul, Chaveerach, & Chokesajjawatee, 2016;Patra & Baek, 2017;Rubab et al, 2018). In addition, there was no significant difference between the inhibition activities of 2% and 2.5% fraction against the targeted pathogens.…”

“…According to GC‐MS analysis, a total of six compounds have been identified (Table ). Among the identified compounds, some of these previously reported for their antimicrobial activity (Rubab et al, ). Further computer‐based in silico docking method was performed to validate and understand the evidence for observed antimicrobial activity of identified compounds in GC‐MS analysis.…”

“…According to GC-MS analysis, a total of six compounds have been identified (Table 2). Among the identified compounds, some of these previously reported for their antimicrobial activity (Rubab et al, 2018).…”