Identification of the novel HLA‐B*27:147 allele by polymerase chain reaction sequence‐based typing

Abstract

HLA-B*27:147 differs from HLA-B*27:04:01 by a single nucleotide substitution at position 523 C>G.

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians

“…The allele frequency of ITGAM*01 (95.5%) found in the present study is higher than that found among the Germans, Caucasians, and Turkish, but it is slightly lower than that seen among the other Asian groups such as Japanese, Thais, and Chinese (Table 2). The genotype IT-GAM*01+*02− (HNA-4a homozygous) was found to be the most common genotype in all the previous studies [22,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. This suggests that antibodies to HNA-4a may also be involved in Indian patients with NAN and AIN as reported previously in other studies [40].…”

“…This suggests that antibodies to HNA-4a may also be involved in Indian patients with NAN and AIN as reported previously in other studies [40]. The frequency of HNA-5a i.e., ITGAL*01 (23.7%) found in Indians is significantly lower (p < 0.05) as compared to that seen among all other populations worldwide (range: 50-89.6%) (Table 2) [22,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. These data confirm that the chances of alloimmunization and immune neutropenia due to anti-HNA-5a in the Indian population will occur more frequently as compared to any other population worldwide.…”

“…Frequency of FCGR3B*02 was comparable to Chinese and Thais but was higher than Japanese and lower than Caucasians, Germans, Brazilians, and Turkish [22, 26, 30, 33, 35-37, 39, 42]. Frequency of FCGR3B*03 (8.76%) was highest as compared to all other populations [22,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] except Zambians. This may be due to the ethnic differences among both population groups and/or sample size variation.…”

“…S1). These were analysed by direct DNA sequencing as described by He et al [34]. To further differentiate between presence of both or any of the two alleles (FCGR3B*02 and FCGR3B*03), PCR-RFLP with SfaNI restriction enzyme (Thermo Fisher Scientific, USA) was carried out as recommended by Steffensen et al [46].…”

Identification of the novel HLA‐B*27:04:06 allele in a Chinese bone marrow donor

“…To further confirm the alleles of HLA‐B locus in the sample, the HLA‐B*15 and HLA‐B*27 alleles were amplified with the allele‐specific primers separately as documented in our previous reports . The sequencing results showed HLA‐B alleles of the sample as HLA‐B*15:11 and a novel allele.…”