Identification of the metaphyseal skeletal stem cell building trabecular bone

Yang, Guan; He, Qi; Guo, Xiaoxiao; Li, Rong-Yu; Lin, Jingting; Lang, Yiming; Tao, Wanyu; Liu, Wenjia; Lin, Huisang; Xing, Shilai; Qi, Yini; Xie, Zhongliang; Han, Jing-Dong J.; Zhou, Bin; Teng, Yan; Yang, Xiao

doi:10.1126/sciadv.adl2238

Cited by 3 publications

(2 citation statements)

References 58 publications

(92 reference statements)

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“…31 Recent studies reported LIFR + PDGFRB + CD45 − CD31 − CD235a − MSCs and Pdgfra + Lepr + marrow-associated skeletal stem and progenitor cells could serve as the identification marker of BMSCs in vivo. 32,33 BMSCs exhibit characteristics such as colony-forming ability, self-renewal capacity, and multilineage differentiation potential along mesodermal lineages, enabling the development of various tissues and organs, including bone, AC, and adipose tissue, etc. 33,34 Currently, BMSCs serve as primary cell sources for endogenous AC and OC tissue repair.…”

Section: Cell Sources For Endogenous Ac and Oc Tissue Engineeringmentioning

confidence: 99%

“…The BMSCs are primarily located within the bone marrow and exhibit positive expression of typical cell surface markers, including CD29, CD44, CD73, CD90, CD105, CD146, and CD271. , Conversely, BMSCs typically exhibit negative expression of common cell surface markers, including CD45, CD34, CD14, CD11b, and CD19 . Recent studies reported LIFR + PDGFRB + CD45 – CD31 – CD235a – MSCs and Pdgfra + Lepr + marrow-associated skeletal stem and progenitor cells could serve as the identification marker of BMSCs in vivo. , BMSCs exhibit characteristics such as colony-forming ability, self-renewal capacity, and multilineage differentiation potential along mesodermal lineages, enabling the development of various tissues and organs, including bone, AC, and adipose tissue, etc. , Currently, BMSCs serve as primary cell sources for endogenous AC and OC tissue repair. However, their application is associated with an increased tendency for fibrocartilage formation and ectopic ossification, possibly due to their inherent high osteogenic potential. , Furthermore, in cases of partial-thickness and full-thickness AC injuries where the subchondral bone remains intact, additional procedures such as MF or subchondral drilling are necessary to access endogenous BMSCs .…”

Section: Cell Sources For Endogenous Ac and Oc Tissue Engineeringmentioning

confidence: 99%

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Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Zhang,

Chen,

Sun

et al. 2024

ACS Biomater. Sci. Eng.

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Increasing attention has been paid to the development of effective strategies for articular cartilage (AC) and osteochondral (OC) regeneration due to their limited selfreparative capacities and the shortage of timely and appropriate clinical treatments. Traditional cell-dependent tissue engineering faces various challenges such as restricted cell sources, phenotypic alterations, and immune rejection. In contrast, endogenous tissue engineering represents a promising alternative, leveraging acellular biomaterials to guide endogenous cells to the injury site and stimulate their intrinsic regenerative potential. This review provides a comprehensive overview of recent advancements in endogenous tissue engineering strategies for AC and OC regeneration, with a focus on the tissue engineering triad comprising endogenous stem/ progenitor cells (ESPCs), scaffolds, and biomolecules. Multiple types of ESPCs present within the AC and OC microenvironment, including bone marrow-derived mesenchymal stem cells (BMSCs), adipose-derived mesenchymal stem cells (AD-MSCs), synovial membrane-derived mesenchymal stem cells (SM-MSCs), and AC-derived stem/progenitor cells (CSPCs), exhibit the ability to migrate toward injury sites and demonstrate pro-regenerative properties. The fabrication and characteristics of scaffolds in various formats including hydrogels, porous sponges, electrospun fibers, particles, films, multilayer scaffolds, bioceramics, and bioglass, highlighting their suitability for AC and OC repair, are systemically summarized. Furthermore, the review emphasizes the pivotal role of biomolecules in facilitating ESPCs migration, adhesion, chondrogenesis, osteogenesis, as well as regulating inflammation, aging, and hypertrophy-critical processes for endogenous AC and OC regeneration. Insights into the applications of endogenous tissue engineering strategies for in vivo AC and OC regeneration are provided along with a discussion on future perspectives to enhance regenerative outcomes.

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Section: Cell Sources For Endogenous Ac and Oc Tissue Engineeringmentioning

confidence: 99%

Section: Cell Sources For Endogenous Ac and Oc Tissue Engineeringmentioning

confidence: 99%

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Zhang,

Chen,

Sun

et al. 2024

ACS Biomater. Sci. Eng.

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Hydrolyzed egg yolk peptide prevented osteoporosis by regulating Wnt/β-catenin signaling pathway in ovariectomized rats

Chen,

Huang,

Chen

et al. 2024

Sci Rep

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Hydrolyzed egg yolk peptide (YPEP) was shown to increase bone mineral density in ovariectomized rats. However, the underlying mechanism of YPEP on osteoporosis has not been explored. Recent studies have shown that Wnt/β-catenin signaling pathway and gut microbiota may be involved in the regulation of bone metabolism and the progression of osteoporosis. The present study aimed to explore the preventive effect of the YPEP supplementation on osteoporosis in ovariectomized (OVX) rats and to verify whether YPEP can improve osteoporosis by regulating Wnt/β-catenin signaling pathway and gut microbiota. The experiment included five groups: sham surgery group (SHAM), ovariectomy group (OVX), 17-β estradiol group (E2: 25 µg /kg/d 17β-estradiol), OVX with low-dose YPEP group (LYPEP: 10 mg /kg/d YPEP) and OVX with high-dose YPEP group (HYPEP: 40 mg /kg/d YPEP). In this study, all the bone samples used were femurs. Micro-CT analysis revealed improvements in both bone mineral density (BMD) and microstructure by YPEP treatment. The three-point mechanical bending test indicated an enhancement in the biomechanical properties of the YPEP groups. The serum levels of bone alkaline phosphatase (BALP), bone gla protein (BGP), calcium (Ca), and phosphorus (P) were markedly higher in the YPEP groups than in the OVX group. The LYPEP group had markedly lower levels of alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide of type I collagen (CTX-I) than the OVX group. The YPEP groups had significantly higher protein levels of the Wnt3a, β-catenin, LRP5, RUNX2 and OPG of the Wnt/β-catenin signaling pathway compared with the OVX group. Compared to the OVX group, the ratio of OPG/RANKL was markedly higher in the LYPEP group. At the genus level, there was a significantly increase in relative abundance of Lachnospiraceae_NK4A136_group and a decrease in Escherichia_Shigella in YPEP groups, compared with the OVX group. However, in the correlation analysis, there was no correlation between these two bacteria and bone metabolism and microstructure indexes. These findings demonstrate that YPEP has the potential to improve osteoporosis, and the mechanism may be associated with its modulating effect on Wnt/β-catenin signaling pathway.

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Skeletal stem and progenitor cells in bone development and repair

Trompet,

Melis,

Chagin

et al. 2024

Journal of Bone and Mineral Research

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Bone development, growth, and repair are complex processes involving various cell types and interactions, with central roles played by skeletal stem and progenitor cells. Recent research brought new insights into the skeletal precursor populations that mediate intramembranous and endochondral bone development. Later in life, many of the cellular and molecular mechanisms determining development are reactivated upon fracture, with powerful trauma-induced signaling cues triggering a variety of postnatal skeletal stem/progenitor cells (SSPCs) residing near the bone defect. Interestingly, in this injury context, the current evidence suggests that the fates of both SSPCs and differentiated skeletal cells can be considerably flexible and dynamic, and that multiple cell sources can be activated to operate as functional progenitors generating chondrocytes and/or osteoblasts. The combined implementation of in vivo lineage tracing, cell surface marker-based selection, single-cell molecular analyses, and high-resolution in situ imaging has strongly improved our insights into the diversity and roles of developmental and reparative stem/progenitor subsets, while also unveiling the complexity of their dynamics, hierarchies, and relationships. Albeit incompletely understood at present, findings supporting lineage flexibility and possibly plasticity among sources of osteogenic cells challenge the classical dogma of a single primitive, self-renewing, multipotent stem cell driving bone tissue formation and regeneration from the apex of a hierarchical and strictly unidirectional differentiation tree. We here review the state of the field and the newest discoveries in the origin, identity, and fates of skeletal progenitor cells during bone development and growth, discuss the contributions of adult SSPC populations to fracture repair, and reflect on the dynamism and relationships among skeletal precursors and differentiated cell lineages. Further research directed at unraveling the heterogeneity and capacities of SSPCs, as well as the regulatory cues determining their fate and functioning, will offer vital new options for clinical translation toward compromised fracture healing and bone regenerative medicine.

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Identification of the metaphyseal skeletal stem cell building trabecular bone

Cited by 3 publications

References 58 publications

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Hydrolyzed egg yolk peptide prevented osteoporosis by regulating Wnt/β-catenin signaling pathway in ovariectomized rats

Skeletal stem and progenitor cells in bone development and repair

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