Identification of the Male-Produced Aggregation Pheromone of the Four-Spotted Coconut Weevil, <i>Diocalandra frumenti</i>

Vacas, Sandra; Navarro, Ismael; Seris, E.; Ramos, Carina Guedes; Hernández, E Hernández; Navarro‐Llopis, Vicente; Primo, Jaime

doi:10.1021/acs.jafc.6b04829

Cited by 6 publications

(3 citation statements)

References 21 publications

(36 reference statements)

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“…Mated females were sampled on the lemons from the main stock colony after checking for the presence of ovisacs. The production of sex pheromone was confirmed by collection of emitted volatiles from a group of 20−30 virgin females by solid phase microextraction technique (SPME) (Vacas et al, 2017). The identity of the sex pheromone was unequivocally confirmed by full coincidence of its mass spectra and retention time with an analytical standard sample synthesised according to the method described by Kukovinets et al, 2006.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterisation ofPlanococcus citri cis- andtrans-isoprenyl diphosphate synthase genes, supported by short- and long-read transcriptome data

Juteršek

Gerasymenko

Petek

et al. 2023

Preprint

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Many insect species rely on diverse terpenoids for their development and interorganismal interactions. However, little is known about terpenoid biosynthesis in insects. The monoterpenoid sex pheromones of mealybugs and scale insects (Coccoidea) are particularly enigmatic, with several species producing unique structures presumed to result from the irregular coupling activity of unidentified isopentenyl diphosphate synthases (IDSs). Enzymes capable of similar transformations have previously only been described from a few plant, bacterial and archaeal species. To investigate if insect irregular monoterpenes can be biosynthesised by similar enzymes, we performed a comprehensive search for IDS coding sequences in the genome ofPlanococcus citri, a widespread agricultural pest. We complemented the availableP. citrigenome data with newly generated short- and long-read transcriptome data. The identified candidate genes had homology to both short- and long-chain IDSs and some appeared to be paralogous, indicating gene duplications and consequent IDS gene family expansion inP. citri. We tested the activity of eleven candidate gene products, confirming in vitro regular activity for five enzymes, one of which (transIDS5) also produced the irregular prenyl diphosphates, maconelliyl and lavandulyl diphosphate. Targeted mutagenesis of selected aspartates and a lysine in the active site oftransIDS5 uncovered their importance for chain length preference and irregular coupling. This work provides an important foundation for deciphering terpenoid biosynthesis in mealybugs, as well as a potential source of enzymes for the biotechnological production of sustainable insect pest management products.

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Section: Methodsmentioning

confidence: 99%

Identification and characterisation ofPlanococcus citri cis- andtrans-isoprenyl diphosphate synthase genes, supported by short- and long-read transcriptome data

Juteršek

Gerasymenko

Petek

et al. 2023

Preprint

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“…They also concluded that this pest was not only affect on the young fruits, but also on the mature fruits. Report by Vacas et al (2017) also stated that this species is difficult to recognize with popular symptoms such as emergence holes, fruit fall, gum exudate.…”

Section: Attack and Damage Behavior Of Diocalandra Frumentimentioning

confidence: 99%

“…The European and Mediterranean Plant Protection Organization (EPPO 2012) reported that tts damages can cause fruit deformity and stunting. Larvae attack the tissues of trunks and leaves and causing gum exudation, yellowing of leaves (Vacas et al 2017). According to Vacas et al (2017), D. frumenti has been identified as an important pest in economically valuable palm species, including Date palm and Canary palm.…”

Section: Introductionmentioning

confidence: 99%

Biology, morphology and damage of the lesser Coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam

Nguyen

Chau

et al. 2020

Biodiversitas

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Abstract. Nguyen HU, Nguyen TH, Chau NQK, Le VV, Tran VH. 2020. Biology, morphology and damage of the lesser coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam. Biodiversitas 21: 4686-4694. The lesser coconut weevil, Diocalandra frumenti, is an emerging pest of coconut trees in Vietnam. To help develop control options for D. frumenti, this study investigated its morphological and biological characteristics, and quantified damage levels on coconut trees. Results from the study showed that attack by D. frumenti on coconut trees is correlated with characteristic damage symptoms (e.g., oozing sap) on all maturity stages of coconut fruits throughout the year (24.7% infestation), with higher damage levels on young fruits (57.6%). Results also showed that infestation levels on trees (58.9%), coconut bunches (19.4%), and fruits (7.77%) varied greatly. Adults have four different morphologies, but genetic study showed that they are all one species. The life cycle from egg to adult averaged 167± 34.3 days. The average development time for eggs, larvae, female pupae, male pupae, male adults, and female adults was 5.62 ± 0.62, 142.3 ± 34.3, 9.79 ± 0.86, 10.2 ± 1.26, 81.5 ± 34.7, and 81.8 ± 37.2 days, respectively. In conclusion, D. frumenti is an important pest in coconut trees with significant fluctuations in the duration of its development stages, and variation in shape, color, and size of the eggs, larvae, pupae, and adults. They can attack any coconut orchard and have harmful impacts on coconut trees, coconut bunches, and fruits throughout the year.

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Aggregation Pheromone of the Bearded Weevil, Rhinostomus barbirostris (Coleoptera: Curculionidae): Identification, Synthesis, Absolute Configuration and Bioactivity

et al. 2018

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The bearded weevil, Rhinostomus barbirostris (Coleoptera: Curculionidae: Dryophthorinae), attacks coconut trees, oil palms and other species of Arecaceae. Besides direct damage, R. barbirostris may be a vector of diseases in coconut and oil palms, such as stem bleeding (resinosis) and red ring disease. Currently, the only method to control this weevil is by visual observation of damage and removal of infected plants. Semiochemical-based trapping could improve the effectiveness of monitoring and management of R. barbirostris. In comparisons of volatiles released by R. barbirostris males and females by gas chromatography (GC) two male-specific compounds were observed. GC-mass spectrometry (MS) and GC-Fourier transform-infrared (FTIR) analyses of the natural compounds suggested these were diastereoisomers of 5-hydroxy-4-methylheptan-3-one, also known as sitophilure, a pheromone component of other dryophthorine species. Synthesis of the mixture of all four stereoisomers of sitophilure was performed in two steps, and the chemical structures were confirmed by comparing GC retention times and MS and FTIR spectra of natural and synthetic compounds. The absolute configurations of the two male-specific compounds were elucidated by enantioselective GC; the major component was the (4S,5R)-isomer, and the minor component (4S,5S)-sitophilure. In analyses by GC-electroantennography (EAG) the antennae of male and female R. barbirostris only responded to the (4S,5R)-isomer of the synthetic sitophilure. The stereoisomeric mixture of sitophilure was attractive to both sexes of R. barbirostris in laboratory experiments in the presence of sugar cane volatiles, and a similar result was obtained in a preliminary field trapping test.

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Identification of the Male-Produced Aggregation Pheromone of the Four-Spotted Coconut Weevil, Diocalandra frumenti

Abstract: 20The four-spotted coconut weevil, Diocalandra frumenti Fabricius (Coleoptera: 21 Curculionidae), is a small weevil found attacking economically important palm species

Cited by 6 publications

References 21 publications

Identification and characterisation ofPlanococcus citri cis- andtrans-isoprenyl diphosphate synthase genes, supported by short- and long-read transcriptome data

Identification and characterisation ofPlanococcus citri cis- andtrans-isoprenyl diphosphate synthase genes, supported by short- and long-read transcriptome data

Biology, morphology and damage of the lesser Coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam

Aggregation Pheromone of the Bearded Weevil, Rhinostomus barbirostris (Coleoptera: Curculionidae): Identification, Synthesis, Absolute Configuration and Bioactivity

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