2014
DOI: 10.1371/journal.pone.0087053
|View full text |Cite
|
Sign up to set email alerts
|

Identification of the Maize Gravitropism Gene lazy plant1 by a Transposon-Tagging Genome Resequencing Strategy

Abstract: Since their initial discovery, transposons have been widely used as mutagens for forward and reverse genetic screens in a range of organisms. The problems of high copy number and sequence divergence among related transposons have often limited the efficiency at which tagged genes can be identified. A method was developed to identity the locations of Mutator (Mu) transposons in the Zea mays genome using a simple enrichment method combined with genome resequencing to identify transposon junction fragments. The s… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
25
0
1

Year Published

2016
2016
2023
2023

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 26 publications
(26 citation statements)
references
References 63 publications
0
25
0
1
Order By: Relevance
“…A candidate sk1 gene was identified within this interval by the characterization of a second sk1 allele ( sk1-rMu1 ) derived from active Mutator ( Mu ) maize lines ( 15 ). We used Mu -Taq, a genome sequencing approach that enriches for Mu -chromosomal junction fragments ( 16 ). Of the 179 total independent Mu junction fragments identified, 2 mapped within the coding sequence of GRMZM2G021786, a predicted gene located within the sk1 genetic interval, making it a candidate for the sk1 gene (Fig.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…A candidate sk1 gene was identified within this interval by the characterization of a second sk1 allele ( sk1-rMu1 ) derived from active Mutator ( Mu ) maize lines ( 15 ). We used Mu -Taq, a genome sequencing approach that enriches for Mu -chromosomal junction fragments ( 16 ). Of the 179 total independent Mu junction fragments identified, 2 mapped within the coding sequence of GRMZM2G021786, a predicted gene located within the sk1 genetic interval, making it a candidate for the sk1 gene (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Taq α I libraries were created with genomic DNA extracted from four independent plants homozygous for the sk1-rMu1 allele. The custom adapters used for sk1-rMu1 cloning were of an earlier iteration than those described by Howard et al ( 16 ). One adapter incorporated a 4-bp barcode index, whereas the other was a common adapter (table S2).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The gene affected in a classic maize lazy mutant was recently identified and found to be a homolog of OsLAZY1 (Dong et al, 2013;Howard et al, 2014). Arabidopsis LAZY1 (AtLAZY1) is also required for gravitropism in the inflorescence stem and seedling hypocotyl (Yoshihara et al, 2013).…”
mentioning
confidence: 99%
“…LAZY1 was shown to be required for auxin redistribution in response to gravitropic stimulation in rice (Oryza sativa; Godbole et al, 1999;Li et al, 2007;Yoshihara and Iino, 2007) and in maize (Zea mays; Dong et al, 2013). OsLAZY1, ZmLAZY1, and AtLAZY1 localize to the nucleus and at the cell periphery, probably including the plasma membrane (Dong et al, 2013;Yoshihara et al, 2013;Howard et al, 2014;Sasaki and Yamamoto, 2015). The rice Deeper Rooting1 (OsDRO1) is also a LAZY family gene (Yoshihara et al, 2013).…”
mentioning
confidence: 99%