Identification of the main mechanisms involved in the tolerance and bioremediation of Cr(VI) by Bacillus sp. SFC 500-1E

Ontañon, Ornella Mailén; Fernández, Marilina; Agostini, Elizabeth; González, Pablo J.

doi:10.1007/s11356-018-1764-1

Cited by 50 publications

(21 citation statements)

References 54 publications

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“…Similarly, ArsR family transcriptional regulator was upregulated in the presence of arsenic and chromium [25,32]. Bacteria are known to tolerate arsenite and chromate due to the presence of efflux mechanisms [55,56]. However, no such efflux-specific proteins were detected in this study.…”

Section: Discussionmentioning

confidence: 52%

Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium—a proteomic approach

Shah

Damare

2020

Braz J Microbiol

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Cellular response against different heavy metal stress differs with the metal. Arsenic and chromium are heavy metals and toxic to living systems. The concentration of these metals in seawater is very low. However, due to their solubility in nature, they actively enter cells via various transport mechanisms and cause damage to the cells. Brevibacterium casei #NIOSBA88, a marine-derived, gram-positive isolate was multi-metal tolerant. Proteomic analysis of this isolate in response to arsenic and chromium resulted in the identification of total 2549 proteins, out of which 880 proteins were found to be commonly expressed at 750 mgL −1 arsenic and 100 mgL −1 chromium and in absence of both the metals. In contrast, 533, 212, and 270 proteins were found to be unique in the absence of any metal, 750 mgL −1 of arsenic and 100 mgL −1 of chromium respectively. Proteins such as antibiotic biosynthesis monooxygenase, ArsR family transcriptional regulator, cytochrome C oxidase subunit II, and thioredoxin reductase were exclusively expressed only in response to arsenic and chromium. Other proteins like superoxide dismutase, lipid hydroperoxide reductase, and thioredoxin-disulfide reductase were found to be upregulated in response to both the metals. Most of the proteins involved in the normal cell functioning were found to be downregulated. Major metabolic functions affected include amino acid metabolism, carbohydrate metabolism, translation, and energy metabolism. Peptide mass fingerprinting of Brevibacterium casei #NIOSBA88 exposed to arsenic and chromium respectively revealed the deleterious effect of these metals on the bacterium and its strategy to overcome the stress.

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Section: Discussionmentioning

confidence: 52%

Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium—a proteomic approach

Shah

Damare

2020

Braz J Microbiol

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“…strain KSUCr5 [23] ; B. subtilis [24] ; B.circulans [25] ; B.cereus [26] ; B.subtilis [17] ; B.methylotrophicus [18] ; Bacillus sp. SFC 500-1E [27] ; Bacillus sp. M6 [28] have been extensively studied for Cr bioremediation.…”

Section: Chrrs From Microbial Sourcesmentioning

confidence: 99%

“…Hexavalent chromate reduction by Aspergillus niger led to formation of Cr 3+ inside the fungal cells and on the mycelial surface by ChrR which was produced only intracellularly by assays of the enzyme activities in permeabilized cells, cell-free extracts and cell debris [38] . Ontanon et al [27] have studied that chromate reduction was mediated by a reductase enzyme (ChrR) in the soluble fraction of Bacillus sp. SFC 500-IE.…”

Section: Localization Of Chrrsmentioning

confidence: 99%

“…A Bacillus sp. SFC 500-1E has been isolated from a bacterial consortium SFC 500-1 which was obtained from tannery sediments and displayed tolerance to Cr [27] .…”

Section: Expression Of Chrrsmentioning

confidence: 99%

“…Growth-dependent Cr 6+ reduction was also observed in Geobacter sulfurreducens [63] . Ontanon et al [27] have suggested the chromate reduction through cytosolic NADH-dependent ChrR in a Bacillus sp. SFC 500-1E as the main mechanism for bioremediation of Cr 6+ .…”

Section: Mechanisms Of Chromate Reduction By Chromate Reductasesmentioning

confidence: 99%

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Microbial Chromate Reductases: Novel and Potent Mediators in Chromium Bioremediation-A Review

Mala¹,

Takeuchi²,

Mani³

2020

Applied Microbiology: Theory ＆ Technology

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Heavy metal pollution from the growing industrialization are a significant cause of environmental concern.Chromium (Cr) is commonly used in the production of stainless steel, textile dyeing, electroplating, as nuclear coolants and largely in chrome tanning of hides and skins. About 90% of leather is produced by chrome tanning and the leather industry contributes to an overload of Cr toxicity in tannery effluents. Accumulation of Cr6+ is carcinogenic, genotoxic and teratogenic to organisms. Biological methods are ‘green’ approaches for chromium bioremediation and microorganisms are the desired candidates for pollution abatement. Microbial chromate reduction is mediated by chromate reductases (ChrRs) which may be expressed constitutively or inducibly. ChrRs have been produced by a number of bacteria, fungi and yeasts and may be extracellular or localized in the membrane or cytosol. ChrRs are dependent on electron donors such as reduced Nicotinamide adenine dinucleotide (NADH) or reduced Nicotinamide adenine dinucleotide phosphate (NADPH) or reduced Glutathione (GSH) as cofactors. In chromate reduction by ChrRs, Cr6+ undergo one electron transfer to producean unstable Cr5+ radical that is converted to stable and less toxic Cr3+. Putative ChrR genomic sequences have been studied with 99% sequence similarity in Gram negative bacteria. ChrRs are valuable resources in different environments for chromate reduction. This review is to discuss the expression and characteristics of ChrRs and their mechanisms in reduction of Cr6+ toxicity in order to provide a comprehensive understanding of this novel class of enzymes for promisingapplications in Cr bioremediation.

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Morphological and structural response of Bacillus sp. SFC 500‐1E after Cr(VI) and phenol treatment

et al. 2020

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Bacillus sp. SFC 500‐1E, a bacterial strain isolated from tannery sediments, is able to remove Cr(VI) and simultaneously tolerate high concentrations of phenol. In this study, we used high‐resolution microscopies, fluorescence polarization techniques, and several biochemical approaches to improve our understanding about the adaptive mechanisms of this strain to survive in the presence of Cr(VI) and phenol, both individually and simultaneously. Among adaptive strategies developed by Bacillus sp. SFC 500‐1E, an increase in bacterial size, such as length, width, and height, and ultrastructural alterations, such as electron‐dense precipitates, the presence of exopolymers, and cell lysis, are noteworthy. The exopolymers observed were consistent with the extensive biofilm formation and exopolysaccharides and extracellular protein quantification. At the cell membrane level, a rapid rigidity was induced in Cr(VI) + phenol treatment. This effect was counteracted after 16 h by changes at the level of phospholipids, mainly in the composition of fatty acids (FAs); in particular, an increase in the unsaturated fatty acid/saturated fatty acid ratio was detected. This study shows evidence of some adaptive responses displayed by Bacillus sp. SFC 500‐1E, which allows it to survive in stressful conditions.

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Identification of the main mechanisms involved in the tolerance and bioremediation of Cr(VI) by Bacillus sp. SFC 500-1E

Cited by 50 publications

References 54 publications

Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium—a proteomic approach

Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium—a proteomic approach

Microbial Chromate Reductases: Novel and Potent Mediators in Chromium Bioremediation-A Review

Morphological and structural response of Bacillus sp. SFC 500‐1E after Cr(VI) and phenol treatment

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