Cellular response of Brevibacterium casei #NIOSBA88 to arsenic and chromium—a proteomic approach

Abstract

Cellular response against different heavy metal stress differs with the metal. Arsenic and chromium are heavy metals and toxic to living systems. The concentration of these metals in seawater is very low. However, due to their solubility in nature, they actively enter cells via various transport mechanisms and cause damage to the cells. Brevibacterium casei #NIOSBA88, a marine-derived, gram-positive isolate was multi-metal tolerant. Proteomic analysis of this isolate in response to arsenic and chromium resulted in the identification of total 2549 proteins, out of which 880 proteins were found to be commonly expressed at 750 mgL −1 arsenic and 100 mgL −1 chromium and in absence of both the metals. In contrast, 533, 212, and 270 proteins were found to be unique in the absence of any metal, 750 mgL −1 of arsenic and 100 mgL −1 of chromium respectively. Proteins such as antibiotic biosynthesis monooxygenase, ArsR family transcriptional regulator, cytochrome C oxidase subunit II, and thioredoxin reductase were exclusively expressed only in response to arsenic and chromium. Other proteins like superoxide dismutase, lipid hydroperoxide reductase, and thioredoxin-disulfide reductase were found to be upregulated in response to both the metals. Most of the proteins involved in the normal cell functioning were found to be downregulated. Major metabolic functions affected include amino acid metabolism, carbohydrate metabolism, translation, and energy metabolism. Peptide mass fingerprinting of Brevibacterium casei #NIOSBA88 exposed to arsenic and chromium respectively revealed the deleterious effect of these metals on the bacterium and its strategy to overcome the stress.

Discerning the role of a functional arsenic-resistance cassette in the evolution and adaptation of a rice pathogen

“…The majority of the genes encoding protein translational machinery (ribosomal proteins) and proteins involved in amino acid metabolism were downregulated in response to arsenite. This finding is consistent with previous studies showing a decline in the rate of protein synthesis in response to arsenite As(III) exposure [ 52–54 ]. In addition, genes involved in oxidative phosphorylation pathways such as ATP synthase subunits A, C, δ and β, cytochrome ubiquinol oxidase subunits, and genes involved in intracellular protein transport, including secD , secF , secG and secE , were also downregulated in response to arsenite treatment.…”

“…The transcriptome data also showed upregulation of four DEGs, CheW, CheA, MotD and methyl-accepting chemotaxis protein, suggesting a role in the chemotaxis response towards arsenite. Upregulation of genes involved in carbohydrate metabolism pathways was also in agreement with previous studies [51, 52].…”

Cyanide Biodegradation by a Native Bacterial Consortium and Its Potential for Goldmine Tailing Biotreatment

“…These compounds are usually found in mine tailings [5]. All five genera have previously been reported to be resistant to several heavy metals, such as chromium, nickel, zinc, copper, lead, cadmium, cobalt and mercury [36,37,[46][47][48][49][50][51][52]. This characteristic renders them potentially suitable for the biotreatment of gold mine tailings.…”

Molecular Insight Into Key Eco-Physiological Process in Bioremediating and Plant-Growth-Promoting Bacteria

“…and Exiguobacterium spp. shows that under metal stress [in the presence of 750 mg L −1 arsenic; As(III) and As(V)] conditions, various housekeeping proteins (ribosomal proteins or lipid metabolism-related proteins) are downregulated while proteins like superoxide dismutase, thioredoxin disulfide reductase, lipid hydroperoxide reductase, ArsR, ArsA, Cdr (encoding Co-enzyme A disulfide reductase), and ABC transporter are be upregulated (Castro-Severyn et al, 2019;Shah and Damare, 2020). Additionally, transcriptome analysis by Liu et al (2020) also revealed the upregulation of areB gene in plant growth-promoting Paenibacillus polymyxa YCO136 in the presence of root exudates of tobacco.…”