Identification of the Genomic Constitution ofMusaL. Lines (Bananas, Plantains and Hybrids) Using Molecular Cytogenetics

Osuji, Julian O.; Harrison, G. E.; Crouch, Jonathan H.; Heslop-Harrison, J. S.

doi:10.1006/anbo.1997.0516

Cited by 67 publications

(47 citation statements)

References 22 publications

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“…The genomes A from M. acuminata and B from M. balbisiana have been distinguished by the molecular genetic method of in situ hybridization (Osuji et al 1997). Fluorescence in situ hybridization has also been used to localize ribosomal genes as well as telomere-like DNA sequences on the mitotic chromosomes of Musa species, cultivars and hybrids (Osuji 1998).…”

Section: Introductionmentioning

confidence: 99%

Comparative kayomorphology of edibleMusacultivars of West Bengal

Ghosh¹,

Das²,

Ghorai

et al. 2013

Caryologia

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Seedless cultivars of banana are generally triploids, and diploids or tetraploids are rarer. In the present study, the karyomorphology of eight edible Musa cultivars of West Bengal was investigated for the first time to contribute to a better understanding of chromosome features and chromosome changes in the way of variation. Among the eight cultivars, seven were triploid and only the eighth seeded cultivar was diploid, all with basic chromosome number of x = 11. Karyotypic differences were observed between the cultivars but not enough to serve as a marker for each cultivar. Karyotype analysis has shown predominance of chromosomes with centromeres in median position with a few submedian centromeres. This report is the first of its kind for Musa cultivars where comparative karyotyping indicate that minor structural alterations of the chromosomes may have resulted in the genetic variability within the cultivated bananas, giving rise to numerous varieties or cultivars.

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Section: Introductionmentioning

confidence: 99%

Comparative kayomorphology of edibleMusacultivars of West Bengal

Ghosh¹,

Das²,

Ghorai

et al. 2013

Caryologia

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“…Pratia chromosomes and C-values are small (Murray et al 1992), and we have also found that there is some cross-hybridization when the two parental species are used as probes in our putative hybrids. Variation in the extent of hybridization with whole genomic probes, usually with greatest hybridization in sites associated with centromeres, which are usually flanked by highly repeated sequences, has also been observed in other plants with small chromosomes (Fahleson et al 1997;Osuji et al 1997).…”

Section: Discussionmentioning

confidence: 91%

Polyploidy, hybridization and evolution inPratia(Campanulaceae)

Murray¹,

Datson²,

Lai³

et al. 2004

New Zealand Journal of Botany

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Evolution in the genus Pratia (Campanulaceae) has been studied using a variety of techniques. The distribution of chromosome races in P. angulata has been plotted and results suggest that although they generally occupy different geographic areas there are large areas of overlap. No consistent morphological differences were found between these chromosome races. The existence of hybrids between P. angulata and P. perpusilla with 1 1x and 13x chromosome numbers has been established using genomic in situ hybridization (GISH), and random amplified polymorphic DNA (RAPD) analysis suggests several independent origins for these hybrids. Artificial hybridization studies showed a high degree of compatibility in almost all the combinations that were attempted. DNA sequences derived from both the nuclear genome (internal transcribed spacer of the rDNA locus, ITS) and chloroplast genomes (trnL intron and trnL-trnF spacer) were used to construct phylogenies. Colensoaphysaloides has been shown to be distinct from the New Zealand species of Pratia and was used as an outgroup to root the phylogenetic trees. A significant finding is that P. macrodon is sister to the other New Zealand species. DNA B04008; Online publication date 9 December 2004 Received 18 March 2004; accepted 14 June 2004sequence data also confirm the hybrid origin of the 1 1x and 13x plants and show that P. perpusilla is the maternal parent of the 1 1x plants and P. angulata is the maternal parent of the 13x ones. The origin of the putative hybrid plants with the12x chromosome number remains to be elucidated.

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“…FISH (fluorescent in situ hybridization) studies (Ortiz & Vuylsteke, 1994) showed that there was a high degree of cross-hybridization between the A and B genomes suggesting that the two genomes are incompletely differentiated and share common DNA sequences. Osuji, Harrison, Crouch, and Heslop-Harrison (1997) also showed greater levels of cross hybridization between the T and the A or B genomes. The intensity of the cross hybridization may be a reflection of the sequence homologies and affinities between the genomes .…”

Section: Discussmentioning

confidence: 95%

“…While the implication in previous studies (Heslop-Harrison & Schwarzacher, 2007;Howell, Newbury, Swennen, Whithers, & Ford-Lloyd, 1994;Nwakanma, Pillay, Okoli, & Tenkouano, 2003;Nair, Teo, & Schwarzacher, Heslop-Harisson, 2005) alluded marked differentiation of the A and B genomes, the SC3 primers developed in this study from the A genome do not suggest such a differentiation. Evidence for the lack of complete differentiation of the A and B genomes of banana does exist in the literature (Ortiz & Vuylsteke, 1994;Osuji, Harrison, Crouch, & Heslop-Harrison, 1997;D'Hont, Paget-Goy, Escoute, & Carreel, 2000;Khayat, 2004;Boonruangrod, Desai, Fluch, Berenyi, & Burg, 2008;Jeridi et al, 2011Jeridi et al, , 2012Cizkova et al, 2013;De Jesus et al, 2013). The first study that alluded to the lack of differentiation between the A and B genomes was that of (Nair, Teo, Schwarzacher, & Heslop-Harisson, 2005).…”

Section: Discussmentioning

confidence: 99%

SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

Mabonga¹,

Pillay²

2017

JAS

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Bananas (Musa spp. L.) are grouped on the basis of their genomic origins in relation to Musa acuminata (A genome) and M. balbisiana (B genome). The two ancestral wild seeded diploid species evolved in vastly different geographical areas and contributed several agronomic traits towards the present genetic composition of cultivated bananas. Most cultivated bananas are triploid (AAA, AAB and ABB), some are diploid (AA, BB and AB) and a few are tetraploids (AAAA, AAAB, AABB and ABBB). Limitations on the correct identification of the A and B genomes in Musa have generated need for the development of new and more reliable techniques. Distinguishing the A and the B genome remains practically and theoretically important for banana breeders. The aim of the research was to develop a DNA based A genome specific marker for the identification of the A genome in bananas. A putative marker (600 bp) specific to the A genome was identified by Random Amplified Polymorphic DNA (RAPD) technique. A sequence characterised amplified region (SCAR) marker was developed from the RAPD amplicon. The SCAR primers annealed a 500 bp fragment specific to the A genome in a sample of 22 randomly selected homo-and heterogenomic A genome containing accessions representing different genome combinations. The 500 bp SCAR marker is useful for the identification of the A genome. However an additional 700 bp fragment annealed in all M. balbisiana genotypes and in five of the eight heterogenomic accessions, suggesting lack of differentiation between the A and B genome. This study has provided a 500 bp A genome SCAR marker and recent evidence that the A and B genomes of banana may not be as differentiated as previously considered.

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Identification of the Genomic Constitution ofMusaL. Lines (Bananas, Plantains and Hybrids) Using Molecular Cytogenetics

Cited by 67 publications

References 22 publications

Comparative kayomorphology of edibleMusacultivars of West Bengal

Comparative kayomorphology of edibleMusacultivars of West Bengal

Polyploidy, hybridization and evolution inPratia(Campanulaceae)

SCAR Marker for the A Genome of Bananas (Musa spp. L.) Supports Lack of Differentiation between the A and B Genomes

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