Identification of the Extracellular Nuclease Influencing Soaking RNA Interference Efficiency in Bursaphelenchus xylophilus

Wang, Ruijiong; Li, Yongxia; Li, Dongzhen; Zhang, Wei; Wang, Xuan; Wen, Xiaojian; Liu, Zhenkai; Feng, Yuqian; Zhang, Xingyao

doi:10.3390/ijms232012278

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…However, the critical pathogenic factor of PWD is still unknown. In recent years, research on the functional genes of PWNs has developed rapidly, and some essential genes have been identified using RNAi and other new technologies [57,58].…”

Section: Unclear Pathogenesismentioning

confidence: 99%

Pine Wilt Disease in Northeast and Northwest China: A Comprehensive Risk Review

Zhang

et al. 2023

Forests

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Pine wilt disease (PWD) caused by the pine wood nematode (PWN) is China’s most serious forest disease in recent decades. Its complex disease system with unclear pathogenesis and differentiated pathogenicity makes it challenging to manage and control. PWD has spread rapidly in Northeast and Northwest China in recent years, exceeding previous predictions and the traditional understanding of its potential distribution. This paper reviewed the comprehensive risk of PWD in Northeast and Northwest China through the occurrence of influencing factors. Regarding PWN’s temperature resistance, all regions of China are suitable for PWD. In terms of altitude, the PWD system is mainly distributed below 1000 m. In China, Pinus and Larix species are confirmed host trees of PWN, while Monochamus alternatus and M. saltuarius are confirmed vector insects of PWN. In this case, PWD may occur in all low-altitude pine forests in China. Northeast China has a higher risk than Northwest China due to its distribution of suitable host trees and vector beetles at low altitudes. To confront the threat, two scientific bottlenecks are required to be demonstrated. The first is the pathogenicity of Chinese PWN strains to Chinese Picea and Abies species. The other is the geographical distribution and highest altitude distribution of other Monochamus species in the Palaearctic region of China and their PWN transmission ability. We also suggest strengthening plant quarantining in Northeast China. In conclusion, this review comprehensively analyzes the risk of PWD in Northeast and Northwest China in terms of temperature, altitude, host trees, and vector insects. It provides a new perspective and reference for the management and research of PWD in China.

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Section: Unclear Pathogenesismentioning

confidence: 99%

Pine Wilt Disease in Northeast and Northwest China: A Comprehensive Risk Review

Zhang

et al. 2023

Forests

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“…The experiment was performed with 3 biological replicates and 4 technical replicates. The relative expression of genes was quantified using the 2 −∆∆CT method based on cycle threshold (Ct) and dissolution profiles, with efficiency correction normalized to β-actin [39]. It was determined by RT-qPCR that the relative expression levels of β-actin in the test nematodes at four stages were consistently maintained.…”

Section: Validation Of Rt-qpcrmentioning

confidence: 99%

Analysis of DNA Methylation Differences during the JIII Formation of Bursaphelenchus xylophilus

Wang,

Li,

Liu

et al. 2023

CIMB

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DNA methylation is a pivotal process that regulates gene expression and facilitates rapid adaptation to challenging environments. The pinewood nematode (PWN; Bursaphelenchus xylophilus), the causative agent of pine wilt disease, survives at low temperatures through third-stage dispersal juvenile, making it a major pathogen for pines in Asia. To comprehend the impact of DNA methylation on the formation and environmental adaptation of third-stage dispersal juvenile, we conducted whole-genome bisulfite sequencing and transcriptional sequencing on both the third-stage dispersal juvenile and three other propagative juvenile stages of PWN. Our findings revealed that the average methylation rate of cytosine in the samples ranged from 0.89% to 0.99%. Moreover, we observed significant DNA methylation changes in the third-stage dispersal juvenile and the second-stage propagative juvenile of PWN, including differentially methylated cytosine (DMCs, n = 435) and regions (DMRs, n = 72). In the joint analysis of methylation-associated transcription, we observed that 23 genes exhibited overlap between differentially methylated regions and differential gene expression during the formation of the third-stage dispersal juvenile of PWN. Further functional analysis of these genes revealed enrichment in processes related to lipid metabolism and fatty acid synthesis. These findings emphasize the significance of DNA methylation in the development of third-stage dispersal juvenile of PWN, as it regulates transcription to enhance the probability of rapid expansion in PWN.

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Pine wilt disease: what do we know from proteomics?

Cardoso,

Manadas,

Abrantes

et al. 2024

BMC Plant Biol

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Pine wilt disease (PWD) is a devastating forest disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, a migratory endoparasite that infects several coniferous species. During the last 20 years, advances have been made for understanding the molecular bases of PWN-host trees interactions. Major advances emerged from transcriptomic and genomic studies, which revealed some unique features related to PWN pathogenicity and constituted fundamental data that allowed the development of postgenomic studies. Here we review the proteomic approaches that were applied to study PWD and integrated the current knowledge on the molecular basis of the PWN pathogenicity. Proteomics has been useful for understanding cellular activities and protein functions involved in PWN-host trees interactions, shedding light into the mechanisms associated with PWN pathogenicity and being promising tools to better clarify host trees PWN resistance/susceptibility.

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Identification of the Extracellular Nuclease Influencing Soaking RNA Interference Efficiency in Bursaphelenchus xylophilus

Cited by 4 publications

References 39 publications

Pine Wilt Disease in Northeast and Northwest China: A Comprehensive Risk Review

Pine Wilt Disease in Northeast and Northwest China: A Comprehensive Risk Review

Analysis of DNA Methylation Differences during the JIII Formation of Bursaphelenchus xylophilus

Pine wilt disease: what do we know from proteomics?

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