Identification of the delayed rectifier potassium channel, Kv1.6, in cultured astrocytes

Smart, Sharon L.; Bosma, Martha M.; Tempel, Bruce L.

doi:10.1002/(sici)1098-1136(199706)20:2<127::aid-glia4>3.0.co;2-6

Cited by 43 publications

(27 citation statements)

References 18 publications

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“…This is the a-isoform, since KvC antibody did not label astrocytes very well. The finding compares to recent reports on the presence of voltage-dependent potassium currents in neuroglia (Anderova et al, 2004;Chung et al, 1998;Smart et al, 1997;Bekar and Walz, 2002), although none identified the presence of Kv3.1. The resolution of the present material leaves open the question of the submicroscopic localization of this protein in the astrocytic processes and its relation to growing axons and synapses.…”

Section: The Staining Of Astrocytescontrasting

confidence: 79%

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Feng

Morest

2006

Hearing Research

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Section: The Staining Of Astrocytescontrasting

confidence: 79%

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Feng

Morest

2006

Hearing Research

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“…DTX-I is the selective blocker of the depolarization-activated K + current produced by the activation of K + channels containing Kv1.1 [126], Kv1.2 [127] and/or Kv1.6 subunits [128]; for a review see [129]. It has been established that (similarly to other cells of the auditory pathway) the rapidly adapting somatic action potential firing of type I SGCs (i.e., the fast adaptation of the cells) could be effectively inhibited by the application of low doses of 4-aminopyridine (4-AP) or by dendrotoxin (DTX) [94,125].…”

Section: Dendrotoxin-sensitive K + Currentmentioning

confidence: 99%

Spiral ganglion neurones: an overview of morphology, firing behaviour, ionic channels and function

Rusznák

Szücs

2008

Pflugers Arch - Eur J Physiol

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The spiral ganglion cells provide the afferent innervation of the hair cells of the organ of Corti. Ninety-five percent of these cells (termed type I spiral ganglion neurones) are in synaptic contact with the inner hair cells, whereas about 5% of them are type II cells, which are responsible for the sensory innervation of the outer hair cells. To understand the function of the spiral ganglion neurones, it is important to explore their membrane properties, understand their activity patterns and describe the variety of ionic channels determining their behaviour. In this review, a brief description is given of the various experimental methods that allow the investigation of the spiral ganglion cells, followed by the discussion of their action potential firing patterns and ionic conductances. The presence, distribution and significance of the K(+) currents of the spiral ganglion cells are specifically addressed, along with the introduction of the putative subunit compositions of the relevant voltage-gated K(+) channels.

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“…Kv1.1 and Kv1. 6 have been identified by RT-PCR in mouse cortical astrocytes [11]. Expression of Kv1.5 was demonstrated by immunohistochemistry both in brain sections and in cultured spinal cord astrocytes from the rat.…”

Section: Introductionmentioning

confidence: 99%

The Kv1.3 potassium channel is localized to the cis‐Golgi and Kv1.6 is localized to the endoplasmic reticulum in rat astrocytes

2014

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The functions of voltage‐gated potassium (Kv) channels in neurons have been well defined, whereas their roles in glial cells are not fully understood. Kv1.1, Kv1.3 and Kv1.6 are endogenously expressed in C6 astrocytoma cells, but their trafficking and subcellular localization have not been well studied. In C6 cells, Kv1.1 was localized to the cell surface, Kv1.3 was predominantly localized in the cis‐Golgi, and Kv1.6 was enriched in the endoplasmic reticulum. Disruption of the Golgi stacks with brefeldin A treatment redirected Kv1.3 to the endoplasmic reticulum, further confirming that Kv1.3 was localized in the Golgi. Denaturing and reducing immunoblot analysis identified an expected Kv1.3 monomer and an unexpected Kv1.3 dimer/aggregate. These two forms had different protein half‐lives: that of the monomer form T1/2 was 5.1 h, whereas the dimer/aggregate form was stable over the 8‐h measurement period. The Kv1.3 dimer/aggregate form on immunoblots appeared to be correlated with its Golgi retention, based on examination with several cell types that expressed Kv1.3. Glycosidase treatment showed that Kv1.3 contained complex‐type N‐glycans terminated with sialic acids, suggesting that Kv1.3 had traveled to the trans‐Golgi network for sialylation before it was recycled to the cis‐Golgi for retention. Inhibition of N‐glycosylation did not affect Kv1.3 localization, indicating that N‐glycans did not play a role in its Golgi retention. Thus, Kv1.3 appears to be distributed to the cis‐Golgi membrane of rat astrocytes in a similar way as a Golgi resident protein, and this unusual distribution appears to be correlated with its SDS/2‐mercaptoethanol‐resistant dimer/aggregate forms on immunoblots.

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Identification of the delayed rectifier potassium channel, Kv1.6, in cultured astrocytes

Cited by 43 publications

References 18 publications

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Spiral ganglion neurones: an overview of morphology, firing behaviour, ionic channels and function

The Kv1.3 potassium channel is localized to the cis‐Golgi and Kv1.6 is localized to the endoplasmic reticulum in rat astrocytes

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