Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO

Jarrell, Ken F.; Kropinski, Andrew M.

doi:10.1128/jvi.23.3.461-466.1977

Cited by 43 publications

(21 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The R and H series of mutants were selected by screening for mutants resistant to the smooth-LPS-specific phages E79 (29) and 2 Lindberg (1), respectively, according to the method used by Jarrell and Kropinski (27). The Gt series of mutants were isolated by growing serotype 06 in the presence of progressively higher concentrations of gentamicin in Mueller-Hinton broth (Difco Laboratories) as described by Galbraith et al (18).…”

Section: Methodsmentioning

confidence: 99%

Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6

et al. 1994

View full text Add to dashboard Cite

Well-characterized rough mutants are important for the understanding of structures, functions, and biosynthesis of lipopolysaccharide (LPS) in gram-negative organisms. In this study, three series of Pseudomonas aeruginosa LPS-deficient mutants, namely PAC strains derived from serotype 03, AK strains derived from strain PA01 (serotype 05), and serotype 06-derived mutants were subjected to biochemical analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining as well as immunochemical characterization using LPS-specific monoclonal antibodies. The 0-side-chain deficiency among the 06-derived mutants was also examined, and three mutants, A28, R5, and H4, were subsequently chosen for the elucidation of component sugars of the core structure of serotype 06 LPS. LPS of strain A28 has L-rhamnose and proportionally higher amounts of D-glucose, a feature shared by the 05-derived mutant, strain AK1401

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6

et al. 1994

View full text Add to dashboard Cite

show abstract

“…Accordingly, phages can be highly specific for a given O antigen serotype, or can have a broader host range if they recognize more conserved constituents of LPS [137]. A number of LPS-specific phages that target P. aeruginosa have been described in the literature as well as phage-resistant mutants arising from mutations in LPS biosynthesis genes [138][139][140][141][142][143][144][145][146][147]. In other cases, phage resistance may arise when temperate bacteriophages encode proteins that modify the O antigen, conferring resistance to superinfection [148].…”

Section: Phages and Pyocinsmentioning

confidence: 99%

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

2019

View full text Add to dashboard Cite

The major constituent of the outer membrane of Gram-negative bacteria is lipopolysaccharide (LPS), which is comprised of lipid A, core oligosaccharide, and O antigen, which is a long polysaccharide chain extending into the extracellular environment. Due to the localization of LPS, it is a key molecule on the bacterial cell wall that is recognized by the host to deploy an immune defence in order to neutralize invading pathogens. However, LPS also promotes bacterial survival in a host environment by protecting the bacteria from these threats. This review explores the relationship between the different LPS glycoforms of the opportunistic pathogen Pseudomonas aeruginosa and the ability of this organism to cause persistent infections, especially in the genetic disease cystic fibrosis. We also discuss the role of LPS in facilitating biofilm formation, antibiotic resistance, and how LPS may be targeted by new antimicrobial therapies.

show abstract

“…This apparent lack of adsorption of the phages provided favorable evidence that the conversion in PAO(D3) involves LPS since this molecule is the known receptor for E79 (27) and D3 (R. E. W. Hancock, personal communication).…”

Section: Resultsmentioning

confidence: 99%

“…Phage techniques. The procedures used to titrate phage preparations, measure adsorption rates, and study the interaction of phage and LPS have been previously published (27,29,30). Isolation of the PAO(D3) lysogen.…”

Section: Methodsmentioning

confidence: 99%

O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3

Kuzio¹,

Kropinski²

1983

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The lysogenization of Pseudomonas aeruginosa PAO by phage D3 results in derivatives which are resistant to superinfection by phage D3c by virtue of the fact that homologous phage cannot adsorb to these cells. The serologically and morphologically unrelated phage E79 showed a markedly decreased adsorption rate to the lysogen PAO(D3). Since both of these phages are lipopolysaccharide specific, these results suggested lysogenic conversion of the phage receptor. The lipopolysaccharide was extracted from strain PAO by the hot phenol-water technique, but this procedure was ineffective with PAO(D3). We developed a technique involving cold trichloroacetic acid extraction, followed by ultracentrifugation, digestion of the high-speed pellet with proteinase K, and ultimate purification on CsCl step gradients. The lipopolysaccharide from the wild type had inactivating activity against D3 and E79, whereas that from PAO(D3) inactivated neither. Chromatographic analysis indicated that the convertant lipopolysaccharide was smooth, and quantitative chemical analyses of the two preparations showed no differences in the level of the major fatty acids, amino compounds, or neutral sugars. On the other hand, sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the side chains had a decreased migration rate through the gel matrix. The application of 1H and 13C nuclear magnetic resonance spectroscopic analysis revealed that the PAO side chain is chemically identical to that of serotype O:2a,d, containing 2,3-(1-acetyl-2-methyl-2-imidazolino-5,4)-2,3-dideoxy-D-mannuronic acid, 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, and 2-acetamido-2,6-dideoxy-D-galactose (D-fucosamine). The molecular basis of the conversion event was (i) the introduction of an acetyl group into position 4 of the fucosamine residue and a change in the bonding between trisaccharide repeating units from alpha 1 leads to 4 to beta 1 leads to 4.

show abstract

Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO

Cited by 43 publications

References 33 publications

Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6

Characterization of lipopolysaccharide-deficient mutants of Pseudomonas aeruginosa derived from serotypes O3, O5, and O6

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3

Contact Info

Product

Resources

About