Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

Marek, Agnieszka; Pyzik, Ewelina; Stępień–Pyśniak, Dagmara; Hauschild, Tomasz; Banach, Tomasz

doi:10.1515/bvip-2015-0035

Cited by 3 publications

(4 citation statements)

References 12 publications

(13 reference statements)

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“…A relatively new identification method using a MALDI-TOF MS system with prior formic acid extraction has provided excellent diagnostic results and reduced identification time (Seng et al, 2009;Wieser et al, 2012). MALDI-TOF MS is a rapid and accurate technique for the identification of various types of Grampositive or Gram-negative bacteria (Kosikowska et al, 2014;Marek et al, 2015;Nowakiewicz et al, 2015).…”

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confidence: 99%

Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry

Stępień–Pyśniak

Marek

Banach

et al. 2016

Acta Veterinaria Hungarica

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The aim of this study was to evaluate the frequency of occurrence of bacteria of the genus Enterococcus in poultry, to identify them by means of matrixassisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), and to analyse the antimicrobial susceptibility of the isolated strains to the drugs most frequently used in poultry. The material for the bacteriological tests was obtained mainly from the heart (97%) of the birds investigated. Of a total of 2,970 samples tested, 911 (30.7%) tested positive for Enterococcus spp. Enterococci were detected in broilers (88.1%), laying hens (5.3%), turkeys (3.9%), breeding hens (2.2%), and geese (0.4%). The most commonly identified species were Enterococcus (E.) faecalis (74.7%), E. faecium (10.1%), E. gallinarum (5.5%), E. hirae (4.6%), and E. cecorum (4.1%). The most frequent resistance properties were resistance to sulphamethoxazole/trimethoprim (88%), tylosin (71.4%), enrofloxacin (69.4%), doxycycline (67.3%), and lincomycin/spectinomycin (56.1%). Only one vancomycin-resistant Enterococcus, E. cecorum from a broiler, was found.

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confidence: 99%

Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry

Stępień–Pyśniak

Marek

Banach

et al. 2016

Acta Veterinaria Hungarica

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“…The following discs were used: enrofloxacin (ENR 30 μg), ampicillin (AMP 10 μg), (Capita, 2007, Marek et al, 2015.…”

Section: Evaluation Of the Antibiotic Susceptibility Of Bacteria By Tmentioning

confidence: 99%

“…Zones of growth inhibition were evaluated as susceptible or resistant according to the CLSI Guidelines for Susceptibility Testing. The procedures ofMarek et al (2015Marek et al ( , 2016;Dudzic et al (2016);Nowaczek et al (2019) andStępień-Pyśniak et al (2016) were followed.…”

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confidence: 99%

The Influence of a Diet Supplemented with 20% Rye and Xylanase in Different Housing Systems on the Occurrence of Pathogenic Bacteria in Broiler Chickens

Pyzik

Urban‐Chmiel

Chałabis-Mazurek

et al. 2021

Annals of Animal Science

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Sanitary conditions and diet are important elements determining the occurrence of pathogens in animals. The aim of the research was to assess the effect of an experimental diet with rye and xylanase for broiler chickens in cages and in a free-range system on the intestinal microbiome. The study was carried out in two experimental stages, the first on 224 1-d-old male Ross 308 chickens with an initial weight of 41 g, and the second on 2000 1-d-old male chickens with an initial weight of 42 g. All birds were reared to 42 d of age and fed crumbled starter (1 to 21 d) and pelleted grower–finisher (22 to 42 d) isonitrogenous and isoenergetic diets, supplemented with 20% rye and/or 200 mg/kg xylanase. Directly after slaughter, bacteria were isolated from the cloaca of birds and identified using classical microbiological methods and MALDI-TOF mass spectrometry. The antibiotic susceptibility of the bacteria was assessed by the disc diffusion method. The study showed the presence of abundant bacteria in the gut microbiome of chickens kept in both housing systems. The most frequently isolated bacteria were Escherichia coli, Enterococcus spp., Proteus spp., Campylobacter spp., and Staphylococcus spp. Antibiotic resistance was significantly higher in E. coli, Proteus spp., and Campylobacter spp. obtained from chickens from the free-range farm, but in the case of Enterococcus and Staphylococcus, resistance was higher in bacteria from caged birds. The high antibiotic resistance among pathogens of the gastrointestinal tract necessitates the search for means to control the microbiome in favour of beneficial bacteria. The significant influence of rye and xylanase on the bacterial content may be the basis for the introduction of this method to support the control of pathogens.

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“…Briefly, sequencing was performed with a total volume of 10 µl containing 0.5 µl of premix from the ABI Prism BigDye Terminator (version 3.0) ready reaction cycle sequencing kit (Applied Biosystems), 1.8 µl of buffer (400 mM Tris-HCl, 10 mM MgCl2), 10 pmol of sequencing primer, and 3 µl of the cleaned-up PCR product (Marek et al, 2015). The sequencing products were purified using ExTerminator kits (A&A Biotechnology, Poland) and analysed in an ABI PRISM 3130 genetic analyser, as specified by the manufacturer (Applied Biosystems).…”

Section: Identification Of Isolated Strains By Rpob and Dnaj Genes Sementioning

confidence: 99%

Staphylococcus simulans associated with endocarditis in broiler chickens

Stępień–Pyśniak

Wilczyński²,

Marek

et al. 2016

Avian Pathology

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This report suggests a strong association between coagulase-negative Staphylococcus simulans and endocarditis in broiler chickens of a single flock. Clinical signs included increased mortality and lameness, and some dead chickens were found on their backs. Lesions included cauliflower-like, fibrinous vegetative lesions on the left atrioventricular valve; cream-coloured, necrotic foci of varying size in the liver; and necrosis of the femoral head. Histopathological examination of the heart revealed multifocal conglomerates of bacterial colonies attached to the valvular endocardium, threads of fibrin, and inflammatory cells with the presence of heterophils. S. simulans strains were first identified by API ID32, and then confirmed with Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry and by partial sequencing of the rpoB and dnaJ genes. These bacteria were resistant to methicillin but sensitive to vancomycin and characterized by slime production and protease activity.

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Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

Cited by 3 publications

References 12 publications

Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry

Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry

The Influence of a Diet Supplemented with 20% Rye and Xylanase in Different Housing Systems on the Occurrence of Pathogenic Bacteria in Broiler Chickens

Staphylococcus simulans associated with endocarditis in broiler chickens

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